Use of Mitochondrial Cytochrome Oxidase I Polymerase Chain Reaction-Restriction Fragment Length Polymorphism for Identifying Subclades of Bemisia tabaci Mediterranean Group

Chu, Dong; Hu, Xiang-Shun; Gao, C.S.; Zhao, Hongwei; Nichols, Robert L.; Li, Xianchun

doi:10.1603/ec11039

Cited by 43 publications

(39 citation statements)

References 33 publications

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“…These differences are driven by the geographic differences in the composition of Bemisia endosymbiont communities (Gottlieb et al 2010), and insecticide-driven shifts in community structure may also alter vector competence. Recent research has also identified five well-defined MED haplotypes (Chu et al 2012a) whose endosymbiont communities may well differ. Exploring the interaction between insecticide resistance and endosymbiont communities may help develop management programs aimed at reducing viral outbreaks.…”

Section: Discussionmentioning

confidence: 99%

Insecticides promote viral outbreaks by altering herbivore competition

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Chu

et al. 2015

Ecological Applications

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Abstract. While the management of biological invasions is often characterized by a series of single-species decisions, invasive species exist within larger food webs. These biotic interactions can alter the impact of control/eradication programs and may cause suppression efforts to inadvertently facilitate invasion spread and impact. We document the rapid replacement of the invasive Bemisia Middle East-Asia Minor I (MEAM1) cryptic biotype by the cryptic Mediterranean (MED) biotype throughout China and demonstrate that MED is more tolerant of insecticides and a better vector of tomato yellow leaf curl virus (TYLCV) than MEAM1. While MEAM1 usually excludes MED under natural conditions, insecticide application reverses the MEAM1-MED competitive hierarchy and allows MED to exclude MEAM1. The insecticide-mediated success of MED has led to TYLCV outbreaks throughout China. Our work strongly supports the hypothesis that insecticide use in China reverses the MEAM1-MED competitive hierarchy and allows MED to displace MEAM1 in managed landscapes. By promoting the dominance of a Bemisia species that is a competent viral vector, insecticides thus increase the spread and impact of TYLCV in heterogeneous agroecosystems.

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Section: Discussionmentioning

confidence: 99%

Insecticides promote viral outbreaks by altering herbivore competition

Pan

Preisser

Chu

et al. 2015

Ecological Applications

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“…tabaci . The primers used for detection of the species were C1-J-2195 (5′-TTGATTTTTTGGTCATCCAGAAGT-3′) [28] and R-BQ-2819 (5′-CTGAATATCGRCGAGGCATTCC −3′) [29]. All PCR reactions were performed in 20 µl buffer containing 2 µl 10× buffer, 1.5 mM MgCl 2 , 0.2 µM of each primer, 0.2 µM dNTPs, 1 unit Taq DNA polymerase, and 2 µl template DNA.…”

Section: Methodsmentioning

confidence: 99%

Competitive Ability and Fitness Differences between Two Introduced Populations of the Invasive Whitefly Bemisia tabaci Q in China

et al. 2014

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BackgroundOur long-term field survey revealed that the Cardinium infection rate in Bemisia tabaci Q (also known as biotype Q) population was low in Shandong, China over the past few years. We hypothesize that (1) the Cardinium-infected (C +) B. tabaci Q population cannot efficiently compete with the Cardinium-uninfected (C −) B. tabaci Q population; (2) no reproductive isolation may have occurred between C + and C −; and (3) the C − population has higher fitness than the C + population.Methodology and ResultsTo reveal the differences in competitive ability and fitness between the two introduced populations (C + and C −), competition between C + and C − was examined over several generations. Subsequently, the reproductive isolation between C + and C − was studied by crossing C + with C − individuals, and the fitnesses of C + and C − populations were compared using a two-sex life table method. Our results demonstrate that the competitive ability of the C + whiteflies was weaker than that of C −. There is that no reproductive isolation occurred between the two populations and the C − population had higher fitness than the C + population.ConclusionThe competitive ability and fitness differences of two populations may explain why C − whitefly populations have been dominant during the past few years in Shandong, China. However, the potential role Cardinium plays in whitefly should be further explored.

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“…Results of mtCOI gene PCR-RFLP assays [12] and direct DNA sequencing followed by phylogenetic evaluation against reference sequences [13] both confirmed that the Bemisia in the MED/Q colony belonged to the Q1 haplotype group, or western Mediterranean region clade (data not shown).…”

Section: Introductionmentioning

confidence: 99%

Genome sequencing of the sweetpotato whitefly Bemisia tabaci MED/Q

et al. 2017

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The sweetpotato whitefly Bemisia tabaci is a highly destructive agricultural and ornamental crop pest. It damages host plants through both phloem feeding and vectoring plant pathogens. Introductions of B. tabaci are difficult to quarantine and eradicate because of its high reproductive rates, broad host plant range, and insecticide resistance. A total of 791 Gb of raw DNA sequence from whole genome shotgun sequencing, and 13 BAC pooling libraries were generated by Illumina sequencing using different combinations of mate-pair and pair-end libraries. Assembly gave a final genome with a scaffold N50 of 437 kb, and a total length of 658 Mb. Annotation of repetitive elements and coding regions resulted in 265.0 Mb TEs (40.3%) and 20 786 protein-coding genes with putative gene family expansions, respectively. Phylogenetic analysis based on orthologs across 14 arthropod taxa suggested that MED/Q is clustered into a hemipteran clade containing A. pisum and is a sister lineage to a clade containing both R. prolixus and N. lugens. Genome completeness, as estimated using the CEGMA and Benchmarking Universal Single-Copy Orthologs pipelines, reached 96% and 79%. These MED/Q genomic resources lay a foundation for future ‘pan-genomic’ comparisons of invasive vs. noninvasive, invasive vs. invasive, and native vs. exotic Bemisia, which, in return, will open up new avenues of investigation into whitefly biology, evolution, and management.

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Use of Mitochondrial Cytochrome Oxidase I Polymerase Chain Reaction-Restriction Fragment Length Polymorphism for Identifying Subclades of Bemisia tabaci Mediterranean Group

Cited by 43 publications

References 33 publications

Insecticides promote viral outbreaks by altering herbivore competition

Insecticides promote viral outbreaks by altering herbivore competition

Competitive Ability and Fitness Differences between Two Introduced Populations of the Invasive Whitefly Bemisia tabaci Q in China

Genome sequencing of the sweetpotato whitefly Bemisia tabaci MED/Q

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