Use of in vitro gas production models in ruminal kinetics

Pitt, R. E.; Cross, Timothy L.; Pell, Alice N.; Schofield, P; Doane, P.H.

doi:10.1016/s0025-5564(99)00020-6

Cited by 77 publications

(55 citation statements)

References 33 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…On the other hand, simple First Order Models that have been widely used in the kinetics of methane production can help in understanding the rates of digestion process [14]. This is not to mention that studies on the kinetics of methane production from the co-digestion of household agricultural wastes amongst themselves were not found in literature.…”

Section: Introductionmentioning

confidence: 99%

Effect of Variation in Co-Digestion Ratios of Matooke, Cassava and Sweet Potato Peels on Hydraulic Retention Time, Methane Yield and Its Kinetics

Tumutegyereize¹,

Ketlogetswe²,

Gandure³

et al. 2016

JSBS

View full text Add to dashboard Cite

This paper presents the results of batch anaerobic co-digestion of matooke, cassava, and sweet potato peels and vines. These agricultural wastes and others form the biggest portion of household wastes in developing countries. However, they have remained an unexploited resource amidst the ever increasing needs of clean energy and waste disposal challenges. Efforts to use them individually as biogas substrates have been associated with process acidification failure resulting from their fast hydrolysis. The aim of this work was to exploit agricultural wastes is co-digestion among themselves and assess their effect on methane yield and its kinetics, pH and hydraulic retention time (HRT). Sixteen ratios of Matooke peels (MP), cassava peels (CP) and sweet potato peels (SP) were assessed in duplicate. Methane yield and its kinetics, pH and HRT demonstrated dependence on the proportion of substrates in the mixture. Depending on the ratio mixture, HRT increased to 15 days compared to less than 5 days for single substrates, hydrolysis rate constant (k) reduced to a range of 0.1 -0.3 d −1 compared to single substrates whose k-values were above 0.5 d −1 , pH was maintained in the range of 6.38 -6.43 and CH 4 yield increased by 15% -200%. Ratios 2:1:0, 2:0:1, 0:1:2, 1:1:1 and 1:1:4 were consistent all through in terms of model fitting, having a positive synergetic effect on HRT, hydrolysis rate constant, lag phase and methane yield. However, more research is needed in maintaining the pH near the neutral for process stability assurance if household wastes are to be used as standalone substrates for biogas production without being co-substrates with livestock manure.

show abstract

Section: Introductionmentioning

confidence: 99%

Effect of Variation in Co-Digestion Ratios of Matooke, Cassava and Sweet Potato Peels on Hydraulic Retention Time, Methane Yield and Its Kinetics

Tumutegyereize¹,

Ketlogetswe²,

Gandure³

et al. 2016

JSBS

View full text Add to dashboard Cite

show abstract

“…All incubation procedures were repeated three times on days 1, 10, and 20. Gas volume data and incubation time were used to obtain the parameters of the kinetics of gas production: maximum volume of gas produced (Vm), lag phase (L), and rate of gas production (S), using the logistic model Vo = Vm / (1 + exp (2-4 * s * (t L) ) described by Pitt et al (1999). On incubation day 20, the volume and production of CO 2 after 24 h of fermentation were measured volumetrically, estimating the methane and minor gases by the difference in these values (Singh and Mohini, 1999).…”

Section: Methodsmentioning

confidence: 99%

Effect of calcium propionate and monensin on in vitro digestibility and gas production

