Use of inverse polymerase chain reaction to characterize a novel human herpesvirus 7 isolate

Poirel, Laurent; Aubin, Jean-Thierry; Gautheret, A.; Malet, Isabelle; Huraux, Jean‐Marie; Agut, Henri

doi:10.1016/s0166-0934(96)02167-2

Cited by 10 publications

(8 citation statements)

References 17 publications

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“…After sequencing of the PCR products, 580-bp-long sequences were characterized. In order to obtain the entire sequences of the ␤-lactamase genes, the inverse PCR (IPCR) technique was used (20). This technique enabled us to amplify, by PCR, flanking regions of a DNA fragment.…”

Section: Methodsmentioning

confidence: 99%

Genetic Diversity of Carbapenem-Hydrolyzing Metallo-β-Lactamases from Chryseobacterium ( Flavobacterium ) indologenes

Bellais

Poirel

Léotard

et al. 2000

Antimicrob Agents Chemother

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The genus Chryseobacterium, defined in 1994 by Vandamme et al., comprises six species, including Chryseobacterium meningosepticum (previously Flavobacterium meningosepticum) and Chryseobacterium indologenes (previously Flavobacterium indologenes), which are the most common clinical species of this genus (29). C. meningosepticum isolates are associated with meningitis in newborns or in immunocompromised patients (4). C. indologenes is responsible mostly for nosocomial infections linked to the use of intravascular devices (10, 28). In 1997, Hsueh et al. reported 36 cases of infections caused by C. indologenes over a 3-year period at the National Taiwan University Hospital (11). These infections were intra-abdominal infections, biliary tract infections, or wound sepsis (11). C. indologenes is also implicated in pneumonia (9). C. indologenes is resistant to nearly all penicillins, except piperacillin, to restricted-spectrum cephalosporins, to aztreonam, and to carbapenems (1). Among ␤-lactams, only extended-spectrum cephalosporins show in vitro activity against C. indologenes (8). Minocycline and ciprofloxacin seem to be the most effective antibiotics and have been successfully used to treat C. indologenes infections (10).Recently, two Ambler class B ␤-lactamases have been characterized for C. meningosepticum, which is also resistant to all ␤-lactams, including carbapenems (2, 26). The unrelated BlaB and GOB-1 exhibit a broad-spectrum profile and are chromosome encoded. These carbapenem-hydrolyzing ␤-lactamases (CH␤Ls) have molecular and biochemical heterogeneity among C. meningosepticum isolates. We have characterized a ␤-lactamase, IND-1, from C. indologenes strain 001 (1). Since plasmid-mediated CH␤Ls have been increasingly reported for gram-negative species worldwide (5,12,18,23,24,25), it was of interest to identify naturally occurring CH␤Ls of gramnegative species and to search for their degree of identity with the plasmid-mediated CH␤Ls. Thus, we performed an extended genetic and biochemical study of ␤-lactamase-mediated resistance to carbapenems in C. indologenes. MATERIALS AND METHODSBacterial strains. The bacterial strains used in this work are listed in Table 1. C. indologenes 002, 007, 008, and 009 were isolated from bronchoalveolar brush border, blood culture, rectal swab, and biliary liquid drainage, respectively, at the Hôpital de Bicêtre (Le Kremlin-Bicêtre, France) from 1997 to 1999. C. indologenes 003 and 004 were isolated from blood cultures at the Hôpital Robert Debré (Paris, France). C. indologenes 005 was from the Assistance Publique-Hôpitaux de Marseille (Marseille, France). C. indologenes 006 was isolated at the Hôpital de la Pitié Salpêtrière (Paris, France), and the C. indologenes reference strain CIP101026 was from the Pasteur Institute (Paris, France) strain collection. Each C. indologenes isolate was identified according to standard biochemical techniques (19,29,31), and their identification was checked at the Center for Bacterial Identification at the Pasteur Institute. These C. indolog...

