Use of Physcion to Improve Atopic Dermatitis-Like Skin Lesions through Blocking of Thymic Stromal Lymphopoietin

Abstract

Physcion is well known for the treatment of carcinoma. However, the therapeutic effect of physcion on atopic dermatitis (AD) through the inhibition of thymic stromal lymphopoietin (TSLP) level remains largely unknown. In this study, we investigated the anti-AD effect of physcion using HMC-1 cells, splenocytes, and a murine model. Treatment with physcion decreased production and mRNA expression levels of TSLP, IL-6, TNF-ɑ, and IL-1β in activated HMC-1 cells. Physcion reduced the expression levels of RIP2/caspase-1 and phospho (p)ERK/pJNK/pp38 in activated HMC-1 cells. Physcion suppressed the expression levels of pIKKβ/NF-κB/pIkB in activated HMC-1 cells. Moreover, physcion attenuated the production levels of TSLP, IL-4, IL-6, TNF-, and IFN-γ from activated splenocytes. Oral administration of physcion improved the severity of 2,4-dinitrochlorobenzene-induced AD-like lesional skin through reducing infiltration of inflammatory cells and mast cells, and the protein and mRNA levels of TSLP, IL-4, and IL-6 in the lesional skin tissues. Physcion attenuated histamine, IgE, TSLP, IL-4, IL-6, and TNF- levels in serum. In addition, physcion inhibited caspase-1 activation in the lesional skin tissues. These findings indicate that physcion could ameliorate AD-like skin lesions by inhibiting TSLP levels via caspase-1/MAPKs/NF-kB signalings, which would provide experimental evidence of the therapeutic potential of physcion for AD.

Chrysophanol, Physcion, Hesperidin and Curcumin Modulate the Gene Expression of Pro-Inflammatory Mediators Induced by LPS in HepG2: In Silico and Molecular Studies

“…The two structurally related anthraquinones (chrysophanol and physcion) showed similar behavior in vitro and in silico. On contrary to curcumin, both compounds showed their ability to bind effectively to all of the MAPKs enzymes but with lower extent in case of ERK1/2, which can be explained in the light of the previously mentioned criteria, and is in agreement with former reports indicating the ability of physcion glycoside to exhibit anti-inflammatory effect against rheumatoid arthritis derived fibroblast-like synoviocyte cell line by inhibiting phosphorylation of p38, JNK and ERK MAP kinase [48,49,50]. Yet, there were no studies addressing the ability of anthraquinones to inhibit MK-3.…”

Physcion Protects Against Ethanol-Induced Liver Injury by Reprogramming of Circadian Clock

“…The mice (250 or 500 μg kg −1 body weight) were randomly divided into a normal group, a model group, a low-concentration Physcion treatment group (250 μg kg −1 body weight), and a high-concentration Physcion treatment group (500 μg kg −1 body weight) ( Figure 1B ), with eight mice in each group ( Figure 1B ) ( Portari et al, 2016 ; Yao et al, 2017 ). Doses of Physcion were determined according to previous publications ( Shen et al, 2011 ; Moon et al, 2019 ). Physcion administration groups were pretreated with single dose of physcion by oral gavage for 14 consecutive days.…”

“…After trypsinization and passaging, cells were added into 6-well plates (5 × 10 5 cells/well). The treatment group comprised cells of fourth to seventh passages which were treated with physcion (0.125, 0.25 μm) and EtOH (50 mm) for 24 h ( Moon et al, 2019 ). For circadian synchronization by serum, cells were treated with medium containing 50% horse serum for 2 h and treated with EtOH (50 mM) or PHY (0.25 μM) for 24 h. The cells were prepared for qPCR to measure mRNA level at 4 h intervals from 24 h (ZT = 0) to 44 h (ZT = 24) time-points.…”

Role of Epithelium-Derived Cytokines in Atopic Dermatitis and Psoriasis: Evidence and Therapeutic Perspectives

“…Samples derived from murine skin, after its administration, demonstrated lower levels of many interleukins involved in the pathogenesis of AD, among which TSLP was arguably the most important. Further studies will be required, but positive results in this sense could validate a new possible treatment for this form of dermatosis [ 54 ]. Kim et al focused on an eastern Asia plant to alleviate AD manifestations and symptoms by blocking the activity of TSLP, leading to contrasting results.…”