et al. 2017

View full text Add to dashboard Cite

-An evaluation of the effects of monensin and calcium propionate on the in vitro kinetics of gas production, digestibility, carbon dioxide, and minor gas production on different days was performed using the ruminal fluid from eight Suffolk lamb donors, after receiving additives for 1, 10, and 20 days. Treatments consisted of a control ration of 40% grain; 30 mg/kg of monensin in a diet with 40% grain; 10 g/kg calcium propionate in a diet with 30% grain; and the combination of both additives in a diet with 30% grain. The gas production was measured up to 72 h of incubation and all incubation procedures were repeated three times on days 1, 10, and 20. On incubation day 20, the volume and production of methane and minor gases were measured. There was an interaction between calcium propionate and monensin for maximum gas production, in vitro dry matter digestibility (IVDMD), carbon dioxide, and minor gases. Monensin reduced gas production on days 1 and 20, whereas calcium propionate increased gas production (Vm) on day 1. The rate of gas production (s) was reduced by calcium propionate on day 1 and by the combination of additives on day 10. Lag time was reduced by monensin on day 10; however, it declined linearly with the feeding time of the additives. Monensin had no effect on IVDMD (62.29 vs. 62.24%), while calcium propionate increased the IVDMD (60.00 vs. 64.53%). The inclusion of monensin increased CO 2 ; however, the combination of monensin and calcium propionate had no effect on CO 2 production. Monensin reduced methane (25.37 vs. 20.29%) and increased CO 2 . None of the additives showed consistent effects on the kinetic parameters of in vitro gas production over time. The treatments with monensin and calcium propionate showed a significant reduction in methane production, with a higher fermentation efficiency since the IVDMD was increased. Both additives are a strategy to consider to reduce methane emissions without affecting the ruminal fermentation.

show abstract

“…The accumulated volume for each measured time was also measured and used to estimate the maximum volume (Mv; mL g -1 DM), the gas production rate (S; mL h -1 ) and the phase lag (L; h) of gas production, which were adjusted with the logistic model Vo = Mv/ (1 ? e (2-4 * S * (TL) ) (Pitt et al 1999). The in vitro dry matter digestibility (IVDMD) was calculated by the difference between the weight of the starting matter before and after incubation for 72 h of incubation, and the potential emission rate of fermentation gases (PERFG), was calculated by dividing the maximum volume of gas production from every cultivar divided by its in vitro dry matter digestibility value.…”

Section: Fermentation and In Vitro Digestibilitymentioning

confidence: 99%

Evaluation of eleven Mexican cultivars of prickly pear cactus trees for possibly utilization as animal fed: in vitro gas production

et al. 2016

View full text Add to dashboard Cite

In production systems of prickly pear fruit and prickly pear cactus, significant amounts of pruned material, which could be used as an ingredient in animal feeding, is generated. The aim of this study was to measure the nutrient content, fermentation kinetics and in vitro digestibility of eleven cultivars of cladodes of prickly pear cactus. The fermentation was measured indirectly using the gas production technique, where 500 mg of DM substrate (prickly pear cactus cultivars) were placed in amber glass flasks of 125 mL with 90 mL of ruminal inoculum under a continuous flow of CO 2 . Flasks were hermetically wrapped up with a rubber stopper and a metal ring, and were placed in a water bath at 39°C. The fermentation gas pressure was measured using a monometer at 0, 2, 4, 6, 8, 12, 16, 20, 24, 32, 38, 50 and 72 h incubation. Results show that the chemical composition ranged between (g kg -1 DM): 64 and 160 g kg -1 dry matter (DM), 137-293 g kg -1 DM ash, 52-101 g kg -1 DM crude protein, 3-22 g kg -1 DM ether extract, 251-393 neutral detergent fiber and 132-192 acid detergent fiber. The gas volume was different (p \ 0.05) among cultivars, with the Red Vigor cultivar having the largest volume at 378 mL g -1 DM. The highest volume of gas was produced during the first 24 h in on average 67 % of the total produced. The in vitro DM digestibility displayed values of up to 82 % in the Roja Pelota cultivar. It can be concluded that the cladodes of prickly pear cactus from different cultivars can be used in animal feed for its good rumen fermentation characteristics.

show abstract

Use of in vitro gas production models in ruminal kinetics

Cited by 77 publications

References 33 publications

Effect of Variation in Co-Digestion Ratios of Matooke, Cassava and Sweet Potato Peels on Hydraulic Retention Time, Methane Yield and Its Kinetics

Effect of Variation in Co-Digestion Ratios of Matooke, Cassava and Sweet Potato Peels on Hydraulic Retention Time, Methane Yield and Its Kinetics

Effect of calcium propionate and monensin on in vitro digestibility and gas production

Evaluation of eleven Mexican cultivars of prickly pear cactus trees for possibly utilization as animal fed: in vitro gas production

Contact Info

Product

Resources

About