Use of Multicomponent Cryoprotective Media During Cryopreservation of Murine Embryos by Vitriﬁcation

Shakhova, Yuliia; Paliy, Anatoliy; Paliy, Andriy; Shigimaga, V. A.; Kis, Victor; Ivanov, V.

doi:10.15407/cryo30.02.203

Cited by 4 publications

(1 citation statement)

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“…Sucrose, glycerin, sorbitol, and skim milk have been shown to be the most effective protective agents for L. bulgaricus during lyophilization (Huang et al, 2006). Sucrose, as a cryoprotectant, is widely used in the preservation of various biological objects (Schoug et al, 2006;Shakhova et al, 2020). However, it has been reported that the addition of trehalose or sucrose does not increase the survival of lactic acid bacteria and yeast after freeze-drying (Bolla et al, 2011).…”

Section: Selection Of Technological Regime and Cryoprotectormentioning

confidence: 99%

Selection of technological regime and cryoprotector for lyophilization of lactobacteria (Lactobacillus spp.)

Paliy¹,

Gujvinska²,

Al-Rawashdeh³

2020

Ukr J Ecol

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Despite the success achieved in the comprehensive study of probiotic cultures, today there are a number of problems associated with the low viability of lactic acid bacteria during their processing and long-term storage in probiotics. Our work aimed to select the optimal technological regime and cryoprotectant to preserve the viability of lactic acid bacteria Lactobacillus spp. during their lyophilization. According to the results of the conducted researches, it is established that for freeze-drying of probiotic cultures Lactobacillus spp. in the facility LZ-45.27 (Frigera, Czech Republic) the most optimal is the mode which provides a rise of temperature within 45 hours from minus 70.0±1.0 °C to plus 26.0±1.0 °C with a speed of 2.2±0.1 °C/hour. It is effective to use protective media for lactobacilli, which consist of: skim milk (90%) and sucrose (10%); skim milk (90%) and lactose (10%); skim milk (90%), glucose (2.5%), sucrose (2.5%), lactose (5.0%) (P≤0.05). Freeze-drying of lactic acid bacteria under optimal conditions and the addition of cryoprotectants will avoid the problems associated with a significant reduction in the number of microbial cells. The results of research can be used for long-term storage of cultures of lactobacilli by their lyophilization.

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Section: Selection Of Technological Regime and Cryoprotectormentioning

confidence: 99%

Selection of technological regime and cryoprotector for lyophilization of lactobacteria (Lactobacillus spp.)

Paliy¹,

Gujvinska²,

Al-Rawashdeh³

2020

Ukr J Ecol

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Mathematical model implementation in conditions of uncertainty and possible risks

Kravtsov¹,

Levkin²,

Makarov³

2021

Energy and automation

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The article presents the theoretical and methodological principles for forecasting and mathematical modeling of possible risks in technological and biotechnological systems. The authors investigated in details the possible approach to the calculation of the goal function and its parameters. Considerable attention is paid to substantiating the correctness of boundary value problems and Cauchy problems. In mechanics, engineering, and biology, Cauchy problems and boundary value problems of differential equations are used to model physical processes. It is important that differential equations have a single physically sound solution. The authors of this article investigate the specific features of boundary value problems and Cauchy problems with boundary conditions in a two-point medium, and determine the conditions for the correctness of such problems in the spaces of power growth functions. The theory of pseudo-differential operators in the space of generalized functions was used to prove the correctness of boundary value problems. The application of the obtained results will make it possible to guarantee the correctness of mathematical models built in conditions of uncertainty and possible risks. As an example of a computational mathematical model that describes the state of the studied object of non-standard shape, the authors considered the boundary value problem of the system of differential equations of thermal conductivity for the embryo under the action of a laser beam. For such a boundary value problem, it is impossible to guarantee the existence and uniqueness of the solution of the system of differential equations. To be sure of the existence of a single solution, it is necessary either not to take into account the three-layer structure of the microbiological object, or to determine the conditions for the correctness of the boundary value problem. Applying the results obtained by the authors, the correctness of the boundary value problem of systems of differential equations of thermal conductivity for the embryo is proved taking into account the three-layer structure of the microbiological object. This makes it possible to increase the accuracy and speed of its implementation on the computer. Key words: forecasting, risk, correctness, boundary value problems, conditions of uncertainty

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Correction of the sexual function in domestic animals by megestrol acetate

Paliy

Dotsenko

Pavlichenko³

et al. 2022

J. Vet. Med. Biotechnol. Biosafety

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Correction of sexual function in animals is a topical issue today, despite a large amount of scientific data on this problem. The study aimed to scientifically substantiate the effectiveness of hormonal veterinary drugs in cats and dogs of different breeds and genders. Veterinary drugs with the active substance megestrol acetate were used in the experiments. The studies were performed on clinically healthy cats and dogs of both sexes, different breeds, and ages vaccinated against infectious diseases and treated with antiparasitic drugs. The study was conducted according to the current regulatory documentation. It has been found that the use of hormonal drugs to interrupt/delay estrus in female cats at a dose of 5 mg of megestrol acetate for 8 days leads to a significant decrease in follicle-stimulating hormone, and the content of luteinizing hormone in the blood of cats in the experimental groups reliably decreased throughout the experiment. The use of contraceptives based on megestrol acetate in doses of 2.5 mg for 14 days for cats leads to a reliable decrease in the concentration of testosterone in the serum. It was found that the use of experimental hormonal drugs for female dogs in doses of 5 mg of megestrol acetate per 2.5 kg of body weight in the first 3 days, and half the daily dose from the 4th to the 10th day, leads to a reliable decrease in concentrations of follicle-stimulating and luteinizing hormones in serum compared to the control. Oral administration of experimental hormonal drugs to male dogs in doses of 5 mg of megestrol acetate per 2.5 kg of body weight for 8 days, led to a reliable decrease in the concentration of testosterone in the serum of males. It was found that the estrous cycle in female cats and dogs of the experimental groups after the cessation of hormonal drugs was completely restored, which indicates the safety of drugs with an active ingredient megestrol acetate

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Use of Multicomponent Cryoprotective Media During Cryopreservation of Murine Embryos by Vitriﬁcation

Cited by 4 publications

References 1 publication

Selection of technological regime and cryoprotector for lyophilization of lactobacteria (Lactobacillus spp.)

Selection of technological regime and cryoprotector for lyophilization of lactobacteria (Lactobacillus spp.)

Mathematical model implementation in conditions of uncertainty and possible risks

Correction of the sexual function in domestic animals by megestrol acetate

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