Use of immunohistochemical analysis of CK5/6, CK14, and CK34betaE12 in the differential diagnosis of solid papillary carcinoma in situ from intraductal papilloma with usual ductal hyperplasia of the breast

Maeda, Ichiro; Tajima, Shinya; Kanemaki, Yoshihide; Tsugawa, Koichiro; Takagi, Masayuki

doi:10.1177/2050312118811542

Cited by 7 publications

(7 citation statements)

References 28 publications

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“…3% of malignant papillary breast lesions exhibited a positive expression. The findings of our investigation aligned with those of previous study (23) which demonstrated that increased positivity of CK14 in all cases intraductal papilloma while low to negligible expression was observed in all cases of solid papillary carcinoma in situ cases. The findings of previous investigation (24) corroborate with our own results, as CK 14 was found to be positively expressed in over 95% of papillomas but only in 12-20% of ductal carcinoma in situ associated with papillomas.…”

Section: Discussionsupporting

confidence: 92%

Diagnostic Significance Of Immunohistochemistry in Papillary Breast Neoplasms

Bassyoni,

Elshawarby,

Abostate

2023

Benha Medical Journal

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Background: Papillary breast neoplasms are heterogenous group of breast diseases consisting of wide range of benign, atypical and malignant lesions. Evaluation of such papillary neoplasms is considered one of the most problematic issues in diagnosis of breast pathology depending on H&E only as they need different interventional treatment modalities. Aim of study: To differentiate papillary breast lesions depending on immunohistochemical staining of P63, SMA, CK14, chromogranin and ER. Materials and Methods: An Immunohistochemical study of P63, SMA, CK14, chromogranin and ER were conducted on 75 cases of different papillary breast neoplasms; papilloma (20 cases ) , DCIS ( 25 cases ) , solid papillary carcinoma ( 12 cases ), intracystic papillary carcinoma ( 11cases) and invasive micropapillary carcinoma (7 cases) . Results: Out of 75 cases, all cases of duct papillomas were positive to SMA, P63. Ninety percent (90 %) of them were positive to CK14 in comparison to malignant papillary: DCIS, intracystic papillary carcinomas. solid papillary carcinomas neoplasms which were negative (84.0%, 92.3 %, 90.0% & 100.0%, respectively). Ninety -two percentage (92 %) of DCIS cases, 92.3% intracystic papillary carcinomas, 90.0% solid papillary carcinomas and all cases of invasive micropapillary carcinomas-showed homogenous positivity for ER compared to papillomas that showed focal heterogenous expression with statistical significant (p<0.01). Solid papillary carcinoma cases were diffusely positive for chromgranin. P63 was more specific than SMA and CK14 in diagnosis of ductal papilloma (100% Specificity). chromogranin was more specific in discriminating solid papillary carcinoma (95.0% Specificity) .ER was the most sensitive marker in diagnosis of malignant papillary lesions (92.7 % sensitivity). Conclusion: A combined panel of markers consisting of P63, SMA, CK14, chromogranin and ER-could be helpful in differentiating begin, atypical and malignant papillary breast lesions which need different interventional treatment modalities. A wider scale study on different variants of malignant papillary lesions is recommended.

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Section: Discussionsupporting

confidence: 92%

Diagnostic Significance Of Immunohistochemistry in Papillary Breast Neoplasms

Bassyoni,

Elshawarby,

Abostate

2023

Benha Medical Journal

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“…The correct diagnosis is facilitated by recognition of the uniform nature of the epithelial cells, the delicate fibrovascular network, and the absence of MECs within and usually at the periphery of the nodules. In difficult cases, IHC is helpful in demonstrating the absence of MECs and the clonal nature of the epithelial cell proliferation that is diffusely ER‐positive and HMW CK‐negative 72 …”

Section: Malignant Conditions That May Be Misinterpreted As Benignmentioning

confidence: 99%

“…In difficult cases, IHC is helpful in demonstrating the absence of MECs and the clonal nature of the epithelial cell proliferation that is diffusely ER‐positive and HMW CK‐negative. 72 …”

