Use of Screen‐printed Electrodes Modified by Prussian Blue and Analogues in Sensing of Cysteine

Bonacin, Juliano Alves; Santos, Pãmyla L. dos; Katic, Vera; Foster, Christopher W.; Banks, Craig E.

doi:10.1002/elan.201700628

Cited by 33 publications

(25 citation statements)

References 36 publications

(41 reference statements)

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“…ferrocyanide is also possible and may be desirable when a less acidic pH is required to preserve the activity of enzymes or other biomolecules. [15] Chemical growth, on the other hand, may also be used to produce Prussian Blue films, both on electrodes [26,30,31] and on inert surfaces [32] in many different ways. [33] However, this is not an ideal approach because it lacks the surface selectivity and much of the control that electrochemical methods enjoy.…”

Section: Preparation Of Pbmentioning

confidence: 99%

Electrochromic biosensors based on screen-printed Prussian Blue electrodes

Pellitero

Fremeau

Villa

et al. 2019

Sensors and Actuators B: Chemical

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Highlights We present the production of an electrochromic screen-printable paste based on Prussian Blue. These blue electrodes display excellent spectroelectrochemical reversibility and high catalytic activity towards the reduction of H2O2.  These blue electrodes enable both the electrochemical and optical quantitative determination of hydrogen peroxide.  The reported blue electrodes are highly suitable transducers for the construction of oxidase-based biosensors.  A glucose biosensor is presented to demonstrate the spectroelectrochemical capabilities of these new electrodes. AbstractPrussian Blue (PB)-modified graphite screen-printed electrodes are increasingly being used in electrochemical biosensors. However, they do not allow the observation of the electrochromism of PB. This work presents the construction of PB-based, electrochromic screen-printed biosensors. Although electrically more resistive than their graphite counterparts, these new PBbased electrodes enable both the amperometric and colorimetric detection of hydrogen peroxide.This is the first time that this has been achieved using screen-printed electrodes, and we demonstrate it spectroelectrochemically on a glucose biosensor. The biosensor electrochemical performance equals that of previously reported PB/graphite electrodes, being able to detect down to 4 µM H2O2 and 54 µM glucose. At the same time, and in contrast to PB/graphite electrodes, the new PB-based electrodes afford the optical detection of these two analytes down to 1.2 µM and 15 µM, respectively. The dynamic ranges of the glucose biosensors obtained at the PBbased electrodes are 0.1-1 mM (amperometric) and 0.025-2.5 mM (Colorimetric), matching the physiological glucose concentration range in body fluids other than blood or serum.

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Section: Preparation Of Pbmentioning

confidence: 99%

Electrochromic biosensors based on screen-printed Prussian Blue electrodes

Pellitero

Fremeau

Villa

et al. 2019

Sensors and Actuators B: Chemical

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“…Cobalt Phthalocyanine, an organic semiconductor ensuring sensor stability and sensitivity is another modifier used in the development of CMEs [44][45][46][47]. Due to its chemical composition, Prussian Blue has very good electrochemical properties, being most frequently used in the manufacture of electrochemical (bio)sensors detecting hydrogen peroxide [48][49][50][51][52][53][54][55][56]. These electroactive materials could be used for the development of novel electrochemical sensors for different analysts of great importance such is Phe.…”

Section: Introductionmentioning

confidence: 99%

Voltammetric Determination of Phenylalanine Using Chemically Modified Screen-Printed Based Sensors

Dinu

Apetrei

2020

Chemosensors

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This paper describes the sensitive properties of screen-printed carbon electrodes (SPCE) modified by using three different electroactive chemical compounds: Meldola’s Blue, Cobalt Phthalocyanine and Prussian Blue, respectively. It was demonstrated that the Prussian Blue (PB) modified SPCE presented electrochemical signals with the highest performances in terms of electrochemical process kinetics and sensitivity in all the solutions analyzed. PB-SPCE was demonstrated to detect Phe through the influence it exerts on the redox processes of PB. The PB-SPCE calibration have shown a linearity range of 0.33–14.5 µM, a detection limit (LOD) of 1.23 × 10−8 M and the standard deviation relative to 3%. The PB-SPCE sensor was used to determine Phe by means of calibration and standard addition techniques on pure samples, on simple pharmaceutical samples or on multicomponent pharmaceutical samples. Direct determination of the concentration of 4 × 10−6–5 × 10−5 M Phe in KCl solution showed that the analytical recovery falls in the range of 99.75–100.28%, and relative standard deviations in the range of 2.28–3.02%. The sensors were successfully applied to determine the Phe in pharmaceuticals. The validation of the method was performed by using the FTIR, and by comparing the results obtained by PB-SPCE in the analysis of three pharmaceutical products of different concentrations with those indicated by the producer.

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“…This high sensibility of the PB/SPCE was due to the changes in the sensors’ surface, which promoted the transfer of electrons and the accumulation or diffusion of analytes. The literature provides numerous studies in which the modified sensors with Prussian Blue present better characteristic and better results in the detection of antioxidant compounds [ 40 ].…”

Section: Resultsmentioning

confidence: 99%

“…In the case of SPCE, an anodic and a cathodic peak were observed, due to the redox process of diosmin [ 33 ]. In the case of PB/SPCE, the peaks which were observed corresponded to the overlap of the redox processes of diosmin and PB ( Figure 4 b) [ 40 ]. It could be noticed that in the case of PB/SPCE, the peaks had higher currents and lower E 1/2 .…”

Section: Resultsmentioning

confidence: 99%

Determination of Diosmin in Pharmaceutical Products with Chemically Modified Voltammetric Sensors

Gunache

Apetrei

2021

IJMS

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In this paper, the electrochemical behavior of two types of sensors based on modified screen-printed electrodes (one screen-printed electrode based on carbon (SPCE) and another screen-printed electrode modified with Prussian Blue (PB/SPCE)) was studied with the aim of sensitive detection of diosmin, an active pharmaceutical compound from the class of flavonoids. The scan electron microscopy technique was used for the morphological characterization of PB/SPCE. The preliminary analysis assessed the electrochemical behavior of SPCE and PB/SPCE in KCl solution and in a double solution of potassium ferrocyanide–potassium chloride. It was shown that the active area of PB/SPCE is superior to the one of SPCE, the greater sensitivity being related with the presence of the electroactive modifier. Similarly, in the case of diosmin detection, the PB/SPCE sensor detect more sensitivity the diosmin due to the electrocatalytic effect of PB. From the study of the influence of reaction rate on the sensor’s electrochemical response, it was shown that the detection process is controlled by the adsorption process, the degree of surface coverage with electroactive molecules being higher in the case of PB/SPCE. From the PB/SPCE calibration curve, it wasdetermined that it has high sensitivity and low detection and quantification limit values (limit of detection 5.22 × 10−8 M). The applicability of the PB/SPCE sensor was confirmed by sensitive analysis of diosmin in pharmaceutical products. The voltammetric method is suitable for the detection and quantification of diosmin in pharmaceutical products. The method is simple, accurate, and quick and can be used in routine analysis in the examination of the quality of pharmaceutical products and other types of samples.

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Use of Screen‐printed Electrodes Modified by Prussian Blue and Analogues in Sensing of Cysteine

Cited by 33 publications

References 36 publications

Electrochromic biosensors based on screen-printed Prussian Blue electrodes

Electrochromic biosensors based on screen-printed Prussian Blue electrodes

Voltammetric Determination of Phenylalanine Using Chemically Modified Screen-Printed Based Sensors

Determination of Diosmin in Pharmaceutical Products with Chemically Modified Voltammetric Sensors

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