2002
DOI: 10.1651/0278-0372(2002)022[0337:uomatc]2.0.co;2
|View full text |Cite
|
Sign up to set email alerts
|

Use of Monoclonal Antibodies to Classify Hemocyte Subpopulations of Tiger Shrimp (Penaeus Monodon)

Help me understand this report

Search citation statements

Order By: Relevance

Paper Sections

Select...
3
1
1

Citation Types

0
23
0
1

Year Published

2003
2003
2019
2019

Publication Types

Select...
6

Relationship

0
6

Authors

Journals

citations
Cited by 21 publications
(24 citation statements)
references
References 33 publications
0
23
0
1
Order By: Relevance
“…The difficulty of haemocyte classification may be due to the complexity of haemocyte sub-populations. In the three sub-populations (H, SG and G), there were at least two haemocyte subsets in SG and G identified by monoclonal antibodies Z6A5 and Z6H8 [21]. Most of the monoclonal antibodies obtained in this study recognized different ratios of H, SG and G sub-populations.…”
Section: Discussionmentioning
confidence: 61%
“…The difficulty of haemocyte classification may be due to the complexity of haemocyte sub-populations. In the three sub-populations (H, SG and G), there were at least two haemocyte subsets in SG and G identified by monoclonal antibodies Z6A5 and Z6H8 [21]. Most of the monoclonal antibodies obtained in this study recognized different ratios of H, SG and G sub-populations.…”
Section: Discussionmentioning
confidence: 61%
“…In Crustacea, THC, phenoloxidase activity, respiratory burst (release of superoxide anion), antioxidant enzyme activity (superoxide dismutase activity in particular), phagocytic activity and bacterial clearance efficiency are commonly considered as useful indicators of immunosurveillance status in animals [4]. In most Crustacea, THC and differential haemocytes count (DHC) vary markedly among individuals [14,31]. Results obtained in the present study showed that also in C. aestuarii a great inter-individual variability in circulating haemocyte number occurred, and the resulting mean haemocyte number was 6.4 (x10 6 )/mL haemolymph.…”
Section: Discussionmentioning
confidence: 99%
“…In Macrobrachium rosenbergii, Macrobrachium acanthurus and Penaeus monodon, Gargioni and Barracco [13] observed that semigranulocytes and granulocytes, but not hyalinocytes, phagocytised yeast particles in vitro. Conversely, a recent study demonstrated that hyalinocytes from P. monodon were the only haemocyte type involved in in vitro latex beads phagocytosis [14].…”
Section: Introductionmentioning
confidence: 92%
“…Briefly, 100e2000 ng of total RNA was reverse transcribed using the Omniscript Ò Reverse Transcription Kit (Qiagen Inc., Valencia, CA) following the manufacturer's protocol for first-strand cDNA synthesis from animal cells for 2-tube real-time RT-PCR. First-strand synthesis was accomplished using final concentrations of 1Â RT buffer, 2 U mL À1 RNase inhibitor, 0.5 mM of each dNTP, 0.2 U mL À1 Omniscript Ò reverse transcriptase, primed with 1 mM oligo dT 16 , and 10 mM random nonamers in a final reaction volume of 20 mL. Archival cDNA samples were prepared in 96-well PCR reaction plates (1 per tissue) and stored at À20 C until quantified by real-time PCR.…”
Section: Reverse Transcriptionmentioning
confidence: 99%