Use of specific bacteria for the determination of mutagenic and carcinogenic compounds

Abstract

Possibilities of the use of Escherichia coll K-12 In the determination of mutagenic compounds have been tested. Analytical parameters, e.g., the range of linearity of the analytical curve, the detection limit and the sensitivity, the accuracy, and the precision of analysis, have been evaluated for a series of nitrofurans. The detection limit Is In the range of 10~7-10~* mol L~\ Analyses of real samples (determination of 5-nHro-2-furylacryllc acid In wine and nitrovin In chicken meat) have been completed without any preseparation.

Hybrid biosensor for the determination of lactose

“…A genotoxic agent that induces cell filamentation (an SOS function) (24) also induces the production of /3-galactosidase. The amount of produced enzyme is dependent on the type of genotoxin and on its concentration (14,17). Among many genotoxic compounds nitrovin was found to be the best inductor of /3-galactosidase production in E. coli cells (17), and therefore it was used for induction of /3-galactosidase.…”

“…The amount of produced enzyme is dependent on the type of genotoxin and on its concentration (14,17). Among many genotoxic compounds nitrovin was found to be the best inductor of /3-galactosidase production in E. coli cells (17), and therefore it was used for induction of /3-galactosidase. The inductor at a certain concentration may inhibit protein synthesis, which would lead to the lowering of /3-galactosidase activity.…”

“…Optical fibers are becoming increasingly popular as couriers of information in analytical detection systems. They have been used in fluorescence detection or excitation in a variety of configurations (15)(16)(17)(18) and for remote acquisition of spectroelectrochemical data (19). They have also been used in a variety of sensors, including pH sensors (20), luminescence-based biosensors (21,22), and affinity/fluorescence sensors (23).…”