Use of SSU rDNA group-I intron to distinguish Monilinia fructicola from M. laxa and M. fructigena

Fulton, C. E.

doi:10.1016/s0378-1097(97)00492-8

Cited by 53 publications

(32 citation statements)

References 0 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…Alternaria alternata is a ubiquitous environmental saprophyte which has been associated with allergic disorders and ocular infections (25,63). Monilinia laxa has been described as a causal agent of brown rot of stone fruits (21). The form genus Fusarium includes a variety of saprophytes, plant pathogens, human pathogens, and mycotoxin producers (18,45,53).…”

Section: Discussionmentioning

confidence: 99%

Abundant and Diverse Fungal Microbiota in the Murine Intestine

Scupham

Presley

Wei

et al. 2006

Appl Environ Microbiol

159

131

View full text Add to dashboard Cite

Enteric microbiota play a variety of roles in intestinal health and disease. While bacteria in the intestine have been broadly characterized, little is known about the abundance or diversity of enteric fungi. This study utilized a culture-independent method termed oligonucleotide fingerprinting of rRNA genes (OFRG) to describe the compositions of fungal and bacterial rRNA genes from small and large intestines (tissue and luminal contents) of restricted-flora and specific-pathogen-free mice. OFRG analysis identified rRNA genes from all four major fungal phyla: Ascomycota, Basidiomycota, Chytridiomycota, and Zygomycota. The largest assemblages of fungal rRNA sequences were related to the genera Acremonium, Monilinia, Fusarium, Cryptococcus/Filobasidium, Scleroderma, Catenomyces, Spizellomyces, Neocallimastix, Powellomyces, Entophlyctis, Mortierella, and Smittium and the order Mucorales. The majority of bacterial rRNA gene clones were affiliated with the taxa Bacteroidetes, Firmicutes, Acinetobacter, and Lactobacillus. Sequence-selective PCR analyses also detected several of these bacterial and fungal rRNA genes in the mouse chow. Fluorescence in situ hybridization analysis with a fungal small-subunit rRNA probe revealed morphologically diverse microorganisms resident in the mucus biofilm adjacent to the cecal and proximal colonic epithelium. Hybridizing organisms comprised about 2% of the DAPI (4,6-diamidino-2-phenylindole, dihydrochloride)-positive organisms in the mucus biofilm, but their abundance in fecal material may be much lower. These data indicate that diverse fungal taxa are present in the intestinal microbial community. Their abundance suggests that they may play significant roles in enteric microbial functions.

show abstract

Section: Discussionmentioning

confidence: 99%

Abundant and Diverse Fungal Microbiota in the Murine Intestine

Scupham

Presley

Wei

et al. 2006

Appl Environ Microbiol

159

131

View full text Add to dashboard Cite

show abstract

“…Fulton and Brown (1997) located a group I intron in the ribosomal small subunit (SSU) gene of M. fructicola. Ioos and Frey (2000) developed a rapid identification method of the Monilinia species in a single PCR-run.…”

mentioning

confidence: 99%

Characterization of M. laxa and M. fructigena isolates from Hungary with MP-PCR

Szôdi

Komjáti

Turóczi

2012

Hort. Sci. (Prague)

View full text Add to dashboard Cite

Monilinia laxa (Monilia laxa), Monilinia fructicola (Monilia fructicola) and Monilinia fructigena (Monilia fructigena) are the causal agents of brown rot on pome and stone fruits in Hungary. Forty-five isolates collected from different hosts, different years in several orchards were used for characterization of the M. laxa and M. fructigena population in Hungary. The isolates were identified on species level based on morphological and molecular biological methods; out of these 24 were M. laxa, 20 were M. fructigena and 1 was M. fructicola. Populations of the three Monilinia species were studied with microsatellite primers and the degree of genetic diversity within the species was measured. The population structure analysis revealed that genetic diversity within M. laxa subpopulations was H S = 0.1599, while within M. fructigena subpopulations was H S = 0.2551. The total genetic diversity was H T = 0.3846, while genetic diversity between M. laxa and M. fructigena subpopulations was D ST = 0.1771. No clustering relationship was observed among isolates by the different years or hosts.

show abstract

“…The presence of group I introns is quite common in many fungal species (Fulton & Brown, 1997, Nishida et al, 1993, DePriest & Been, 1992) and they are characterized by conserved core elements and secondary structure (Cech, 1988). They also show a high degree of variability in terms of size, number of insertions and location of insertion (Gargas et al, 1995, Oliveira et al, 1994Nishida et al, 1993).…”

Section: ;mentioning

confidence: 99%

Intron polymorphism in small subunit rDNA of Nectria galligena

Crockard¹,

Fulton

Bjourson³

et al. 1998

Microbiology

View full text Add to dashboard Cite

PCR amplification of the small subunit (SSU) rDNA gene of 40 isolates of Nectria galligena revealed four length polymorphisms. PCR-RFLP analysis of the SSU rDNA gene divided the isolates into four categories similar, but not identical, to categories identified by Southem-RFLP analysis. Nucleotide sequence analysis revealed that isolates in three of the four SSU rDNA (18s) categories possess an intron of 363 bp, 1185 bp or 1423 bp at the NS 7 priming site. Isolates in the fourth category do not possess an intron. The nucleotide sequences of these introns did not contain the core elements characteristic of typical group I introns, nor did they exhibit a group I intron secondary structure. Homology between the introns indicates a common lineage, all three possibly having come from a larger intron and having been formed by subsequent deletions. PCR primers upstream of the SSU rDNA intron region and from within the internal transcribed spacer 1 region amplify a product specific to N. ga//igena, which will confirm the identity of the pathogen and reveal its 185 category in a single reaction.

show abstract

Use of SSU rDNA group-I intron to distinguish Monilinia fructicola from M. laxa and M. fructigena

Cited by 53 publications

References 0 publications

Abundant and Diverse Fungal Microbiota in the Murine Intestine

Abundant and Diverse Fungal Microbiota in the Murine Intestine

Characterization of M. laxa and M. fructigena isolates from Hungary with MP-PCR

Intron polymorphism in small subunit rDNA of Nectria galligena

Contact Info

Product

Resources

About