Use of liposomal amplifiers in total internal reflection fluorescence fiber-optic biosensors for protein detection

Chang, Yu Lin; Fu, Chien Chung; Chen, Yi Ting; Jou, Amily Fang-Ju; Chen, Chii Chang; Chou, Chien; Ho, Ja‐an Annie

doi:10.1016/j.bios.2015.10.012

Cited by 22 publications

(11 citation statements)

References 42 publications

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“…The detection time was just one hour and the sensor showed a 36-fold sensitivity improvement upon silver enhancement (Figure 8d). Although such an assembly has been employed for the detection of immunoglobulin [170], plastic optical fibre-based sensors in conjunction with liposomal amplifiers show 10 6 times higher sensitivity [172]. Such plastic optical fibres have also enabled rapid pathogen detection in 30 minutes over a wide concentration range (10 4 -10 8 MPN mL −1 ) [173].…”

Section: Evanescent Wave-based Sensors For Biomedical Diagnosticsmentioning

confidence: 99%

Recent Progress in Optical Sensors for Biomedical Diagnostics

Pirzada

Altıntaş

2020

Micromachines

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In recent years, several types of optical sensors have been probed for their aptitude in healthcare biosensing, making their applications in biomedical diagnostics a rapidly evolving subject. Optical sensors show versatility amongst different receptor types and even permit the integration of different detection mechanisms. Such conjugated sensing platforms facilitate the exploitation of their neoteric synergistic characteristics for sensor fabrication. This paper covers nearly 250 research articles since 2016 representing the emerging interest in rapid, reproducible and ultrasensitive assays in clinical analysis. Therefore, we present an elaborate review of biomedical diagnostics with the help of optical sensors working on varied principles such as surface plasmon resonance, localised surface plasmon resonance, evanescent wave fluorescence, bioluminescence and several others. These sensors are capable of investigating toxins, proteins, pathogens, disease biomarkers and whole cells in varied sensing media ranging from water to buffer to more complex environments such as serum, blood or urine. Hence, the recent trends discussed in this review hold enormous potential for the widespread use of optical sensors in early-stage disease prediction and point-of-care testing devices.

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Section: Evanescent Wave-based Sensors For Biomedical Diagnosticsmentioning

confidence: 99%

Recent Progress in Optical Sensors for Biomedical Diagnostics

Pirzada

Altıntaş

2020

Micromachines

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“…To validate the performance of our biosensing system, liposomal samples were prepared from a lipid mixture using the reversed-phase evaporation method as reported previously. 33 In short, a lipid mixture that consisted of phosphatidylcholine (PC), phosphatidylglycerol (PG), and cholesterol (7 mg:0.5 mg:0.8/2.2/4 mg) was dissolved in a 2-mL mixture of chloroform, isopropyl ether, and methanol (6:6:1 volume ratio). After sonication of the mixture under nitrogen for 1 min, 1 mL PBS (10 mM, pH 7.4) was added with subsequent sonication under nitrogen for another 3 min.…”

Section: Real Sample Analysismentioning

confidence: 99%

Simple and Cost-effective Enzymatic Detection of Cholesterol Using Flow Injection Analysis

et al. 2020

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A flow-injection analytical (FIA) system was developed for the determination of cholesterol concentrations based on enzymatic reactions that occurred in a cholesterol oxidase (CHOx)-immobilized, fused-silica capillary followed by electrochemical detection. The production of hydrogen peroxide from cholesterol in an enzymatic reaction catalyzed by CHOx was subsequently oxidized electrochemically at an electrode. Our FlA system demonstrated its cost-effectiveness and utility at an applied potential of 0.6 V (vs. Ag/AgCl), a flow rate of 100 μL/min and, under optimal conditions, the resulting signal demonstrated a linear dynamic range from 50 μM to 1.0 mM with a limit of detection (LOD) of 12.4 μM, limit of quantification (LOQ) of 44.9 μM, and the coefficient of variation of 5.17%. In addition, validation of our proposed system using a reference HDL-cholesterol kit used for clinical diagnosis suggested our FIA system was comparable to commercial kits for the determination of the cholesterol incorporation amount in various aqueous liposomal suspensions. These good analytical features achieved by FIA could make the implementation of this methodology possible for on-line monitoring of cholesterol in various types of samples.

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“…The versatility of biosensors is also illustrated by the large number of targets they can detect, including bacteria [ 5 , 9 ], viruses [ 10 ] and a variety of biomolecules such as specific DNA sequences [ 11 ], proteins [ 12 , 13 ] and peptides [ 14 ].…”

Section: The Burden and Risks Of Bacterial Infectionsmentioning

confidence: 99%

Antimicrobial Peptides: Powerful Biorecognition Elements to Detect Bacteria in Biosensing Technologies

2018

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Bacterial infections represent a serious threat in modern medicine. In particular, biofilm treatment in clinical settings is challenging, as biofilms are very resistant to conventional antibiotic therapy and may spread infecting other tissues. To address this problem, biosensing technologies are emerging as a powerful solution to detect and identify bacterial pathogens at the very early stages of the infection, thus allowing rapid and effective treatments before biofilms are formed. Biosensors typically consist of two main parts, a biorecognition moiety that interacts with the target (i.e., bacteria) and a platform that transduces such interaction into a measurable signal. This review will focus on the development of impedimetric biosensors using antimicrobial peptides (AMPs) as biorecognition elements. AMPs belong to the innate immune system of living organisms and are very effective in interacting with bacterial membranes. They offer unique advantages compared to other classical bioreceptor molecules such as enzymes or antibodies. Moreover, impedance-based sensors allow the development of label-free, rapid, sensitive, specific and cost-effective sensing platforms. In summary, AMPs and impedimetric transducers combine excellent properties to produce robust biosensors for the early detection of bacterial infections.

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Use of liposomal amplifiers in total internal reflection fluorescence fiber-optic biosensors for protein detection

Cited by 22 publications

References 42 publications

Recent Progress in Optical Sensors for Biomedical Diagnostics

Recent Progress in Optical Sensors for Biomedical Diagnostics

Simple and Cost-effective Enzymatic Detection of Cholesterol Using Flow Injection Analysis

Antimicrobial Peptides: Powerful Biorecognition Elements to Detect Bacteria in Biosensing Technologies

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