Use of Kova-Slide II with Grid and Uncentrifuged Segmented Urine Specimens in the Diagnosis of Nongonococcal Urethritis

Perera, Shanthan A.B.

doi:10.1097/00007435-198501000-00004

Cited by 10 publications

(3 citation statements)

References 8 publications

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“…10 Swabs were also examined microscopically to provide presumptive diagnosis for the presence of Neisseria gonorrhoeae infection (i.e. 10 Swabs were also examined microscopically to provide presumptive diagnosis for the presence of Neisseria gonorrhoeae infection (i.e.…”

Section: Methodsmentioning

confidence: 99%

“…Urethral swabs were obtained from males reporting dysuria and/or urethral discharge, and these smears were Gram-stained and microscopically assessed for NGU, which was defined as five or more polymorphonuclear leukocytes on average over 5 high-powered fields (¥100 oil objective). 10 Swabs were also examined microscopically to provide presumptive diagnosis for the presence of Neisseria gonorrhoeae infection (i.e. gonococcal urethritis).…”

Section: Methodsmentioning

confidence: 99%

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Mycoplasma genitalium: prevalence in men presenting with urethritis to a South Australian public sexual health clinic

Mezzini

Waddell

Douglas

et al. 2013

Internal Medicine Journal

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Section: Methodsmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

Mycoplasma genitalium: prevalence in men presenting with urethritis to a South Australian public sexual health clinic

Mezzini

Waddell

Douglas

et al. 2013

Internal Medicine Journal

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“…Participants provided an MSU specimen that was immediately divided into four equal aliquots. Aliquot one was analyzed directly for pyuria (white cell count ≥10 3 CFU/ml) using microscopy within one hour of collection in a KOVA slide hemocytometer ( Perera, 1985 ; Emerson and Emerson, 2005 ). Enhanced culture was carried out by inoculating 0.001 ml of urine onto chromogenic (trypticase soy agar with 5% sheep blood), chocolate, and fastidious anaerobic agar (FAA) culture plates within one hour of sample collection.…”

Section: Patients/subjects Materials and Methodsmentioning

confidence: 99%

Chronic Urinary Infection in Overactive Bladder Syndrome: A Prospective, Blinded Case Control Study

Khan

Healey

Paravati

et al. 2021

Front. Cell. Infect. Microbiol.

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ObjectivesTo investigate whether women with overactive bladder (OAB) symptoms and no evidence of clinical infection by conventional clean-catch midstream urine cultures have alternative indicators of sub-clinical infection.Patients/Subjects, Materials & MethodsThe study was a prospective, blinded case-control study with 147 participants recruited, including 73 OAB patients and 74 controls. The OAB group comprised female patients of at least 18 years of age who presented with OAB symptoms for more than 3 months. Clean-catch midstream urine samples were examined for pyuria by microscopy; subjected to routine and enhanced microbiological cultures and examined for the presence of 10 different cytokines, chemokines, and prostaglandins by ELISA.ResultsThe mean age and BMI of participants in both groups were similar. No significant difference in the number of women with pyuria was observed between OAB and control groups (p = 0.651). Routine laboratory cultures were positive in three (4%) of women in the OAB group, whereas the enhanced cultures isolated bacteria in 17 (23.2%) of the OAB patients. In the control group, no positive cultures were observed using routine laboratory cultures, whereas enhanced culture isolated bacteria in 8 (10.8%) patients. No significant differences were observed in the concentrations of PGE2, PGF2α, MCP-1, sCD40L, MIP-1β, IL12p70/p40, IL12/IL-23p40, IL-5, EGF and GRO-α between the OAB and control groups.ConclusionsPatients with OAB symptoms have significant bacterial growth on enhanced culture of the urine, which is often not detectable through routine culture, suggesting a subclinical infection. Enhanced culture techniques should therefore be used routinely for the effective diagnosis and management of OAB.

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Preventive lymecycline therapy in women with a history of pelvic inflammatory disease undergoing first-trimester abortion: a clinical, controlled trial

Heisterberg

Gnarpe

1988

European Journal of Obstetrics & Gynecology and Reproductiv

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Use of Kova-Slide II with Grid and Uncentrifuged Segmented Urine Specimens in the Diagnosis of Nongonococcal Urethritis

Cited by 10 publications

References 8 publications

Mycoplasma genitalium: prevalence in men presenting with urethritis to a South Australian public sexual health clinic

Mycoplasma genitalium: prevalence in men presenting with urethritis to a South Australian public sexual health clinic

Chronic Urinary Infection in Overactive Bladder Syndrome: A Prospective, Blinded Case Control Study

Preventive lymecycline therapy in women with a history of pelvic inflammatory disease undergoing first-trimester abortion: a clinical, controlled trial

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