Use of photodynamic therapy and photobiomodulation as alternatives for microbial control on clinical and subclinical mastitis in sheep

Silva, Lara Oliveira; Souza, Kedma Lorena Da Silva; Beloti, Larissa de Jesus; Neto, Waldemar Mota Ramos; Núñez, Silvia Cristina; Frias, Danila Fernanda Rodrigues

doi:10.1007/s10103-022-03506-2

Cited by 5 publications

(2 citation statements)

References 31 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…However, limited research has explored its application in bovine mastitis pathogens and drug-resistant bacteria. Silva et al [ 17 ] evaluated the effect of photoinactivation on strains of clinical and subclinical mastitis isolated from sheep, and concluded that this treatment is promissory for bovine mastitis. Furthermore, Sellera et al [ 6 ] demonstrated the efficacy of PDI on different antibiotic-resistant species isolated from bovine mastitis.…”

Section: Discussionmentioning

confidence: 99%

The Impact of Low-Level Benzalkonium Chloride Exposure on Staphylococcus spp. Strains and Control by Photoinactivation

Bonsaglia,

Calvo,

Sordelli

et al. 2023

Antibiotics

View full text Add to dashboard Cite

Exposure of bacteria to low concentrations of biocides can facilitate horizontal gene transfer, which may lead to bacterial adaptive responses and resistance to antimicrobial agents. The emergence of antibacterial resistance not only poses a significant concern to the dairy industry but also adds to the complexity and cost of mastitis treatment. This study was aimed to evaluate how selective stress induced by benzalkonium chloride (BC) promotes antibiotic non-susceptibility in Staphylococcus spp. In addition, we investigated the efficacy of photodynamic inactivation (PDI) in both resistant and susceptible strains. The study determined the minimum inhibitory concentration (MIC) of BC using the broth microdilution method for different Staphylococcus strains. The experiments involved pairing strains carrying the qacA/qacC resistance genes with susceptible strains and exposing them to subinhibitory concentrations of BC for 72 h. The recovered isolates were tested for MIC BC and subjected to disc diffusion tests to assess changes in susceptibility patterns. The results demonstrated that subinhibitory concentrations of BC could select strains with reduced susceptibility and antibiotic resistance, particularly in the presence of S. pasteuri. The results of PDI mediated by toluidine blue (100 µM) followed by 60 min irradiation (total light dose of 2.5 J/cm2) were highly effective, showing complete inactivation for some bacterial strains and a reduction of up to 5 logs in others.

show abstract

Section: Discussionmentioning

confidence: 99%

The Impact of Low-Level Benzalkonium Chloride Exposure on Staphylococcus spp. Strains and Control by Photoinactivation

Bonsaglia,

Calvo,

Sordelli

et al. 2023

Antibiotics

View full text Add to dashboard Cite

show abstract

“…Furthermore, in sheep with mastitis, PDT reduced CNS, Streptococcus spp. And E. coli within udders ( 151 ). Though PDT has much promise to treat mastitis, the method is still in initial research stages.…”

Section: Other Therapiesmentioning

confidence: 99%

Alternatives to antibiotics for treatment of mastitis in dairy cows

Liang

et al. 2023

Front. Vet. Sci.

View full text Add to dashboard Cite

Mastitis is considered the costliest disease on dairy farms and also adversely affects animal welfare. As treatment (and to a lesser extent prevention) of mastitis rely heavily on antibiotics, there are increasing concerns in veterinary and human medicine regarding development of antimicrobial resistance. Furthermore, with genes conferring resistance being capable of transfer to heterologous strains, reducing resistance in strains of animal origin should have positive impacts on humans. This article briefly reviews potential roles of non-steroidal anti-inflammatory drugs (NSAIDs), herbal medicines, antimicrobial peptides (AMPs), bacteriophages and their lytic enzymes, vaccination and other emerging therapies for prevention and treatment of mastitis in dairy cows. Although many of these approaches currently lack proven therapeutic efficacy, at least some may gradually replace antibiotics, especially as drug-resistant bacteria are proliferating globally.

show abstract