Use of pulsed electric field permeabilization to extract astaxanthin from the Nordic microalga Haematococcus pluvialis

Martínez, Juan Manuel; Gojkovic, Živan; Ferro, Lorenza; Maza, Marcos; Álvarez, Ignacio; Raso, Javier; Funk, Christiane

doi:10.1016/j.biortech.2019.121694

Cited by 87 publications

(42 citation statements)

References 48 publications

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“…By evaluating the incubation process in the buffer solution, it was shown that ethanol was effective for extracting carotenoids from PEF-treated cells of X. dendrorhous ATCC 74219. Recently, several authors have reported that an aqueous incubation period after PEF treatment increased the extraction yield of carotenoids, lipids and proteins from microalgae and yeasts ( Luengo et al, 2014 ; Dimopoulos et al, 2018 ; Martínez et al, 2018 , 2019 ; Silve et al, 2018 ; Scherer et al, 2019 ). This improvement in the extraction has been attributed to enzyme-driven processes triggered by PEF that occur during the aqueous incubation period.…”

Section: Discussionmentioning

confidence: 99%

“…Furthermore, most carotenoids extraction techniques require the drying of biomass with subsequent extraction with a large amount of organic solvent. Therefore, it is desirable to develop efficient extraction methods from wet biomass using green solvents in order to achieve economical and eco-friendly processes (Martínez et al, 2019).…”

Section: Introductionmentioning

confidence: 99%

“…Several studies have demonstrated that electroporation by PEF improve the extraction of intracellular components such as proteins, lipids, and pigments from microorganisms has been reported ( Ganeva et al, 2003 ; Chittapun et al, 2020 ; Leonhardt et al, 2020 ). Very recently, it has been proposed that PEF not only permeabilized the cytoplasmatic membrane to enable the release of intracellular biomolecules but also trigger some enzymatic process that accelerates the autolysis and the subsequent release of intracellular components ( Martínez et al, 2018 , 2019 ; Silve et al, 2018 ; Scherer et al, 2019 ). The application of PEF technology on the recovery of intra-cellular carotenoids from X. dendrorhous has not been reported.…”

Section: Introductionmentioning

confidence: 99%

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Enzymatic Processes Triggered by PEF for Astaxanthin Extraction From Xanthophyllomyces dendrorhous

Aguilar-Machado

Delso

Martínez

et al. 2020

Front. Bioeng. Biotechnol.

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The aim of this study was to evaluate the potential of pulsed electric fields (PEF) to improve the extraction of the lipid-soluble astaxanthin from fresh biomass of a wild-type (CECT 11028) and mutant (ATCC 74219) Xanthophyllomyces dendrorhous strain using ethanol as solvent. Inactivation and propidium uptake studies revealed that inactivation is a good index for estimated the proportion of irreversible permeabilized cells when inactivation is higher than 70% in the two strains. Ethanol was ineffective for extracting carotenoids from the PEF-treated cells (20 kV/cm, 135 µs) of the two strains. However, after aqueous incubation of PEF-treated X. dendrorhous ATCC 74219 cells for 12 h, up to 2.4 ± 0.05 mg/g dried weight (d.w.) of carotenoids were extracted in ethanol. From total carotenoid extracted, around 84% corresponded to all-trans astaxanthin. The detection and quantification of esterase activity in the supernatant and the relationship between the percentage of esterase activity quantified and the amount of carotenoids extracted indicate that the extraction of astaxanthin was mediated by enzymatic esterase activity triggered by PEF during incubation. On the other hand, the formation of a large lipid globule into the cytoplasm of PEF-treated X. dendrorhous CECT 11028 cells during aqueous incubation prevented carotenoid extraction. The process developed in this investigation represents a more sustainable and greener method that those previously used for extracting astaxanthin from yeast.

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Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Enzymatic Processes Triggered by PEF for Astaxanthin Extraction From Xanthophyllomyces dendrorhous

Aguilar-Machado

Delso

Martínez

et al. 2020

Front. Bioeng. Biotechnol.

