Use of proteomic analysis of endometriosis to identify different protein expression in patients with endometriosis versus normal controls

Zhang, Hong; Niu, Yulan; Feng, Jie; Guo, Haipeng; Xue, Yu; Cui, Heng

doi:10.1016/j.fertnstert.2006.01.028

Cited by 90 publications

(93 citation statements)

References 36 publications

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“…It is known that in this disease several genes are expressed in aberrant forms, affecting cell proliferation and cell death in eutopic endometrium, which might favour the ectopic endometrial cell survival that may be involved in its aetiology (Johnson et al 2005, Zhang et al 2006. Substantial evidence links NFKB to antiapoptotic and proapoptotic effects, through the BCL2 family and the FAS/FASLG complex (Lee et al 1999, Pahl 1999, Wang et al 1999, Harada et al 2004.…”

Section: Discussionmentioning

confidence: 99%

“…This evidence suggests that IL6 may play a regulatory role on endometrial homeostasis through proapoptotic mechanisms in the peri-menses stage coincident with their maximal expression. This function in endometrial cell viability may be negatively affected in endometriosis, as indicated by the local reduction of IL6 levels at the late secretory phase concomitant with the decrease in the apoptosis rate during the same phase (Johnson et al 2005, Zhang et al 2006.…”

Section: Discussionmentioning

confidence: 99%

“…Differential gene expression is found by transcriptome or proteome studies in eutopic endometrium from women with and without endometriosis during the implantation window (Kao et al 2003), the secretory phase (Zhang et al 2006) or the late secretory endometrium (day 23-26; Sherwin et al 2008). The altered genes found did not coincide in these three studies using different methodologies, showing that endometriosis is a multi-factorial disease with a wide clinical spectrum of patients that included wide pain and subfertility ranges, beside the dynamic and rapid changes experienced by the endometrium throughout the secretory phase of the menstrual cycle.…”

Section: Discussionmentioning

confidence: 99%

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Nuclear factor κB pathway and interleukin-6 are affected in eutopic endometrium of women with endometriosis

Ponce¹,

Torres²,

Galleguillos³

et al. 2009

Reproduction

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In order to investigate the role of the nuclear factor kB (NFKB) pathway on gene expression in the eutopic endometrium in endometriosis, and in particular of interleukin-6 (IL6), we evaluated RELA, IkB kinase (CHUK), NFKBIA and IL6 expressions and NFKB DNA binding in eutopic endometrium from women with endometriosis. Eutopic endometrium was obtained from 37 women with endometriosis and 42 fertile women during laparoscopy. We analysed RELA, CHUK, NFKBIA and IL6 mRNA levels (RT-PCR); RELA, CHUK and NFKBIA proteins and p-NFKBIA/NFKBIA ratio (western blot); and NFKB binding (DNA shift assay) and IL6 concentration (ELISA) in endometrial explants. Our results indicate that mRNA and cytoplasmic proteins of RELA and CHUK exhibit constant levels in normal endometrium during the menstrual cycle. A dramatic increase (P!0.05) in NFKBIA mRNA expression, RELA nuclear presence and the mRNA and the protein of IL6 during late secretory phase was also observed in this tissue. By contrast, in eutopic endometrium from endometriosis patients, a decrease (P!0.05) in IL6 mRNA and protein (61%), NFKBIA mRNA (46%), p-NFKBIA/NFKBIA ratio (42%), RELA nuclear stromal (68%) and CHUK (48%) proteins were found exclusively during the late secretory phase compared with normal endometrium. In conclusion, the canonical activation of NFKB pathway is deregulated and may have reduced transcriptional function affecting NFKBIA and IL6 expression, genes related local proinflammatory processes. These molecular alterations observed during the late secretory phase in eutopic endometrium from endometriosis patients constitute a NFKB system dysfunction, suggesting that NFKB could be an important factor in endometriosis aetiology.

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Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

See 1 more Smart Citation

Nuclear factor κB pathway and interleukin-6 are affected in eutopic endometrium of women with endometriosis

Ponce¹,

Torres²,

Galleguillos³

et al. 2009

Reproduction

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“…The molecular events of EMT include down-regulation of epithelial markers (e.g., E-cadherin) and overexpression of mesenchymal markers (e.g., fibronectin and vimentin), which involves activation of a number of transcription factors, including Snail, Slug, Twist, Zeb1, and SIP1. Existing evidences have showed invasive behavior and cytoskeletal rearrangement of endometrial epithelial cells during ectopic implantation concomitant with reduced expression of E-cadherin (6) and up-regulation of vimentin expression (7) in endometriotic lesions compared with normal uterine endometrium. These data implicate a possible role of EMT in adenomyosis development.…”

mentioning

confidence: 99%

Proteomics Identification of Annexin A2 as a Key Mediator in the Metastasis and Proangiogenesis of Endometrial Cells in Human Adenomyosis