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Section: Methodsmentioning

confidence: 99%

Genetic Diversity of Carbapenem-Hydrolyzing Metallo-β-Lactamases from Chryseobacterium ( Flavobacterium ) indologenes

Bellais

Poirel

Léotard

et al. 2000

Antimicrob Agents Chemother

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“…Human herpesvirus 7 (HHV-7) was originally isolated from the stimulated CD4 ϩ T cells of a healthy individual (9) and was subsequently characterized as a ubiquitous virus, infecting most human beings (5,10,17,25,27). This virus was classified in the Betaherpesvirinae subfamily on the basis of its genetic organization (3,6,15,16).…”

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confidence: 99%

“…Only seven of these combinations (Co) were observed, and 75% of the samples corresponded to two of them, designated provisionally Co1 and Co2. The reference strain JI was defined as Co2, as were our reference isolate, IM (17), and the original HHV-7-positive sample which was the source of IM. The reference strain RK corresponded to an eighth combination (Co8) which was not observed in the group of human samples we investigated.…”

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confidence: 99%

Preferential Associations of Alleles of Three Distinct Genes Argue for the Existence of Two Prototype Variants of Human Herpesvirus 7

Franti

Aubin

Gautheret‐Dejean

et al. 1999

J Virol

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We had previously described six distinct alleles of the glycoprotein B (gB) gene of human herpesvirus 7 (HHV-7). The genetic changes corresponding to these alleles did not affect gB gene transcription or translation in in vitro assays. The study of distinct HHV-7-positive human samples showed preferential associations of some gB alleles with some alleles of two other genes, distantly located on the HHV-7 genome, coding for the phosphoprotein p100 (p100) and the major capsid protein (MCP). Two allele combinations, corresponding to 44 and 31% of the samples studied, respectively, were interpreted as the genetic signatures of two major prototype HHV-7 variants.

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“…HHV-6 variant A strain TAN and HHV-6 variant B strain MAR were obtained by coculture of peripheral blood mononuclear cells (PBMCs) from healthy blood donors (2). HHV-7 IM was obtained in our laboratory by culture of saliva from a healthy subject on PBMCs from healthy blood donors (19). Amplification of betaglobin gene sequences was performed with the primers previously described (21) to test for the presence of nucleated cells and to verify the absence of major Taq polymerase inhibitors.…”

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confidence: 99%

Detection of human Betaherpesvirinae in saliva and urine from immunocompromised and immunocompetent subjects

et al. 1997

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Human cytomegalovirus (HCMV) is a well-known opportunistic agent that reactivates in human immunodeficiency virus (HIV)-seropositive subjects. Human herpesvirus 6 (HHV-6) and HHV-7 were discovered recently and, like HCMV, belong to the Betaherpesvirinae subfamily. We looked for the presence of HCMV, HHV-6, and HHV-7 by PCR with saliva and urine samples from 125 HIV-seropositive patients at different stages of HIV infection and with saliva and urine samples from 29 HIV-seronegative subjects. All three viruses were frequently detected in the saliva (overall rates of detection, 61, 43, and 63% for HCMV, HHV-6, and HHV-7, respectively) with no correlation with the stage of immune deficiency. In contrast, HCMV was detected in urine much more frequently than the two other herpesviruses (overall rates of detection, 37, 2, and 6.5% for HCMV, HHV-6, and HHV-7, respectively) and was associated with immune deficiency. This suggests that these three genetically related viruses differ from each other with regard to replication in the urinary tract.

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Use of inverse polymerase chain reaction to characterize a novel human herpesvirus 7 isolate

Cited by 10 publications

References 17 publications

Genetic Diversity of Carbapenem-Hydrolyzing Metallo-β-Lactamases from Chryseobacterium ( Flavobacterium ) indologenes

Genetic Diversity of Carbapenem-Hydrolyzing Metallo-β-Lactamases from Chryseobacterium ( Flavobacterium ) indologenes

Preferential Associations of Alleles of Three Distinct Genes Argue for the Existence of Two Prototype Variants of Human Herpesvirus 7

Detection of human Betaherpesvirinae in saliva and urine from immunocompromised and immunocompetent subjects

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