Use of Porous Polystyrene Scaffolds to Bioengineer Human Epithelial Tissues In Vitro

Costello, Lydia; Darling, Nicole; Freer, Matthew; Bradbury, Steven P.; Mobbs, Claire

doi:10.1007/978-1-0716-1246-0_20

Cited by 11 publications

(8 citation statements)

References 14 publications

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“…1 D). These data are in agreement with previous studies demonstrating a higher ability of the dual compartment model to encourage cell terminal differentiation ( Costello et al, 2021 ; Darling et al, 2020 ).…”

Section: Resultssupporting

confidence: 93%

Cruciferous vegetable-derived indole-3-carbinol prevents coronavirus cell egression mechanisms in tracheal and intestinal 3D in vitro models.

et al. 2023

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Section: Resultssupporting

confidence: 93%

Cruciferous vegetable-derived indole-3-carbinol prevents coronavirus cell egression mechanisms in tracheal and intestinal 3D in vitro models.

et al. 2023

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“…63 3D engineered tissues provide a more physiological platform for investigations and bridge the gap between 2D cell culture and native tissues. 64,65 We demonstrate significant epidermal thickening of this tissue construct following EC23 treatment to a greater degree than ATRA. Similarly, in vivo application to mouse back skin also induced epidermal thickening of almost threefold compared to controls, whereas to achieve the same level of thickening ATRA treatment had to be either repeated 4 times or concentration increased 10-fold.…”

Section: Discussionmentioning

confidence: 75%

“…We tested the effect of EC23 on an epidermal equivalent which has previously shown responsiveness to commonly used retinoids 63 . 3D engineered tissues provide a more physiological platform for investigations and bridge the gap between 2D cell culture and native tissues 64,65 . We demonstrate significant epidermal thickening of this tissue construct following EC23 treatment to a greater degree than ATRA.…”

Section: Discussionmentioning

confidence: 94%

Regulation of epidermal proliferation and hair follicle cycling by synthetic photostable retinoid EC23

Määttå

Nixon

Robinson

et al. 2023

J of Cosmetic Dermatology

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Background Retinoid signaling is an important regulator of the epidermis and skin appendages. Therefore, synthetic retinoids have been developed for therapeutic use for skin disorders such as psoriasis and acne. Aims In previous studies, we showed how the photostable retinoid EC23 induces neuronal differentiation in stem cell‐like cell populations, and here, we aim to investigate its ability to influence epidermal and hair follicle growth. Methods EC23 influence on skin biology was investigated initially in cultures of monolayer keratinocytes and three‐dimentional in vitro models of skin, and finally in in vivo studies of mice back skin. Results EC23 induces keratinocyte hyperproliferation in vitro and in vivo, and when applied to mouse skin increases the number of involucrin‐positive suprabasal cell layers. These phenotypic changes are similar in skin treated with the natural retinoid all‐trans retinoic acid (ATRA); however, EC23 is more potent; a tenfold lower dose of EC23 is sufficient to induce epidermal thickening, and resulting hyperproliferation is sustained for a longer time period after first dose. EC23 treatment resulted in a disorganized stratum corneum, reduced cell surface lipids and compromised barrier, similar to ATRA treatment. However, EC23 induces a rapid telogen to anagen transition and hair re‐growth in 6‐week‐old mice with synchronously resting back skin follicles. The impact of EC23 on the hair cycle was surprising as similar results have not been seen with ATRA. Conclusions These data suggest that synthetic retinoid EC23 is a useful tool in exploring the turnover and differentiation of cells and has a potent effect on skin physiology.

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“…We first used commercially available Alvetex ® scaffolds made of porous cross-linked polystyrene (47). These are engineered to a thickness of 200 μm with pores around 36-40 μm, with a ‘sponge-like’ appearance (48). Like in 2D cultures, we coated the scaffold with full-length FN.…”

Section: Resultsmentioning

confidence: 99%

Inflammatory Synovial Fibroblast Culture in 3D Systems: A Comparative Transcriptomic and Functional Study

Khan

Dobre

Wang

et al. 2022

Preprint

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Inflammation is essential for responding to infections and subsequent tissue healing. However, chronic unresolved inflammation can become a serious health problem, as exemplified in the joints during Rheumatoid Arthritis (RA). Why does inflammation persist in RA? The answer could lie with synovial fibroblasts, non-haemopoietic cells that can adopt a pathogenic phenotype that fuels disease progression for years. Critically, targeting local fibroblasts could stop joint inflammation without suppressing systemic immunity. Nevertheless, basic research findings have not been translated to new drugs, perhaps because non-physiological data can be inadvertently generated in 2D cultures. Thus, developing better in vitro platforms is an urgent need in biomedical research. In this work, we sought to understand how distinct 3D environments affect fibroblast-mediated inflammation. Arthritic synovial fibroblasts were expanded and cultured in 2D, 3D rigid scaffolds and engineered hydrogels. The results reveal that SFs are plastic and adopt inflammatory or remission-like phenotypes in response to their surroundings. This work identifies new directions to develop better models for drug testing, and even signposts candidate mechanisms by which to rewire destructive SFs.

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Use of Porous Polystyrene Scaffolds to Bioengineer Human Epithelial Tissues In Vitro

Cited by 11 publications

References 14 publications

Cruciferous vegetable-derived indole-3-carbinol prevents coronavirus cell egression mechanisms in tracheal and intestinal 3D in vitro models.

Cruciferous vegetable-derived indole-3-carbinol prevents coronavirus cell egression mechanisms in tracheal and intestinal 3D in vitro models.

Regulation of epidermal proliferation and hair follicle cycling by synthetic photostable retinoid EC23

Inflammatory Synovial Fibroblast Culture in 3D Systems: A Comparative Transcriptomic and Functional Study

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