Zhou

et al. 2012

Molecular & Cellular Proteomics

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Adenomyosis is a common estrogen-dependent disorder of females characterized by a downward extension of the endometrium into the uterine myometrium and neovascularization in ectopic lesions. It accounts for chronic pelvic pain, dysmenorrhea, menorrhagia, and infertility in 8.8 -61.5% women worldwide. However, the molecular mechanisms for adenomyosis development remain poorly elucidated. Here, we utilized a two-dimensional polyacrylamide gel electrophoresis/MS-based proteomics analysis to compare and identify differentially expressed proteins in matched ectopic and eutopic endometrium of adenomyosis patients. A total of 93 significantly altered proteins were identified by tandem MS analysis. Further cluster analysis revealed a group of estrogen-responsive proteins as dysregulated in adenomyosis, among which annexin A2, a member of annexin family proteins, was found up-regulated most significantly in the ectopic endometrium of adenomyosis compared with its eutopic counterpart. Overexpression of ANXA2 was validated in ectopic lesions of human adenomyosis and was found to be tightly correlated with markers of epithelial to mesenchymal transition and dysmenorrhea severity of adenomyosis patients. Functional analysis demonstrated that estrogen could remarkably up-regulate ANXA2 and induce epithelial to mesenchymal transition in an in vitro adenomyosis model. Enforced expression of ANXA2 could mediate phenotypic mesenchymal-like cellular changes, with structural and functional alterations in a ␤-catenin/T-cell factor (Tcf) signaling-associated manner, which could be reversed by inhibition of ANXA2 expression. We also proved that enforced expression of ANXA2 enhanced the proangiogenic capacity of adenomyotic endometrial cells through HIF-1␣/VEGF-A pathway. In vivo, we demonstrated that ANXA2 inhibition abrogated endometrial tissue growth, metastasis, and angiogenesis in an adenomyosis nude mice model and significantly alleviated hyperalgesia. Taken together, our data unraveled a dual role for ANXA2 in the pathogenesis of human adenomyosis through conferring endometrial cells both metastatic potential and proangiogenic capacity, which could serve as a potential therapeutic target for the treatment of adenomyosis patients.

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“…Over the past 20 years, numerous studies on the pathogenesis and pathophysiology of endometriosis have increased our understanding of the role of steroid hormones, genetics, environment, immune system, and peripheral and central nervous system on the deployment, progression/impediments, signs, symptoms, and complications of the disease (7). Recently, the role of nutrition in maintenances as well as development of endometriosis has been considered as a matter of interest, which is mainly due to the physiologic and pathologic processes associated with the disease, such as inflammation, estrogen activity, menstrual cycles, organochlorines, and metabolism of prostaglandin that can be affected by diet.…”

Section: Contextmentioning

confidence: 99%

Diet and Risk of Endometriosis: A Systematic Review and Meta-Analysis Study

Hoorsan

Mirmiran

Chaichian

et al. 2017

Iran Red Crescent Med J

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Context: Given the role of nutrition and food, as well as environmental pollutants in the probability of developing endometriosis, this study aimed to identify the risk factors and preventive factors in a systematic review and meta-analysis.Evidence Acquisition: In this study, available references were searched for the relevant articles published "between" 2000 -2015. Among a total of 357 articles extracted from primary studies, 5 articles were finally selected for meta-analysis, which were ultimately analyzed for the effect of dietary factors and risk of endometriosis on 73,102 cases (2,608 cases and 70,494 controls). Conclusions: Evidence shows a need to improve the understanding of the impact of dietary components on the risk of endometriosis to modify and prevent this disease. Futhermore, more studies are required and recommended to clarify the role of diet in the incidence and progression of endometriosis.

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Use of proteomic analysis of endometriosis to identify different protein expression in patients with endometriosis versus normal controls

Cited by 90 publications

References 36 publications

Nuclear factor κB pathway and interleukin-6 are affected in eutopic endometrium of women with endometriosis

Nuclear factor κB pathway and interleukin-6 are affected in eutopic endometrium of women with endometriosis

Proteomics Identification of Annexin A2 as a Key Mediator in the Metastasis and Proangiogenesis of Endometrial Cells in Human Adenomyosis

Diet and Risk of Endometriosis: A Systematic Review and Meta-Analysis Study

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