Use of Gold Nanoparticles in a Simple Colorimetric and Ultrasensitive Dynamic Light Scattering Assay: Selective Detection of Arsenic in Groundwater

Kalluri, Jhansi Rani; Arbneshi, Tahir; Khan, Sadia Afrin; Neely, Adria; Candice, Perry; Varisli, Birsen; Washington, Marla; McAfee, Shardae; Robinson, Britinia; Banerjee, Santanu; Singh, Anant Kumar; Senapati, Dulal; Ray, Paresh Chandra

doi:10.1002/ange.200903958

Cited by 126 publications

(79 citation statements)

References 46 publications

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“…That is, we can detect 0.1 femtomole of target DNA with the help of UV-visible spectroscopy and 10 femtomoles of target DNA by the naked eye. Considering the Au NP size effect, the sensitivity of our system could be Figure 3 The correlation of UV-visible absorbance peak shift with initial target DNA concentration further improved by employing larger Au NPs because the plasmonic interaction between larger Au NPs has been shown to be significantly stronger than for 13 nm Au NPs [24,25].…”

Section: Resultsmentioning

confidence: 99%

One-pot polymerase chain reaction with gold nanoparticles for rapid and ultrasensitive DNA detection

Cai

Zhang

et al. 2010

Nano Res.

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We report a novel method for rapid, colorimetric detection of a specific deoxyribonucleic acid (DNA) sequence by carrying out a polymerase chain reaction in the presence of gold nanoparticles functionalized with two primers. Extension of the primers when the target DNA is present as a template during the polymerase chain reaction process affords the complementary sequences on the gold nanoparticle surfaces and results in the formation of gold nanoparticle aggregates with a concomitant color change from red to pinkish/purple. This method provides a convenient and straightforward solution for ultrasensitive DNA detection without any further post-treatment of the polymerase chain reaction products being necessary, and is a promising tool for rapid disease diagnostics and gene sequencing.

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Section: Resultsmentioning

confidence: 99%

One-pot polymerase chain reaction with gold nanoparticles for rapid and ultrasensitive DNA detection

Cai

Zhang

et al. 2010

Nano Res.

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“…DLS and ELS were carried out as described in refs. [82][83][84][85][86]. SDS/PAGE and Western blotting analyses were performed according to the procedures outlined in refs.…”

Section: Methodsmentioning

confidence: 99%

Nanospherical arabinogalactan proteins are a key component of the high-strength adhesive secreted by English ivy

Huang

Wang

et al. 2016

Proc. Natl. Acad. Sci. U.S.A.

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Over 130 y have passed since Charles Darwin first discovered that the adventitious roots of English ivy (Hedera helix) exude a yellowish mucilage that promotes the capacity of this plant to climb vertical surfaces. Unfortunately, little progress has been made in elucidating the adhesion mechanisms underlying this high-strength adhesive. In the previous studies, spherical nanoparticles were observed in the viscous exudate. Here we show that these nanoparticles are predominantly composed of arabinogalactan proteins (AGPs), a superfamily of hydroxyproline-rich glycoproteins present in the extracellular spaces of plant cells. The spheroidal shape of the AGP-rich ivy nanoparticles results in a low viscosity of the ivy adhesive, and thus a favorable wetting behavior on the surface of substrates. Meanwhile, calciumdriven electrostatic interactions among carboxyl groups of the AGPs and the pectic acids give rise to the cross-linking of the exuded adhesive substances, favor subsequent curing (hardening) via formation of an adhesive film, and eventually promote the generation of mechanical interlocking between the adventitious roots of English ivy and the surface of substrates. Inspired by these molecular events, a reconstructed ivy-mimetic adhesive composite was developed by integrating purified AGP-rich ivy nanoparticles with pectic polysaccharides and calcium ions. Information gained from the subsequent tensile tests, in turn, substantiated the proposed adhesion mechanisms underlying the ivy-derived adhesive. Given that AGPs and pectic polysaccharides are also observed in bioadhesives exuded by other climbing plants, the adhesion mechanisms revealed by English ivy may forward the progress toward understanding the general principles underlying diverse botanic adhesives.ivy nanoparticle | ivy adhesive | arabinogalactan protein | adhesion mechanism | reconstructed adhesive A lthough there is a growing interest in exploring mechanisms regulating a variety of adhesive behaviors in the animal kingdom (1-6), the molecular basis allowing creeping plants, such as English ivy (Hedera helix), to generate sufficient adhesive force, aiding in clinging to vertical surfaces, is rarely discussed (Fig. 1A). Previous studies have emphasized mechanical strategies exploited by multiple climbing organs that evolve in plants (7-11). Nevertheless, the role of the glue-like viscous exudates that are observed on the majority of these organs and that cement the plants to the substrates has been less explored (10,12,13). Diverse polysaccharides and glycoproteins, comprising mucilaginous pectins, arabinogalactans, arabinogalactan proteins (AGPs), and many others, have been identified to be the predominant components in these adhesive substances (14-17); however, the molecular mechanisms underlying the high-strength adhesion remain elusive.By means of atomic force microscopy (AFM), bulk spherical organic nanoparticles have been observed in the exudates derived from the root hairs of English ivy (18-20). These proteinaceous nanoparticles are presum...

