Use of short microsatellites from database sequences to generate polymorphisms among Lycopersicon esculentum cultivars and accessions of other Lycopersicon species

Abstract

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A new integrated genetic linkage map of the soybean

“…The expansion or contraction of dinucleotide repeat length in exons may likely be suppressed due to the deterious nature of the frame-shift mutation that would frequently result in translated regions. Microsatellite markers derived from repeat arrays in genes are reported to be significantly less polymorphic than markers generated from longer arrays (Smulders et al 1997). Other factors such as selection against large alteration in coding DNA or even a closely associated sequence that may play a role in gene expression could constrain microsatellite expansion or contraction.…”

Transferability and characterization of microsatellite markers in five Bromeliaceae species belonging to the subfamilies Pitcairnioideae and Bromelioideae

“…SSR markers can be transferred between related species and genera, which considerably reduces their costs (Ferreira & Grattaplaglia 1998). SSR transferability has been reported to many families of plants, including Fabaceaea (Peakall et al 1998, Kölliker et al 2001) Cucurbitaceae (Katzir et al 1996), Poaceae (Saghai Maroof et al 1994, Röder et al 1995, Thiel et al 2003, Solanaceae , Smulders et al 1997, Nagy et al 2007, Moon et al 2008; Euphorbiaceae (Yu et al 2011) and Bromeliaceae (Sarthou et al 2003, Paggi et al 2008. Thus, in this paper eight microsatellite markers previously developed for Pitcairnia albiflos and five markers developed for Pitcairnia geysksii were used in cross-amplification tests of five other neotropical Bromeliaceae species (Pitcairnia flammea, Aechmea nudicaulis, Aechmea ramosa, Billbergia horrida and Billbergia euphemiae).…”

Análisis de la variabilidad en las especies del subgénero Eulycopersicon más relacionadas con el tomate cultivado.

“…Este proceso de difusión sometió al tomate a una fuerte presión de selección, provocando el fenómeno genético conocido como "cuello de botella", que redujo extraordinariamente la variación genética de esta hortaliza (Rick, 1976;Rick y Yoder, 1978). Este hecho queda demostrado por la escasa presencia de polimorfismo en los cultivares europeos, respecto a los regionales de Sur América, Centro América y México (Miller y Tanksley, 1990;Williams y St. Clair, 1993;Smulders et al, 1997;Bredemeijer et al, 1998).…”

“…Esta variabilidad está fuertemente asociada a su localización geográfica, pudiéndose encontrar gradualmente en clinas. La existencia de esta variabilidad en las estructuras florales tiene implicaciones en los procedimientos de muestreo así como en el mantenimiento de las colecciones, sirviendo ciertas metodologías para unas determinadas zonas, mientras que para otras no son validas (Widrlechener, 1987 Los primeros estudios con microsatélites en tomate se hicieron para comprobar su capacidad en la identificación de cultivares de tomate (Vosman et al, 1992;Smulders et al, 1997), consiguiendo diferenciar 15 cultivares antiguos. Se comprobó además la segregación mendeliana del locus (GATA) 4 .…”