Use of Mueller matrix colposcopy in the characterization of cervical collagen anisotropy

Chue-Sang, Joseph; Holness, Nola; Gonzalez, Mariacarla; Greaves, Joan; Saytashev, Ilyas; Stoff, Susan; Gandjbakhche, Amir; Chernomordik, Viktor V.; Burkett, Gene; Ramella‐Roman, Jessica C.

doi:10.1117/1.jbo.23.12.121605

Cited by 48 publications

(34 citation statements)

References 45 publications

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“…The results showed accordance with current literature about the depolarization and retardance behavior of healthy cervices. 32,37,39,44 Due to the dependence spatial interference pattern of the current methodology, there are limitations on the deployment and data analysis, as well as the limitation of non-realtime feedback. Recent advancement in polarization technology may lead to new direction of this research.…”

Section: Resultsmentioning

confidence: 99%

“…Our group has used MMI to target the fibrous ultrastructure (i.e., collagen) organization of the cervix. 42 We have developed a clinical Mueller matrix system 43,44 based on a standard colposcope with high sensitivity to the cervix ultrastructure. We have tested the system on healthy volunteers, as well as have measured collagen arrangement and distribution non-invasively, and have positively compared our findings to other microscopic techniques, such as optical coherence tomography.…”

Section: Introductionmentioning

confidence: 99%

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Design and implementation of a portable colposcope Mueller matrix polarimeter

et al. 2020

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Section: Resultsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Design and implementation of a portable colposcope Mueller matrix polarimeter

et al. 2020

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“…Since many healthy collagen-rich tissues behave as uniaxial birefringent media, the azimuth of optical axis of linear retardation related to collagen alignment can often be measured. 21,22,27,51,59,77,78 Chicken or cow tendons have been used by many groups 45,50,60,[73][74][75][76] to validate polarization-based optical instruments. Azimuth angle is calculated 45,50,58,60,[72][73][74][75][76][79][80][81][82][83] as well as an increase in scalar retardance due to birefringence.…”

Section: Biological Phantomsmentioning

confidence: 99%

Optical phantoms for biomedical polarimetry: a review

et al. 2019

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Calibration, quantification, and standardization of the polarimetric instrumentation, as well as interpretation and understanding of the obtained data, require the development and use of well-calibrated phantoms and standards. We reviewed the status of tissue phantoms for a variety of applications in polarimetry; more than 500 papers are considered. We divided the phantoms into five groups according to their origin (biological/nonbiological) and fundamental polarimetric properties of retardation, depolarization, and diattenuation. We found that, while biological media are generally depolarizing, retarding, and diattenuating, only one of all the phantoms reviewed incorporated all these properties, and few considered at least combined retardation and depolarization. Samples derived from biological tissue, such as tendon and muscle, remain extremely popular to quickly ascertain a polarimetric system, but do not provide quantifiable results aside from relative direction of their principal optical axis. Microspheres suspensions are the most utilized phantoms for depolarization, and combined with theoretical models can offer true quantification of depolarization or degree of polarization. There is a real paucity of birefringent phantoms despite the retardance being one of the most interesting parameters measurable with polarization techniques. Therefore, future work should be directed at generating truly reliable and repeatable phantoms for this metric determination. Diattenuating phantoms are rare and application-specific. Given that diattenuation is considered to be low in most biological tissues, the lack of such phantoms is seen as less problematic. The heterogeneity of the phantoms reviewed points to a critical need for standardization in this field. Ultimately, all research groups involved in polarimetric studies and instruments development would benefit from sharing a limited set of standardized polarimetric phantoms, as is done earlier in the round robin investigations in ellipsometry.

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“…suggests using them as optical markers for diagnostics in clinical settings. [1][2][3][4][5] However, using these parameters for diagnostics requires (i) understanding the fundamental processes of interaction of polarized light with tissue and (ii) finding the optimal set of optical parameters, which will increase the accuracy of diagnostics.…”

Section: Introductionmentioning

confidence: 99%

“…These instruments may operate in a spectroscopic or angular-resolved mode 7,8 and may also provide both microscopic (few hundreds of μm 2 ) and macroscopic (few cm 2 ) polarimetric images of the sample. [2][3][4]5,9,10 MM contains all information on polarimetric and depolarizing properties of a sample. However, a straightforward physical interpretation of MM elements is possible for a quite limited set of samples like basic polarimetric elements (polarizers and wave plates) and partial depolarizers.…”

Section: Introductionmentioning

confidence: 99%

Digital histology with Mueller microscopy: how to mitigate an impact of tissue cut thickness fluctuations

Lee

Yoo

et al. 2019

J. Biomed. Opt.

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Mueller microscopy studies of fixed unstained histological cuts of human skin models were combined with an analysis of experimental data within the framework of differential Mueller matrix (MM) formalism. A custom-built Mueller polarimetric microscope was used in transmission configuration for the optical measurements of skin tissue model adjacent cuts of various nominal thicknesses (5 to 30 μm). The maps of both depolarization and polarization parameters were calculated from the corresponding microscopic MM images by applying a logarithmic Mueller matrix decomposition (LMMD) pixelwise. The parameters derived from LMMD of measured tissue cuts and the intensity of transmitted light were used for an automated segmentation of microscopy images to delineate dermal and epidermal layers. The quadratic dependence of depolarization parameters and linear dependence of polarization parameters on thickness, as predicted by the theory, was confirmed in our measurements. These findings pave the way toward digital histology with polarized light by presenting the combination of optimal optical markers, which allows mitigating the impact of tissue cut thickness fluctuations and increases the contrast of polarimetric images for tissue diagnostics.

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Use of Mueller matrix colposcopy in the characterization of cervical collagen anisotropy

Cited by 48 publications

References 45 publications

Design and implementation of a portable colposcope Mueller matrix polarimeter

Design and implementation of a portable colposcope Mueller matrix polarimeter

Optical phantoms for biomedical polarimetry: a review

Digital histology with Mueller microscopy: how to mitigate an impact of tissue cut thickness fluctuations

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