Use of quasiline luminescence spectra for quantitative determination of several polycyclic hydrocarbons

Application of the Shpol'skii effect to quantitative analysis of monomethylphenanthrene isomers

“…To a degree difficult to estimate it is also due to the reabsorption effect in the (0-0) transition, which obviously also limits the linearity range of others aromatics (24).…”

“…Having provided such optimization of the experimental conditions, quantitative measurements have been attempted either by reference to a calibration curve (23) or by the method of standard addition (8, 23). In the cases where linearity of the fluorescence intensity is observed for a limited range of concentration, the latter method has been found to be the most convenient procedure (24). It has been employed successfully only for a limited number of quantitative analyses dealing mainly with the determination of benzo[a]pyrene (8), dimethylbenzo[a] anthracene or 7,12-dimethylbenzanthracene (25).…”

“…It has been employed successfully only for a limited number of quantitative analyses dealing mainly with the determination of benzo[a]pyrene (8), dimethylbenzo[a] anthracene or 7,12-dimethylbenzanthracene (25). The fluorescence spectra of these PAH are easily distinguishable even at 77 K and negligible mutual perturbation was found to affect the quantitative results even in a complex mixture of PAH (24,26). A different situation arises when the analysis concerns closely related compounds whose spectra axe strongly overlapping.…”

Low-temperature fluorescence spectrometric determination of polycyclic aromatic hydrocarbons by matrix isolation

“…It is now apparent that Shpol'skii spectra can be very useful for "fingerprinting" specific compounds present at very low levels in mixtures of PAH (6)(7)(8)(9)(10)(11)(12)(13)(14). Quantitative analyses of PAH by Shpol'skii spectroscopy have also been reported (7,(10)(11)(12)(15)(16)(17)(18)(19)(20)(21). However, a number of difficulties can be encountered in performing precise quantitative analyses in Shpol'skii matrices (22,23).…”

“…Bandwidths in Shpol'skii spectra are in certain cases dependent upon the fluorophore concentration (27,31). In most cases in which analytical working curves have been reported for Shpol'skii matrices, the linear region is restricted, even when careful standardization (usually a combination of standard addition and internal standard methods (10,11,15,16)) is employed. There appears to be only one published report (10) in which a linear working region exceeding three decades in concentration has been claimed for Shpol'skii fluorescence; other quantitative studies (7,11,15,18,20) have demonstrated a very restricted linear working range (2.5 decades or less).…”