Use of <i>12p</i> and <i>36p</i> genes as molecular markers in support of subgroup identification of two 16SrXIII phytoplasmas associated with strawberry phyllody in Chile

Cui, Weier; Quiroga, Nicolás; Bertaccini, Assunta; Zamorano, Alan; Fiore, Nicola

doi:10.5958/2249-4677.2019.00045.8

Cited by 2 publications

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“…On the other hand, the phytoplasma 16SrXIII-F was detected for the first time in Chile in strawberry orchards of the Region of Valparaíso, associated with symptoms of phyllody and virescence [36]. Subsequent studies showed that this phytoplasma was present in all the strawberry production regions in Chile, including the localities where positive citrus samples have been detected in this survey [21,37]. In addition, this phytoplasma has been reported in calafate (Berberis microphylla G.…”

Section: Discussionmentioning

confidence: 78%

Survey for ‘Candidatus Liberibacter’ and ‘Candidatus Phytoplasma’ in Citrus in Chile

et al. 2021

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The considerable economic losses in citrus associated with ‘Candidatus Liberibacter’ and ‘Candidatus Phytoplasma’ presence have alerted all producing regions of the world. In Chile, none of these bacteria have been reported in citrus species. During the years 2017 and 2019, 258 samples presenting symptoms similar to those associated with the presence of these bacteria were examined. No detection of ‘Ca. Liberibacter’ associated with “huanglongbing” disease was obtained in the tested samples; therefore, this quarantine pest is maintained as absent in Chile. However, 14 plants resulted positive for phytoplasmas enclosed in subgroups 16SrV-A (12 plants) and 16SrXIII-F (2 plants). Although they have been found in other plant species, this is the first report of these phytoplasmas in citrus worldwide.

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Section: Discussionmentioning

confidence: 78%

Survey for ‘Candidatus Liberibacter’ and ‘Candidatus Phytoplasma’ in Citrus in Chile

et al. 2021

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“…Total nucleic acids were extracted from leaf midribs and insects using a CTAB method [31], dissolved in Tris-EDTA pH 8.0 buffer and maintained at 4 • C. PCR amplification was carried out using 20 ng µL −1 of nucleic acid. Direct and nested PCR assays on the 30 S ribosomal protein SSu12p gene were performed according to the protocol of Cui et al [32]. Further PCR with primer pair P1/P7 [33,34] and nested PCR with R16F2n/R2 primers amplifying the 16S rRNA gene [35] were performed as described by Schaff et al [36].…”

Section: Phytoplasma Detectionmentioning

confidence: 99%

Update and New Epidemiological Aspects about Grapevine Yellows in Chile

et al. 2020

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To date, phytoplasmas belonging to six ribosomal subgroups have been detected to infect grapevines in Chile in 36 percent of the sampled plants. A new survey on the presence of grapevine yellows was carried out from 2016 to 2020, and 330 grapevine plants from the most important wine regions of the country were sampled and analyzed by nested PCR/RFLP analyses. Phytoplasmas enclosed in subgroups 16SrIII-J and 16SrVII-A were identified with infection rates of 17% and 2%, respectively. The vineyards in which the phytoplasma-infected plants were detected were further inspected to identify alternative host plants and insects of potential epidemiological relevance. Five previously unreported plant species resulted positive for 16SrIII-J phytoplasma (Rosa spp., Brassica rapa, Erodium spp., Malva spp. and Rubus ulmifolius) and five insect species were fully or partially identified (Amplicephalus ornatus, A. pallidus, A. curtulus, Bergallia sp., Exitianus obscurinervis) as potential vectors of 16SrIII-J phytoplasmas. The 16SrVII-A phytoplasmas were not detected in non-grape plant species nor in insects. This work establishes updated guidelines for the study, management, and prevention of grapevine yellows in Chile, and in other grapevine growing regions of South America.

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Use of 12p and 36p genes as molecular markers in support of subgroup identification of two 16SrXIII phytoplasmas associated with strawberry phyllody in Chile

Cited by 2 publications

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Survey for ‘Candidatus Liberibacter’ and ‘Candidatus Phytoplasma’ in Citrus in Chile

Survey for ‘Candidatus Liberibacter’ and ‘Candidatus Phytoplasma’ in Citrus in Chile

Update and New Epidemiological Aspects about Grapevine Yellows in Chile

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