Use of mucus as a non‐invasive sampling method for DNA barcoding of stingrays and skates (batoid elasmobranchs)

Abstract

In this study we tested the use of mucus from five species of Neotropical marine batoid elasmobranchs to extract genomic DNA for barcoding and phylogenetic analysis. The DNA from all individuals sampled was successfully amplified and sequenced for molecular barcode, allowing 99–100% accuracy to the species level. This method proved to provide reliable and good‐quality DNA for barcoding and phylogenetic analysis of Neotropical elasmobranchs, through rapid handling and with low disturbance to animals.

Short Communication: Genetic variation of oceanic manta ray (Mobula birostris) based on mtDNA data in the Savu Sea, Indonesia

“…The results of genetic diversity from ND5 loci (Table 3) of oceanic manta rays in the Savu Sea has a value is 0.629 (h=0.629). The different result happened in other species of mobulids such as Benfin devil ray (M. thrustoni) (h=0.222) from COI locus (Domingues et al 2019). However, both result genetic diversity of oceanic manta ray on this research (ND5 loci) and Benfin devil ray (COI) from Domingues et al (2019) could not be actually compared because of different markers or loci (Hoffman et al 2009).…”

“…The different result happened in other species of mobulids such as Benfin devil ray (M. thrustoni) (h=0.222) from COI locus (Domingues et al 2019). However, both result genetic diversity of oceanic manta ray on this research (ND5 loci) and Benfin devil ray (COI) from Domingues et al (2019) could not be actually compared because of different markers or loci (Hoffman et al 2009). Otherwise, nucleotide diversity of oceanic manta rays in the Savu Sea value is deemed to be low (π=0.006), which stated that although the samples have a high genetic variation (haplotype), the variation within each individual sample is not very significant (probably less than ten base differences on each haplotype).…”

Molecular tools for assuring human health and environment-friendly frozen shellfish products in the United Arab Emirates markets

“…This approach provided key insights for the identification of correct species in different markets resolving many issues related to mislabeling, misnaming, and even illegal/unpleasant substitutions of species ( Do et al, 2019 , Xiong et al, 2016 , Xiong et al, 2019 , Abdelbaset-Donya et al, 2020 ). Moreover, molecular phylogenies appended to DNA barcoding contributed to accurate species identification ( Galal-Khallaf et al, 2017 , Galal-Khallaf et al, 2019 , Domingues et al, 2019 ). Sequencing of the shellfish 16S rDNA inter-specific hypervariable region was shown to be a good marker to differentiate shellfish species ( Kang et al, 2015 , Galal-Khallaf et al, 2016 ).…”