Use of Rhizobium leguminosarum as a potential biofertilizer for Lactuca sativa and Daucus carota crops

Abstract

Microbial biofertilizers are becoming an effective tool for sustainable agriculture by means of the reduction of the use of chemical fertilizers. However, the knowledge of each specific plant–microorganism interaction is essential for a correct application. In this study, we analyzed the in vitro plant‐growth‐promotion mechanisms of a Rhizobium leguminosarum strain named PEPV16 isolated from Phaseolus vulgaris nodules. This strain was able to produce siderophores and indole acetic acid and to solubilize phosphate. Confocal microscopy showed that this strain was able to colonize the roots of two horticultural crops, Lactuca sativa L. (lettuce) and Daucus carota L. (carrot). Strain PEPV16 was also able to promote the plant growth of both plant species increasing the dry matter of shoots and roots of lettuce and carrots, respectively, as well as to increase the uptake of N and P in the edible parts of both plant species. These data confirmed the suitability of Rhizobium as biofertilizer for nonlegumes.

Effective Colonization of Spinach Root Surface by Rhizobium

“…10.1d-f). Our results revealed that PEPV12 rhizobial strain inoculation presented the same distribution, which has been reported in many studies (Chabot et al 1996;García-Fraile et al 2012;Flores-Félix et al 2013). Furthermore, Perrine-Walker et al (2007) showed that Rhizobium strains form similar patterns in Oryza sativa roots and lateral roots than in spinach roots ( Fig.…”

“…Since some rhizobial strains were described as PGPR, there are a huge number of studies showing their abilities to increase legume and non-legume crops production (Antoun et al 1998;Alami et al 2000;Mishra et al 2006;García-Fraile et al 2012;Flores-Félix et al 2013). These studies analysed the ability and effect to establish communities and produce an important growth and effective colonization from rhizobial strains.…”

“…García-Fraile et al (2012) observed that two species of rhizobia firmly attached to tomato and pepper root surfaces, colonizing intercellular spaces. Moreover, Flores-Félix et al (2013) showed that a Rhizobium leguminosarum strain colonize root epidermis and intercellular spaces in Lactuca sativa and Daucus carota plants.…”

Role of plant growth promoting rhizobacteria in sustainable production of vegetables: Current perspective

“…Apart from free living PGPR, the microorganisms belonging to nitrogen fixing group for example Rhizobium, Mesorhizobium and Bradyrhizobium etc. have also been used to optimize vegetables productions (Antoun et al, 1998;Flores-Félix et al, 2013).…”

Interação entre isolados de rizóbios e genótipos de tomateiro (Solanum lycopersicum)

“…Dentre as plantas não-leguminosas em que houve comprovação de estímulos em seu crescimento por rizóbios estão, por exemplo, mostarda (Brassica campestris) (CHANDRA et al, 2007), alface (Lactuca sativa), cenoura (Daucus carota) (FLORES-FÉLIX et al, 2013), morango (Fragaria sp.) (FLORES-FÉLIX et al, 2015), abóbo-ra (Cucurbita moschata Duchesne ex Poir.)…”