Use of glucomannan for the separation of DNA fragments by capillary electrophoresis

Izumi, Takeki; Yamaguchi, Masato; Yoneda, Kenichi; Isobe, Toshiaki; Okuyama, Tsuneo; Shinoda, Tomotaka

doi:10.1016/0021-9673(93)80643-m

Cited by 52 publications

(24 citation statements)

References 14 publications

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“…Most of them are modified polysaccharides, such as hydroxyethylcellulose (HEC) [83,84], hydroxypropylcellulose (HPC) [85,86], methycellulose, dextran [87], glucomannan [88], etc. or synthetic polymers, like polyethers or linear polyacrylamide and its derivatives.…”

Section: Polymer Solutions Used In Electrophoresis and Major Experimementioning

confidence: 99%

Sieving mechanisms in polymeric matrices

2003

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A critical review of the existing theoretical models and experimental evidences for sieving mechanisms during separation of macromolecules, paying particular attention to capillary electrophoresis applications is presented. Gel models (Ogston and reptation) have been successfully applied to highly entangled polymer solutions, where fast and efficient separations can occur. In order to account for the DNA/polymers collision-interaction mechanisms during separation in dilute solutions - characterized by a poorer resolution -, approximated analytical models have been developed. An insight in the mechanism regulating the intermediate case of moderately entangled polymer solutions, for low fields and concentrations of small multiples of the overlap concentration c*, is given by the constraint release approach. This model proposes an upper limit of size separation, increasing with matrix concentration and molecular mass. Finally, the coupling between the reptative motion of the analytes and the effect of matrix constraint release very likely plays a fundamental role in the separation mechanism and requires therefore further and deeper investigation, both theoretically and experimentally.

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Section: Polymer Solutions Used In Electrophoresis and Major Experimementioning

confidence: 99%

Sieving mechanisms in polymeric matrices

2003

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“…Konjac glucomannan has wide applications in the food industry, 3,4 in the chemical industry, 5,6 and in biochemistry. 7,8 Konjac glucomannan can be extruded into films for coating and packing applications. In recent years, exploitation of natural fruit and food preservatives, such as chitin 9 and chitosan, 10 has drawn increasing attention.…”

Section: Introductionmentioning

confidence: 99%

Characterization of konjac glucomannan–quaternized poly(4‐vinyl‐N‐butyl) pyridine blend films and their preservation effect

Liu

Xiao

2004

J of Applied Polymer Sci

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Novel polymer blends were prepared from a mixture of 2 wt % konjac glucomannan and 4 wt % quaternized poly(4-vinyl-N-butyl) pyridine (QPVP) in aqueous solution and dried at room temperature for 72 h. Their structure and properties were studied by infrared, wide-angle X-ray diffraction, scanning electron microscopy, thermogravimetric analysis, and differential scanning calorimetry. Thermal stability in the dry state was reduced with increasing content of QPVP. Compared with QPVP film, the tensile strength of the films was improved in the dry state. The maximum value of 12.74% tensile break elongation was reached when the content of QPVP was 30%. Structural analysis indicated that clear phase separation was observed when the content of QPVP was only 50%. Results from the filmcoating preservation experiments with lychee showed that this blend film had water-holding ability. The fruit weight loss rate and rot rate both decreased in various degrees. The potential uses of these novel polymer films could be as preservative films.

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“…A wide variety of polymers have been investigated, and more than ten noncross-linked polymers have been adopted as DNA separation media. They are synthetic polymers [69,70], such as LPA, and modified polysaccharides, such as celluloses [71], dextran [72], glucomannan [73], etc. The main polymer solutions are shown in Table 1 and discussed below.…”

Section: Noncross-linked Polymer Solutionsmentioning

confidence: 99%

Polymer solutions and entropic‐based systems for double‐stranded DNA capillary electrophoresis and microchip electrophoresis

Baba

2004

Electrophoresis

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We give an overview of recent development of low-viscosity polymer solutions and entropic trapping networks for double-stranded DNA (dsDNA) separations by conventional capillary electrophoresis and microchip electrophoresis. Theoretical models for describing separation mechanisms, commonly used noncross-linked polymer solutions, thermoresponsive (viscosity-adjustable) polymer solutions, and novel entropic trapping networks are included. The thermoresponsive polymer solutions can be loaded at one temperature into microchannels at lower viscosities, and used in separation at another temperature at entanglement threshold concentrations and higher viscosities. The entropic-based separations use only arrays of regular obstacles acting as size-separations and do not need viscous polymer solutions. These progresses have potential in integration to automated capillary and microfluidic chip systems, enabling better reusability of separation microchannels, much shorter DNA separation times, and higher reproducibility due to less matrix degradation.

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Use of glucomannan for the separation of DNA fragments by capillary electrophoresis

Cited by 52 publications

References 14 publications

Sieving mechanisms in polymeric matrices

Sieving mechanisms in polymeric matrices

Characterization of konjac glucomannan–quaternized poly(4‐vinyl‐N‐butyl) pyridine blend films and their preservation effect

Polymer solutions and entropic‐based systems for double‐stranded DNA capillary electrophoresis and microchip electrophoresis

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