Use of Synthetic Medium in Study of Antibacterial Effect of Sulfathiazole.

Studies Pertaining to the Antibacterial Activity of Sulfathiazole and its Methyl Derivative

“…The present report represents a more detailed and extended presentation of experimental data pertaining to the antibacterial activity of the thiazole compounds and their possible mode of action than those previously published from this laboratory (Muir, Shamleffer, and Jones, 1940, 1941a, and 1941b. In the present instance it is our purpose (a) to show that synthetic media are distinctly superior to the conventionally employed peptone-containing media for such studies, (b) to present a detailed appraisal of the inhibitory effect of the thiazole drugs on cellular proliferation, (c) to demonstrate the drug-antagonistic effect of p-aminobenzoic acid and other substances in a strictly synthetic medium, and (d) to compare the antibacterial effect of the thiazole compounds as observed in vivo.…”

Metabolite Antagonists.

“…The molecular inhibition ratio between sulfanilamide and PABA was found to be 23,000:1. (84,196,222,231,245,299,302,389,405,427,431) (405) demonstrated the roughly linear relationship between the concentrations of sulfonamides (sulfanilamide, sulfapyridine, and sulfathiazole) and the minimum concentration of PABA required to prevent their bacteriostatic action (figure 1). They also noted that the concentration of PABA required to reverse the sulfonamides was directly related to their antibacterial activity.…”