Use of Natural Minerals to Immobilize Bacterial Cells for Remediating Cracks in Cement-Based Materials

Tezer, Mustafa Mert; Bundur, Zeynep Basaran

doi:10.1061/(asce)mt.1943-5533.0004098

Cited by 14 publications

(7 citation statements)

References 31 publications

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“…5). Although the soil bacteria sample had less visual crack healing than the B. subtilis sample, its UPV remained higher than that of its non-bacterial counterpart, suggesting that crack closure may have occurred through the depth of the crack rather than only at the mouth of the crack by penetration of nutrients through the crack depth [26]. CSA samples yielded lower velocities compared to OPC samples.…”

Section: Fig 2 Relative Abundance Of Microorganisms At Genus Level (A...mentioning

confidence: 86%

Novelty in bacteria source production and concrete binders in self-healing cementitious samples

Acartürk¹,

Straathof²,

Liu³

et al. 2023

MATEC Web Conf.

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One of the challenges associated with creating bacterial-concrete systems capable of biomineralizing CaCO3 to fill cracks is the high pH environment of the hydrated cement paste. In this study two approaches to address this challenge were investigated: (i) the use of calcium sulfoaluminate (CSA) cement, which develops a naturally lower pH, and (ii) the use of non-axenic bacterial cultures, which may facilitate growth of bacterial strains more resilient to harsh alkaline conditions. Axenic B. subtilis and a non-axenic bacterial system from soil were produced and utilized in ordinary portland cement (OPC) and CSA samples. The mechanical properties, water absorption, calcium carbonate precipitation capability, and survivability of bacteria were investigated. The highest B. subtilis and soil bacteria viability was obtained through use of CSA cement and may enable greater later age crack healing potential than mixtures using OPC. Incorporation of axenic bacteria resulted in increased bacteria survivability in the mortar samples when compared to non-axenic bacteria mixes. However, in both cementitious systems, use of B. subtilis and soil bacteria led to similar improvements, suggesting that non-axenic cultures may be used in concrete effectively.

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Section: Fig 2 Relative Abundance Of Microorganisms At Genus Level (A...mentioning

confidence: 86%

Novelty in bacteria source production and concrete binders in self-healing cementitious samples

Acartürk¹,

Straathof²,

Liu³

et al. 2023

MATEC Web Conf.

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“…Utilizing natural minerals as concrete components from a civil engineering standpoint demonstrates that self-healing in cement-based systems can be achieved by inducing biogenic calcium carbonate (CaCO) precipitation (Mert Tezer & Başaran Bundur, 2022). Natural materials are predominantly employed in the construction industry (in addition to concrete mixtures, the production of pavers, and gravel) or in water purification (Strejcová et al, 2020).…”

Section: Figure 1 Map Of Pamekasan Madura Regencymentioning

confidence: 99%

XRF and XRD Testing for Sand Mineral Content Identification at Talang Siring Beach

Ariyanto

Joni

Yunanto

2023

Edu. Jur. Pend. Fis

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In the Talang siring tour of the Pamekasan district, numerous illegal mining operations along the coast for sale and personal use produce building materials with low economic value. However, if the sand is processed further, it will produce materials with high technology utilization and economic value. This research is a preliminary investigation into converting sand into high-value minerals to be utilized economically. Using X-Ray Fluorescence (XRF) and X-Ray Diffraction (XRD) experiments, this study aims to determine the sand's mineral content in the Talang Siring tourist area in Pamekasan Regency. Initial identification is the XRF test function for analyzing sand's chemical elements and composition. The purpose of XRD is to analyze the crystal structure and correlate the results with Macth and origin software based on the XRF results. The results of the XRF test indicated that Silicone had the highest concentration of minerals at 63.8%, followed by Calcium at 29.4% and Iron at 2.45%. The XRD test results indicated that the sand's mineral composition was qualitatively SiO2 90%, CaCO3 8%, Iron 1%, and the remainder 1%.

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“…The nutrient medium was sterilized at 121˚C for 45 minutes (NUVE NC 40M, Istanbul, Turkey). The cells were grown in the sterilized nutrient medium with shaking conditions (175 rpm) at 30˚C until the stationary phase was obtained (10 9 Colony Forming Unit (CFU)/mL) [12]. After the incubation, the cells were collected from the culture by centrifuging at 8,000 g for 10 minutes.…”

Section: Microorganism Selection and Growthmentioning

confidence: 99%

“…Previously, our studies showed that natural minerals such as sepiolite, diatomaceous earth, bentonite, and pumice could be used to immobilize bacterial cells to trigger self-healing in mortars [12,13]. Herein, the biological self-healing additive effectively remediates flexural cracks in less than 21 days.…”

Section: Introductionmentioning

confidence: 98%

The performance evaluation of protection barriers in bacterial self-healing mortar

2023

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The early age microcracking is a significant problem in concrete structures resulting in increased permeability and decreased durability. The previous work showed that Sporasarcina pasteurii cells immobilized on natural minerals such as bentonite, diatomaceous earth, sepiolite, and pumice effectively remediated early-age microcracks in the cementitious systems by triggering microbial-induced calcite precipitation (MICP). This promising approach can solve early-age shrinkage cracking in cementitious systems. Therefore, it is essential to assess the impact of self-healing additives on drying shrinkage. This study investigates the influence of mineral-based biological additives on the drying shrinkage capacity of cement-based mortar and the possible self-healing of cracks if any occur. To achieve this goal, the free shrinkage in control (containing only minerals) and bacterial (containing bio-based additive) samples were measured based on ASTM 596-18 norms. Moreover, the performance assessment of developed self-healing additives was done by determining compressive strength and initial setting time of bacterial self-healing mortar.

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Use of Natural Minerals to Immobilize Bacterial Cells for Remediating Cracks in Cement-Based Materials

Cited by 14 publications

References 31 publications

Novelty in bacteria source production and concrete binders in self-healing cementitious samples

Novelty in bacteria source production and concrete binders in self-healing cementitious samples

XRF and XRD Testing for Sand Mineral Content Identification at Talang Siring Beach

The performance evaluation of protection barriers in bacterial self-healing mortar

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