Use of peltier thermoelectric devices to control column temperature in high-performance capillary electrophoresis

Nelson, Robert; Paulus, Aran; Cohen, Aharon S.; Guttman, András; Karger, Barry L.

doi:10.1016/s0021-9673(01)84282-5

Cited by 167 publications

(73 citation statements)

References 21 publications

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“…employed a constant value as the temperature difference for the analysis of LYS using an air-cooling instrument.20 So far, several groups had reported on the measurement or estimation of the actual temperature by the use of the thermal properties of the capillary and the inside solution26,27, a micro thermocouple28, the absorbance of a Co(II) solution29, thermodynamic parameters of the solute distribution between micelle and water in MEKC30, the conductivities at low and high voltages31, and the difference in electroosmotic flow at low and high voltages.31 However, some methods have limitations to be applied to this case, e.g., the thermal properties at every temperature employed would be needed for the method described in ref. 26, the reproducible set-up of the system would be difficult for the thermocouple method, and free solutions without micelles and coated capillaries could not be used for the MEKC method and the electroosmosis method, respectively. Therefore, the Co(II) and the conductivity methods were applied.…”

Section: Resultsmentioning

confidence: 99%

Nano-Scale Monitoring of the Thermally-Induced Unfolding of Proteins Using Capillary Electrophoresis with In-Column Incubation

et al. 1997

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Based on the difference between the electrophoretic mobility of the native protein and that of the unfolded one, the thermally-induced unfolding transition of proteins was analyzed by capillary electrophoresis (CE) using an in-column incubation method. In this method, the required amount of sample could be reduced to 100 ng because the unfolding process occurred not in the sample vial, but in the separation capillary after the sample injection. The thermodynamic parameters of the unfolding transition process were obtained by analyzing the dependence of the electrophoretic mobilities on the temperature inside the capillary controlled by the thermostated water. The temperature inside the capillary was measured by an easy and rapid method on the basis of the dependence of the electrical current on the applied voltage. The electrophoretic mobility was converted to a temperature-independent parameter using the average of the electrical current through the analysis. In this work, the unfolding processes of several proteins were investigated. Although the apparent transition temperatures by CE were generally consistent with those obtained by far-UV CD using a two-state transition model, these two methods had different detection selectivity for the change in the protein structures. The CE method would offer additional information for the unfolding processes of proteins.

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Section: Resultsmentioning

confidence: 99%

Nano-Scale Monitoring of the Thermally-Induced Unfolding of Proteins Using Capillary Electrophoresis with In-Column Incubation

et al. 1997

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“…used 30 mM phosphate buffer of pH 2.4 as the BGE for the chiral resolution of biphenyl pesticides. Nelson et al [34] reported no heating of the capillary up to 30 kV as the applied voltage when borate buffer was used while heating of the capillary was observed even at 10 and 12 kV using CAPS and phosphate buffers respectively.…”

Section: Optimizationmentioning

confidence: 99%

Chiral Analyses of Pollutants by Capillary Electrophoresis

Ali¹

2010

TOCBMJ

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Abstract:The determination of enantiomeric composition of chiral xenobiotics and pollutants is a very difficult job due to their low amounts and poor detection by commonly used UV detector. But this sort of analysis is an important issue from the health point of view. The chiral analyses of these notorious pollutants by capillary electrophoresis have been discussed in this article. This review discusses the new trends and advancements, which have been achieved in the analyses of chiral pollutants using capillary electrophoresis and the state-of-art of their enaniomeric resolution. This article focuses on sample treatment, applications, optimization, detection, mechanisms of chiral resolution and future perspectives of CE in chiral resolution of xenobiotics. Besides, efforts have also been made to suggest the improvement in CE machine to make it ideal for the analyses of chiral xenobiotics at trace levels.

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“…The threshold for Joule heating occurs when the voltage-current behavior of the pump ceases to be linear. At this point, the system begins to heat up, and the increase in temperature changes the physical behavior of the pumped fluid [19]. For each of these thresholds, we also calculated the corresponding power dissipation Q diss [15]:…”

Section: Joule Heating and Power Consumptionmentioning

confidence: 99%

Magnetohydrodynamic pumping in nuclear magnetic resonance environments

Homsy

Linder

Lucklum

et al. 2007

Sensors and Actuators B: Chemical

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We present a DC magnetohydrodynamic (MHD) pump as component of a nuclear magnetic resonance (NMR) microfluidic chip. This is the first time that MHD pumping in an NMR environment was observed and demonstrated. This chip generates a maximum flow rate of 1.5 L min −1 (2.8 mm s −1 in the microchannel) for an applied voltage of 19 V with only 38 mW of power consumption in a 7 T superconductive magnet. We developed a simple method of flow rate measurement inside the bulky NMR magnet by monitoring the displacement of a liquid-liquid interface of two immiscible liquids in an off-chip capillary. We compared and validated this flow measurement technique with another established technique for microfluidics based on the displacement of microbeads. This allowed us to characterize and compare the flow rate generated by the micropump on top of a permanent magnet (B 1 = 0.33 T) with the superconductive magnet (B 0 = 7.05 T). We observed a 21-fold increase in flow rate corresponding to the ratio of the magnetic field intensities (B 0 /B 1 = 21) in accordance with the theoretical flow dependence on the magnetic field intensity. The final aim is to integrate MHD pumps together with planar coils in a microfluidic system for NMR analysis. The high performance of MHD pumps at relatively low flow rates is seen as an asset for NMR and MRI applications.

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Use of peltier thermoelectric devices to control column temperature in high-performance capillary electrophoresis

Cited by 167 publications

References 21 publications

Nano-Scale Monitoring of the Thermally-Induced Unfolding of Proteins Using Capillary Electrophoresis with In-Column Incubation

Nano-Scale Monitoring of the Thermally-Induced Unfolding of Proteins Using Capillary Electrophoresis with In-Column Incubation

Chiral Analyses of Pollutants by Capillary Electrophoresis

Magnetohydrodynamic pumping in nuclear magnetic resonance environments

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