Use of hi ii-elite inbred hybrids in Agrobacterium-based transformation of maize

Horn, Michael E.; Harkey, Robin; Vinas, Amanda; Drees, Carol; Barker, Donna K.; Lane, Jeffrey R.

doi:10.1079/ivp2006776

Cited by 19 publications

(16 citation statements)

References 20 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…The model species Arabidopsis thaliana can be easily transformed via the oral dip method with Agrobacteria delivery of genetic material, a process which can generate stable transgenic seed within 3-5 weeks [12]. In contrast, maize transformation methods are more laborious and time consuming, taking up to 6 months after the transformation event to generate transgenic seed [13][14][15][16][17] . Typically, immature embryo-derived callus tissue is transformed either by particle bombardment or Agrobacterium-mediated delivery of transgenes and both processes have a transformation rate e ciency of about 12-30% [13].…”

Section: Introductionmentioning

confidence: 99%

A CRISPR/dCas9 toolkit for functional analysis of maize genes

Gentzel

Park

Bellizzi

et al. 2020

Preprint

View full text Add to dashboard Cite

Background: The Clustered Regularly Interspaced Short Palindromic Repeats (CRISPR)/Cas9 system has become a powerful tool for functional genomics in plants. The RNA-guided nuclease can be used to not only generate precise genomic mutations, but also to manipulate gene expression when present as a deactivated protein (dCas9). Results: In this study, we describe a vector toolkit for analyzing dCas9-mediated activation (CRISPRa) or inactivation (CRISPRi) of gene expression in maize protoplasts. An improved maize protoplast isolation and transfection method is presented, as well as a description of dCas9 vectors to enhance or repress maize gene expression. Conclusions: We anticipate that this maize protoplast toolkit will streamline the analysis of gRNA candidates and facilitate genetic studies of important trait genes in this transformation-recalcitrant plant.

show abstract

Section: Introductionmentioning

confidence: 99%

A CRISPR/dCas9 toolkit for functional analysis of maize genes

Gentzel

Park

Bellizzi

et al. 2020

Preprint

View full text Add to dashboard Cite

show abstract

“…The genomic Lr34res construct under the wheat native promoter and terminator in the binary vector p6U ( p6U:gLr34res ; Risk et al ., ) was transformed into the maize Hi‐II (A188 x B73) hybrid (Horn et al ., ) using Agrobacterium tumefaciens ‐mediated transformation according to a protocol previously established (Hensel et al ., ; Van der Linde et al ., ). Fifty‐five T0‐plants were recovered and the identification of the Lr34res transformants was done by PCR on genomic DNA using the cssfr1 marker (Lagudah et al ., ).…”

Section: Methodsmentioning

confidence: 99%

The durable wheat disease resistance gene Lr34 confers common rust and northern corn leaf blight resistance in maize

Sucher

Böni

Yang

et al. 2016

Plant Biotechnology Journal

View full text Add to dashboard Cite

SummaryMaize (corn) is one of the most widely grown cereal crops globally. Fungal diseases of maize cause significant economic damage by reducing maize yields and by increasing input costs for disease management. The most sustainable control of maize diseases is through the release and planting of maize cultivars with durable disease resistance. The wheat gene Lr34 provides durable and partial field resistance against multiple fungal diseases of wheat, including three wheat rust pathogens and wheat powdery mildew. Because of its unique qualities, Lr34 became a cornerstone in many wheat disease resistance programmes. The Lr34 resistance is encoded by a rare variant of an ATP‐binding cassette (ABC) transporter that evolved after wheat domestication. An Lr34‐like disease resistance phenotype has not been reported in other cereal species, including maize. Here, we transformed the Lr34 resistance gene into the maize hybrid Hi‐II. Lr34‐expressing maize plants showed increased resistance against the biotrophic fungal disease common rust and the hemi‐biotrophic disease northern corn leaf blight. Furthermore, the Lr34‐expressing maize plants developed a late leaf tip necrosis phenotype, without negative impact on plant growth. With this and previous reports, it could be shown that Lr34 is effective against various biotrophic and hemi‐biotrophic diseases that collectively parasitize all major cereal crop species.

show abstract

“…They were combined with log phase A. tumefaciens EHA101 containing the cellulase vectors. Samples were vortexed, sonicated for 30 s (Trick and Finer 1997) and placed on solidified co-cultivation medium plates containing MS salts and MS vitamins, as described by Horn et al (2006). Plates were incubated for 3, 4, or 5 d at 18°C in the dark.…”

Section: Methodsmentioning

confidence: 99%

A method for transient expression in maize endosperm

Requesens

Egelkrout

Devaiah

et al. 2010

In Vitro Cell.Dev.Biol.-Plant

View full text Add to dashboard Cite

The ability to screen the functionality of gene constructs in a transient system of appropriate tissues informs the researcher of the potential success of stable transformation. For this purpose, we developed a transient system to test the functionality of endosperm-preferred promoters in maize. Two endosperm-preferred promoters from rice (a globulin and a glutelin promoter) were employed. Ears of Zea mays L. were harvested at 17 d after pollination, surface sterilized and the endosperm excised. Using Agrobacterium tumefaciens co-cultivation and sonication, transient expression of the target genes was detected after 4 and 5 d. We demonstrate expression of CBH I and CHB II (both exo-cellulases) up to 1.7% TSP, under the rice globulin and glutelin promoters.

show abstract

Use of hi ii-elite inbred hybrids in Agrobacterium-based transformation of maize

Cited by 19 publications

References 20 publications

A CRISPR/dCas9 toolkit for functional analysis of maize genes

A CRISPR/dCas9 toolkit for functional analysis of maize genes

The durable wheat disease resistance gene Lr34 confers common rust and northern corn leaf blight resistance in maize

A method for transient expression in maize endosperm

Contact Info

Product

Resources

About