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“…The energy consumption of PEF is much lower than in those conventional techniques (Carullo et al, 2018;de Boer, Moheimani, Borowitzka, & Bahri, 2012). In addition, regarding nonpolar compounds recovery from microalgae, which constitute a promising source of these metabolites, PEF treatment followed by autolysis induction during aqueous incubation permits to substitute the injurious organic chemicals by more safe and ecofriendly solvents (Martínez, Gojkovic et al, 2019c).…”

Section: Pef-assisted Extraction From Microalgaementioning

confidence: 99%

Pulsed electric field‐assisted extraction of valuable compounds from microorganisms

Martínez

Delso

Álvarez

et al. 2020

Comp Rev Food Sci Food Safe

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138

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Microorganisms (bacteria, yeast, and microalgae) are a promising resource for products of high value such as nutrients, pigments, and enzymes. The majority of these compounds of interest remain inside the cell, thus making it necessary to extract and purify them before use. This review presents the challenges and opportunities in the production of these compounds, the microbial structure and the location of target compounds in the cells, the different procedures proposed for improving extraction of these compounds, and pulsed electric field (PEF)‐assisted extraction as alternative to these procedures. PEF is a nonthermal technology that produces a precise action on the cytoplasmic membrane improving the selective release of intracellular compounds while avoiding undesirable consequences of heating on the characteristics and purity of the extracts. PEF pretreatment with low energetic requirements allows for high extraction yields. However, PEF parameters should be tailored to each microbial cell, according to their structure, size, and other factors affecting efficiency. Furthermore, the recent discovery of the triggering effect of enzymatic activity during cell incubation after electroporation opens up the possibility of new implementations of PEF for the recovery of compounds that are bounded or assembled in structures. Similarly, PEF parameters and suspension storage conditions need to be optimized to reach the desired effect. PEF can be applied in continuous flow and is adaptable to industrial equipment, making it feasible for scale‐up to large processing capacities.

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“…In both PEF pre-treatments that we applied, the specific energy input, pulse duration and frequency remained constant at 100 kJ/kg, 100 ms and 2 Hz, whereas the field strength and the number of pulses varied in the different treatments. The minimum electric field strength necessary to produce changes in cells is 1 kV/cm, and it has been found that with a pulse duration of milliseconds an electric field of 3–4 kV/cm is enough to create electroporation [ 31 , 33 ]. Based on these data, we decided to compare a low electric field (1 kV/cm) with a moderate one (3 kV/cm).…”

Section: Methodsmentioning

confidence: 99%

Improved Extraction Efficiency of Antioxidant Bioactive Compounds from Tetraselmis chuii and Phaedoactylum tricornutum Using Pulsed Electric Fields

et al. 2020

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Pulsed electric fields (PEF) is a promising technology that allows the selective extraction of high-added value compounds by electroporation. Thus, PEF provides numerous opportunities for the energy efficient isolation of valuable microalgal bioactive substances (i.e., pigments and polyphenols). The efficiency of PEF-assisted extraction combined with aqueous or dimethyl sulfoxide (DMSO) solvents in recovering pigments and polyphenols from microalgae Tetraselmis chuii (T. chuii) and Phaeodactylum tricornutum (P. tricornutum) was evaluated. Two PEF treatments were applied: (1 kV/cm/400 pulses, 3 kV/cm/45 pulses), with a specific energy input of 100 kJ/kg. The total antioxidant capacity (TAC) was positively influenced by the use of DMSO. The highest TAC in the T. chuii culture was achieved at a lower extraction time and electric field than for P. tricornutum. The use of DMSO only improved the polyphenols′ extraction for P. tricornutum, whereas the PEF and extraction time were more important for T. chuii. Carotenoids and chlorophyll a were more efficiently extracted using DMSO, while chlorophyll b levels were higher following aqueous extraction for both microalgae. In P. tricornutum, the TAC and pigment extraction efficiency were in general higher at lower extraction times. It can be concluded that PEF may be a promising alternative for the enhancement of the selective extraction of antioxidant bioactive compounds from microalgae.

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Use of pulsed electric field permeabilization to extract astaxanthin from the Nordic microalga Haematococcus pluvialis

Cited by 87 publications

References 48 publications

Enzymatic Processes Triggered by PEF for Astaxanthin Extraction From Xanthophyllomyces dendrorhous

Enzymatic Processes Triggered by PEF for Astaxanthin Extraction From Xanthophyllomyces dendrorhous

Pulsed electric field‐assisted extraction of valuable compounds from microorganisms

Improved Extraction Efficiency of Antioxidant Bioactive Compounds from Tetraselmis chuii and Phaedoactylum tricornutum Using Pulsed Electric Fields

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