Section: Malignant Conditions That May Be Misinterpreted As Benignmentioning

confidence: 99%

Pitfalls in breast pathology

2022

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Accurate pathological diagnosis is the cornerstone of optimal clinical management for patients with breast disease. As non-operative diagnosis has now become the standard of care, histopathologists encounter the daily challenge of making definitive diagnoses on limited breast core needle biopsy (CNB) material. CNB samples are carefully evaluated using microscopic examination of haematoxylin and eosin (H&E)-stained slides and supportive immunohistochemistry (IHC), providing the necessary information to inform the next steps in the patient care pathway. Some entities may be difficult to distinguish on small tissue samples, and if there is uncertainty a diagnostic excision biopsy should be recommended. This review discusses (1) benign breast lesions that may mimic malignancy, (2) malignant conditions that may be misinterpreted as benign, (3) malignant conditions that may be incorrectly diagnosed as primary breast carcinoma, and (4) some IHC pitfalls. The aim of the review is to raise awareness of potential pitfalls in the interpretation of breast lesions that may lead to underdiagnosis, overdiagnosis, or incorrect classification of malignancy with potential adverse outcomes for individual patients.

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“…In tissues from patients with IPUDH, signicantly more cells were stained with CK34betaE12 than CK5/6 (p < 0.05) or CK14 (p < 0.05). 7 Fadare O et al (2007) found that there are 2 well-recognized cell populations lining the mammary duct system: the epithelial cells lining the lumen and the myoepithelial cells surrounding them. 5(13.9%) patient had HER2-positive (non-luminal), 1(2.8%) patient had Luminal A-like, 19(52.8%) patient had Luminal A-like, 3(8.3%) patient had luminal B-like HER 2+VE, 3(8.3%) patient had Luminal B-like( HER2-NEG) and 5(13.9%) patient had Triple-negative.There seems to be a phenotypic and biologic spectrum of basal-like or myoepithelial-type carcinomas, just as there is a wide range among tumors of luminal epithelial derivation/differentiation.…”

Section: Introductionmentioning

confidence: 99%

A Observational Study on Cytokeratin 5/6 Expression in Breast Carcinoma Patients and Correlated With Hormonal Status.

Ahmad

Imam²,

Jana

2020

ijsr

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INTRODUCTION Breast cancer is the second most common cause of morbidity and mortality worldwide in women. They vary remarkably in terms of clinical presentation, history, behaviour and genetic characteristics. There has been a significant increase in overall incidence of breast cancers especially so in the developing countries attributable mainly to globalization causing adaptation of western life style and improved access to diagnostic modalities. OBJECTIVE OF THE RESEARCH Study of cytokeratin 5/6 expression in proliferative, preinvasive and malignant lesions of breast. ER/PR expression in proliferative, preinvasive and malignant lesions of breast. Correlation of data using appropriate statistical methods. METHODOLOGY Patients attending the Dept of Pathology, Sri Krishna Medical Collage Muzaffarpur, Bihar with epithelial breast tumor. RESULT AND CONCLUSION We found that cytokeratin 5/6 expression in Breast Carcinoma was significantly associated with high grade of the tumour. cytokeratin 5/6 expression was significantly associated with advanced stage of the tumour. It was found that cytokeratin 5/6 expression was significantly correlated with ki67 in Breast Carcinoma patients. Hormonal receptor like ER and PR were was significantly associated with cytokeratin 5/6 expression tumour. Triple negative Breast Carcinoma patient was significantly associated with high grade of cytokeratin 5/6 expression tumour.

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Use of immunohistochemical analysis of CK5/6, CK14, and CK34betaE12 in the differential diagnosis of solid papillary carcinoma in situ from intraductal papilloma with usual ductal hyperplasia of the breast

Cited by 7 publications

References 28 publications

Diagnostic Significance Of Immunohistochemistry in Papillary Breast Neoplasms

Diagnostic Significance Of Immunohistochemistry in Papillary Breast Neoplasms

Pitfalls in breast pathology

A Observational Study on Cytokeratin 5/6 Expression in Breast Carcinoma Patients and Correlated With Hormonal Status.

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