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“…24−31 Surprisingly, studies on the use of AuNPs as optical probes to detect arsenic are sparse. 32,33 By successively modifying the surface of AuNPs with glutathione, dithiothreitol and cysteine, Ray's group developed a label-free optical probe for colorimetric detection of arsenic at μg L −1 level by naked eyes. 32 In that procedure, the total amount of As III and As V was quantified because As V was inevitably reduced to As III by dithiothreitol, which was used as an essential modifier.…”

Section: ■ Introductionmentioning

confidence: 99%

“…32,33 By successively modifying the surface of AuNPs with glutathione, dithiothreitol and cysteine, Ray's group developed a label-free optical probe for colorimetric detection of arsenic at μg L −1 level by naked eyes. 32 In that procedure, the total amount of As III and As V was quantified because As V was inevitably reduced to As III by dithiothreitol, which was used as an essential modifier. To the best of our knowledge, speciation test of inorganic As III and As V with a single AuNP probe has not been reported yet, and it remains a great challenge to test the two inorganic arsenic species at low μg L −1 levels with naked eyes.…”

Section: ■ Introductionmentioning

confidence: 99%

Colorimetric Au Nanoparticle Probe for Speciation Test of Arsenite and Arsenate Inspired by Selective Interaction between Phosphonium Ionic Liquid and Arsenite

Tan

Liu

Yin

et al. 2014

ACS Appl. Mater. Interfaces

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The exposure of millions of people to unsafe levels of arsenite (As III ) and arsenate (As V ) in drinking waters calls for the development of low-cost methods for on-site monitoring these two arsenic species in waters. Herein, for the first time, tetradecyl (trihexyl) phosphonium chloride ionic liquid was found to selectively bind with As III via extended Xray absorption fine structure (EXAFS) analysis. Based on the finding, an As III -specific probe was developed by modifying gold nanoparticles with the ionic liquid. Futhermore, Hofmeister effect was primarily observed to significantly affect the sensitivity of gold nanoparticle probe. With the colorimetric probe, we developed a protocol for naked eye speciation test of As III and As V at levels below the World Health Organization (WHO) guideline of 10 μg L −1 . This method featured with high tolerance to common coexisting ions such as 10 mM PO 4 3− , and was validated by assaying certified reference and environmental water samples. KEYWORDS: arsenic, ionic liquid, interaction, gold nanoparticle, Hofmeister effect, visual test ■ INTRODUCTIONArsenic shows various adverse health effects including carcinogenic disease relative to skin, lung, and kidney. 1−3 More than 140 million people worldwide are exposed to superfluous arsenic, mainly by drinking ground waters polluted by inorganic arsenic that are more toxic than the organic ones. 4,5 Although As III and As V are coexisting and undergo mutual transformation in groundwater, As III is usually the main form and more toxic than As V . 6 Consequently, monitoring of inorganic arsenic species in waters is of prior interest in arsenic detection. Numerous instrumental methods have been developed to quantify trace arsenic, 7−10 but the requirement of bulk equipment and strict laboratory conditions restricts their application in field analysis. On the contrary, the colorimetric or visual methods are quite suitable for field determination. Because of their poor sensitivity, however, traditional visual probes are incapable of detecting As III and As V at 10 μg L −1 level, which is the arsenic guideline value in drinking water required by the World Health Organization (WHO) and U.S. Environmental Protection Agency (EPA). Additionally, the fairly poor tolerance to interferences of these probes restricts their application in real sample analysis. 11−13 Several arsenic testing field kits are able to semiquantify 10 or 50 μg L −1 As, but most of them fail to meet the requirements of an ideal test kit for As. 14−16 Gold nanoparticles (AuNPs) are widely used as optical probes in colorimetric detection in recent years, 17−19 because of their higher extinction coefficient in the visible region and thus higher sensitivity in comparison to the organic probes. Since the pioneering work of the Mirkin group, 20 AuNPs have served as optical probes for sensing cations, anions, small organic compounds, and biological molecules, 21−23 with Hg 2+ as one of the most attractive target analytes. 24−31 Surprisingly, studies on the u...

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Use of Gold Nanoparticles in a Simple Colorimetric and Ultrasensitive Dynamic Light Scattering Assay: Selective Detection of Arsenic in Groundwater

Cited by 126 publications

References 46 publications

One-pot polymerase chain reaction with gold nanoparticles for rapid and ultrasensitive DNA detection

One-pot polymerase chain reaction with gold nanoparticles for rapid and ultrasensitive DNA detection

Nanospherical arabinogalactan proteins are a key component of the high-strength adhesive secreted by English ivy

Colorimetric Au Nanoparticle Probe for Speciation Test of Arsenite and Arsenate Inspired by Selective Interaction between Phosphonium Ionic Liquid and Arsenite

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