Use of metal complexation in non-aqueous capillary electrophoresis systems for the separation and improved detection of tetracyclines

Tjørnelund, Jette; Hansen, Steen Honoré

doi:10.1016/s0021-9673(97)00421-4

Cited by 66 publications

(33 citation statements)

References 26 publications

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“…Solid-phase extraction (SPE) using the reversedphase octadecyl (C18) cartridge has become routine in purifying and concentrating environmental contaminants (20). A great number of methods for extracting tetracyclines using C18 have been developed to replace more labour-intensive liquid-liquid extraction (18) and lyophilisation (11).…”

Section: Discussionmentioning

confidence: 99%

Screening method for the determination of selected tetracyclines in water by liquid chromatography with diode array detector

Patyra

Kowalczyk

Kwiatek

2014

Bulletin of the Veterinary Institute in Pulawy

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A chromatographic procedure for determination of oxytetracycline (OXT), tetracycline (TC), chlorotetracycline (CTC), and doxycycline (DC) in water samples was developed and was applied for the analysis of water samples collected from poultry and pig farms and environmental water samples. Samples were acidified with trifluoroacetetic acid to pH 3 and further purified by solid phase extraction using Oasis HLB cartridges. The samples were dried up and redissolved in the mixture of oxalic acid and methanol. Separation was performed on reserved phase column (Phenomenex column C 18 , 250 mm × 4.6 mm, 5 µm) by multistep gradient elution, and detection was carried out at 360 nm for OTC and TC, 370 nm for CTC, and 350 nm for DC. The tetracyclines were eluted with the mobile phase of 0.05 M oxalic acid (pH 2.5), acetonitrile, and methanol. This method provided average recoveries of 83.53% to 108.59%, with coefficient of variations (CVs) of 2.41% to 8.64% in the range of 10 to 1000 µg/L OTC, TC, CTC, and DC in water. The linearity for the tetracyclines was determined by HPLC-DAD in the range 10 to 1000 µg/L, with the correlation coefficient (R) > 0.99. The LOD and LOQ for the tetracyclines in water samples ranged from 1.51 to 4.00 and 2.51 to 5.93 µg/L, respectively.

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Section: Discussionmentioning

confidence: 99%

Screening method for the determination of selected tetracyclines in water by liquid chromatography with diode array detector

Patyra

Kowalczyk

Kwiatek

2014

Bulletin of the Veterinary Institute in Pulawy

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“…Microbiological assay [2] once used is of less sensitivity and specificity. Thin layer chromatography [3], capillary electrophoresis [4,5] and high-performance liquid chromatography (HPLC) methods were used to determine antibiotics too. Of all these methods, HPLC coupled with different detection techniques such as UV or DAD [6][7][8][9], chemilumi-nescence [10][11][12], MS [13][14][15] and electrochemistry [1,[16][17][18][19][20][21][22] is the most common method to be used for the analysis of TCs.…”

Section: Introductionmentioning

confidence: 99%

Optimizing the integrated pulsed amperometric multicycle step waveform for the determination of tetracyclines

Cai

Shi

et al. 2006

Journal of Chromatography A

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A method of modified integrated pulsed amperometric detection with multicycle step waveform (Multi-IPAD) following high-performance liquid chromatography (HPLC) was applied for the determination of tetracyclines (TCs) including dimethyltetracycline (DMTC), oxytetracycline (OTC) and tetracycline (TC). The key advantages of the Multi-IPAD are the abilities to enhance sensitivity and reproducibility and the ability to keep working electrode clean through the use of a high-frequent waveform alteration in integration step and the use of a cleaning potential, which is quite different from conventional three-step potential waveform. The analyses were carried out using the mobile phase of acetonitrile-water mixture solution (10:90, v/v) containing 1% perchloric acid on a C 18 column at a flow rate of 0.21 mL/min. The IPAD waveform parameters were optimized to maximize the signal-to-noise ratio (S/N) and successfully applied for the sensitive detection of TCs. The detection limits (S/N = 3, 20 L injected) were 0.07 mg/L for DMTC, 0.08 mg/L for OTC and 0.05 mg/L for TC. The peak height relative standard deviations (RSDs) of every compound for replicate injection (n = 15) determined were below 4.6%.

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“…Several analytical methods have been reported for the determination of tetracylines in various samples, such as milk, [1][2][3][6][7][8][9] shrimp, 1,4 animal feeds, 5,10 animal tissues [11][12][13] and pharmaceutical formulations 14,15 based on thin-layer chromatography, 10 capillary electrophoresis, 16,17 and high performance liquid chromatography (HPLC). [6][7][8][11][12][13][14] HPLC is a common method that separates tetracyclines in the reversephase mode with different detection methods, such as spectrophotometry, 12,13 fluorometry, 18,19 mass spectrometry, 20,21 and electrochemistry.…”

Section: Introductionmentioning

confidence: 99%

Analysis of Tetracycline Antibiotics Using HPLC with Pulsed Amperometric Detection

et al. 2005

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Use of metal complexation in non-aqueous capillary electrophoresis systems for the separation and improved detection of tetracyclines

Cited by 66 publications

References 26 publications

Screening method for the determination of selected tetracyclines in water by liquid chromatography with diode array detector

Screening method for the determination of selected tetracyclines in water by liquid chromatography with diode array detector

Optimizing the integrated pulsed amperometric multicycle step waveform for the determination of tetracyclines

Analysis of Tetracycline Antibiotics Using HPLC with Pulsed Amperometric Detection

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