Use of Synthetic Peptides in the Study of the Antibody Response to Plasmodium Vivax Sporozoites

Pessi, Antonello; Michel, Martine; Bianchi, Elisabetta; Mendis, A; Tougne, Chantal; As, Verdini; Ph, Lambert; Carter, R; Mendis, Kamini; G, Del Giudice

doi:10.4269/ajtmh.1990.42.17

Cited by 15 publications

(4 citation statements)

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“…Levels of antibodies against the different peptides of P. vivax CSP were measured in human and Aotus plasma samples by an ELISA (Pessi et al, 1990). Individual peptides were used to coat¯at-bottom ed wells in 96well plates (MaxiSorp; Nunc) at a concen- tration of 10 m g/ml in phosphate-buffered saline (PBS, pH 7.8).…”

Section: Elisamentioning

confidence: 99%

Mapping and comparison of the B cell epitopes recognized on the Plasmodium vivax circumsporozoite protein by immune Colombians and immunized Aotus monkeys

M.¹

1998

Annals of Tropical Medicine And Parasitology

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Plasma samples of individuals from two malaria-endemic villages on the Colombian Pacific coast and synthetic peptides representing different fragments of the central and flanking regions of the Plasmodium vivax circumsporozoite protein (CSP) were used to perform a fine mapping of the B-cell epitopes on the whole CSP. In addition, the immunogenicity of long polypeptides corresponding to the amino (N) and carboxyl (C) regions was evaluated in Aotus monkeys. The epitopes recognized after natural infection of humans and after immunization of Aotus with these synthetic peptides were compared. Human samples more frequently contained specific antibodies to the central region. The type-I repeat region of the CSP was predominantly recognized by the human sera (by 68% of those from the village of Zacarías and 75% of those from Bajo Calima), a significantly smaller population reacting with the type-II repeat (20% and 11%, respectively). Most of the sera reacting with the type-I repeat recognized the minimal epitope AGDR. Although the N- and C-terminal polypeptides were both highly immunogenic in Aotus and induced long-lasting antibodies, titres of antibodies to the C-terminal polypeptide were higher than those of antibodies to the N-terminal. Competitive inhibition assays performed using human and monkey plasma allowed the identification of dominant B-cell epitopes on sequence 71-90 (p8) from the amino region and sequence 332-361 (p24/p25) from the carboxyl region. The high prevalence of naturally induced antibodies to the three epitopes, the possible functional role of the corresponding sequences, and the high immunogenicity of these epitopes in Aotus could be of great importance in the development of a malaria vaccine based on P. vivax CSP.

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Section: Elisamentioning

confidence: 99%

Mapping and comparison of the B cell epitopes recognized on the Plasmodium vivax circumsporozoite protein by immune Colombians and immunized Aotus monkeys

M.¹

1998

Annals of Tropical Medicine And Parasitology

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“…The central region of the CSP, composed of tandemly repeated amino-acid sequences [8], is recognized by most antibodies against P. vivax sporozoites [9]. Recognition of epitopes in the P. vivax CSP by monoclonal antibodies [10], indicates that two repeats are essential. The results of immunogenicity studies on the non-repeat regions of P. vivax indicated the presence of B-and T-cell epitopes in this region [11].…”

Section: Introductionmentioning

confidence: 99%

Induction of mucosal and systemic humoral immune responses in murine system by intranasal immunization with peptide antigens of P. vivax and CpG oligodeoxynucleotide (ODN) in microparticle delivery

Bhat

Seth

Babu

et al. 2009

International Immunopharmacology

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“…Enzyme Linked Immunosorbent Assay (ELISA) -ELISA was performed according to Zavalla et al (1986), with some modifications, using synthetic peptides corresponding to repeats of the circumsporozoite antigen of the "classic" P. vivax (Pvc) (Pessi et al 1990) (DDAAD) 9 , P. vivax VK247 (Pvk) (Rosenberg et al 1989) (ANGAGNQPG) 4 and the human P. vivax-like (Pvl) ) (APGANQEGGAA) 3 . We also used a synthetic peptide corresponding to repeats of the circumsporozoite antigen of the P. malariae/P.…”

Section: Serological Testsmentioning

confidence: 99%

Antibodies anti Bloodstream and Circumsporozoite Antigens (Plasmodium vivax and Plasmodium malariae/P. brasilianum) in Areas of Very Low Malaria Endemicity in Brazil

Curado

Duarte

Lal

et al. 1997

Mem. Inst. Oswaldo Cruz

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Use of Synthetic Peptides in the Study of the Antibody Response to Plasmodium Vivax Sporozoites

Cited by 15 publications

References 0 publications

Mapping and comparison of the B cell epitopes recognized on the Plasmodium vivax circumsporozoite protein by immune Colombians and immunized Aotus monkeys

Mapping and comparison of the B cell epitopes recognized on the Plasmodium vivax circumsporozoite protein by immune Colombians and immunized Aotus monkeys

Induction of mucosal and systemic humoral immune responses in murine system by intranasal immunization with peptide antigens of P. vivax and CpG oligodeoxynucleotide (ODN) in microparticle delivery

Antibodies anti Bloodstream and Circumsporozoite Antigens (Plasmodium vivax and Plasmodium malariae/P. brasilianum) in Areas of Very Low Malaria Endemicity in Brazil

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