Use of Non-Human Plasma for in Vitro Cultivation and Antimalarial Drug Susceptibility Testing of Plasmodium Falciparum

Oduola, A. M. J.; Alexander, Bruce M.; Weatherly, Norman F.; Bowdre, Jean H.; Desjardins, Robert E.

doi:10.4269/ajtmh.1985.34.209

Cited by 13 publications

(16 citation statements)

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“…Because of these disadvantages, numerous alternative sources of sera and nonserum substitutes have been tested in the past (1,6,13,15,19,27,28,37). Although some of these substitutes have been successfully used for the continuous cultivation of laboratory-adapted P. falciparum strains and clones, they are generally less effective than human serum and have not been adopted by many research laboratories.…”

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confidence: 99%

In Vitro Culture and Drug Sensitivity Assay of Plasmodium falciparum with Nonserum Substitute and Acute-Phase Sera

et al. 1999

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The short-term in vitro growth of Plasmodium falciparumparasites in the asexual erythrocytic stage and the in vitro activities of eight standard antimalarial drugs were assessed and compared by using RPMI 1640 medium supplemented with 10% nonimmune human serum, 10% autologous or homologous acute-phase serum, or 0.5% Albumax I (lipid-enriched bovine serum albumin). In general, parasite growth was maximal with autologous (or homologous) serum, followed by Albumax I and nonimmune serum. The 50% inhibitory concentrations (IC50s) varied widely, depending on the serum or serum substitute. The comparison of IC50s between assays with autologous and nonimmune sera showed that monodesethylamodiaquine, halofantrine, pyrimethamine, and cycloguanil had similar IC50s. Although the IC50s of chloroquine, monodesethylamodiaquine, and dihydroartemisinin were similar with Albumax I and autologous sera, the IC50s of all test compounds obtained with Albumax I differed considerably from the corresponding values obtained with nonimmune serum. Our results suggest that Albumax I and autologous and homologous sera from symptomatic, malaria-infected patients may be useful alternative sources of serum for in vitro culture of P. falciparum isolates in the field. However, autologous sera and Albumax I do not seem to be suitable for the standardization of isotopic in vitro assays for all antimalarial drugs.

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In Vitro Culture and Drug Sensitivity Assay of Plasmodium falciparum with Nonserum Substitute and Acute-Phase Sera

et al. 1999

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“…The lower growth rate with goat plasma, adaptation to goat serum during the long-term cultivation, and batch-to-batch difference of goat sera may explain in part the discordance of results between the studies of Oduola and others and the present study. Although some investigators have reported a successful cultivation of laboratory-adapted P. falciparum strains in RPMI 1640 supplemented with 10% rabbit or horse serum, 21,22 parasite growth was poor in these media in this study. The likely explanation is the batch-to-batch variation of the nutritional quality of animal sera.…”

Section: Discussionmentioning

confidence: 63%

“…A long-term cultivation has also been successfully performed with goat serum. 21 In their study, Oduola and others showed that chloroquine IC 50 values did not differ significantly between RPMI 1640 medium supplemented with human plasma or goat plasma. 21 Unlike in this study, once adapted to goat serum, laboratory strains of P. falciparum generally grew slightly less in the medium supplemented with goat plasma.…”

Section: Discussionmentioning

confidence: 90%

“…21 In their study, Oduola and others showed that chloroquine IC 50 values did not differ significantly between RPMI 1640 medium supplemented with human plasma or goat plasma. 21 Unlike in this study, once adapted to goat serum, laboratory strains of P. falciparum generally grew slightly less in the medium supplemented with goat plasma. The lower growth rate with goat plasma, adaptation to goat serum during the long-term cultivation, and batch-to-batch difference of goat sera may explain in part the discordance of results between the studies of Oduola and others and the present study.…”

Section: Discussionmentioning

confidence: 90%

“…Sera from some other animals (e.g., pigs and sheep) do not support P. falciparum growth in vitro and were not tested in this study. 21,23,24 In summary, the capacity of several commercially available media, animal sera, and serum-free media to support the in vitro growth of fresh isolates during the first erythrocytic cycle was assessed with the aim to optimize drug assays, both in this study and in previous studies. 17 The results suggest that DME plus 10% human serum, IMDM plus 10% human serum, RPMI 1640 medium plus 10% goat serum, RPMI 1640 medium plus 10% fetal calf serum, RPMI 1640 medium plus 0.5% Albumax, and serum-free GIT are of potential interest.…”

Section: Discussionmentioning

confidence: 99%

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Molecular Epidemiology of Malaria in Cameroon. Xxiii. Experimental Studies on Serum Substitutes and Alternative Culture Media for in Vitro Drug Sensitivity Assays Using Clinical Isolates of Plasmodium Falciparum

Basco¹

2006

The American Journal of Tropical Medicine and Hygiene

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Correlation studies on the in vitro drug response of field isolates of Plasmodium falciparum and molecular markers for drug resistance are becoming important as many malaria control programs abandon monotherapies and resort to combination therapies. The standardization and optimization of the in vitro drug sensitivity assay are one of the prerequisites for validating molecular markers in the field. The present study was designed to assess and compare the growth of freshly obtained isolates for at least the first erythrocytic cycle in various culture media and determine the in vitro response to chloroquine in alternative media. Parasite growth was consistently higher in Dulbecco's modified Eagle's medium (DME)-human serum, Iscove's modified Dulbecco's medium (IMDM)-human serum, RPMI 1640 medium-goat serum, and a serum-free medium containing 1:1 (v/v) mixture of IMDM and F-12 supplemented with an ammonium sulfate fraction of adult bovine serum than in RPMI 1640 medium-human serum mixture. The level of chloroquine response determined in human serum-supplemented DME, IMDM, and RPMI 1640 media did not differ significantly (P > 0.05) from the control (RPMI 1640-human serum). This study suggests that alternative media may be used to optimize parasite growth during the critical initial phase of transition from in vivo to in vitro conditions. The capacity of these media to support long-term cultivation of P. falciparum requires further investigation.

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Plasmodium falciparum: Cloning by single-erythrocyte micromanipulation and heterogeneity in vitro

Oduola

Weatherly

Bowdre

et al. 1988

Experimental Parasitology

129

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Use of Non-Human Plasma for in Vitro Cultivation and Antimalarial Drug Susceptibility Testing of Plasmodium Falciparum

Cited by 13 publications

References 0 publications

In Vitro Culture and Drug Sensitivity Assay of Plasmodium falciparum with Nonserum Substitute and Acute-Phase Sera

In Vitro Culture and Drug Sensitivity Assay of Plasmodium falciparum with Nonserum Substitute and Acute-Phase Sera

Molecular Epidemiology of Malaria in Cameroon. Xxiii. Experimental Studies on Serum Substitutes and Alternative Culture Media for in Vitro Drug Sensitivity Assays Using Clinical Isolates of Plasmodium Falciparum

Plasmodium falciparum: Cloning by single-erythrocyte micromanipulation and heterogeneity in vitro

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