Use of the alkaline comet assay for industrial genotoxicity screening

Hartmann, Andreas; Elhajouji, Azeddine; Kiskinis, Evangelos; Poetter, Franziska; Martus, Hans-Joerg; Fjällman, A; Frieauff, Wilfried; Suter, Willi

doi:10.1016/s0278-6915(01)00031-x

Cited by 88 publications

(30 citation statements)

References 21 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…The alkaline comet and micronucleus have been the most commonly used assays for the screening of potential genotoxicants (MacGregor et al, 1987;Rojas et al, 1999;Hartmann et al, 2001) that utilize a variety of cell types. While the alkaline version of the comet assay (Singh et al, 1988) detects primary (repairable) single-and double-strand breaks and alkali-labile sites before and after DNA repair process, the micronucleus test detects DNA lesions after their fixation as chromosome mutations.…”

Section: Introductionmentioning

confidence: 99%

Absence of mutagenicity effects of Psidium cattleyanum Sabine (Myrtaceae) extract on peripheral blood and bone marrow cells of mice

Costa

Vieira

Andrade³

et al. 2008

Genet. Mol. Res.

View full text Add to dashboard Cite

AbSTrACT. Cattley guava (Psidium cattleyanum Sabine) is a native fruit of Brazil that is popular both as a sweet food and for its reputed therapeutic properties. We examined whether it could damage DNA using the alkaline single-cell gel electrophoresis (comet assay) and the micronucleus test in leukocytes and in bone marrow cells of mice. P. cattleyanum leaf extract was tested at concentrations of 1000, 1500 and 2000 mg/kg. N-nitroso-N-ethylurea was used as a positive control. Peripheral blood leukocytes were collected 4 and 24 h after the treatments for the comet assay, and bone marrow cells were collected after 24 and 48 h for the micronucleus test. Unlike N-nitroso-N-ethylurea, P. cattleyanum extract failed to induce a significant increase in cell DNA damage, in micronucleated cell frequency, and in bone marrow toxicity. The lack of mutagenicity and cytotoxicity with high doses of this plant extract means that it can be safely used in traditional medicine.

show abstract

Section: Introductionmentioning

confidence: 99%

Absence of mutagenicity effects of Psidium cattleyanum Sabine (Myrtaceae) extract on peripheral blood and bone marrow cells of mice

Costa

Vieira

Andrade³

et al. 2008

Genet. Mol. Res.

View full text Add to dashboard Cite

show abstract

“…The alkaline comet assay is increasingly used in industrial in vitro genotoxicity testing (Rojas et al, 1999;Hartmann et al, 2001) and has also been used an important tool to evaluate the genotoxic potential of compounds in vivo (Rojas et al, 1999;Sekihashi et al, 2002). Our comet assay results for peripheral blood leukocytes from blood taken from 12-day old female and male Swiss mice 4 h and 24 h after treatment are summarized in Table 1.…”

Section: Resultsmentioning

confidence: 99%

Evaluation of the in vivo mutagenic potential of hydroalcoholic extracts of the northern highbush blueberry (Vaccinium corymbosum L. Ericales, Ericaceae) on peripheral blood cells of Swiss mice (Mus musculus Rodentia, Muridae)

2008

View full text Add to dashboard Cite

The northern highbush blueberry (Vaccinium corymbosum L. Ericales, Ericaceae) is very rich in anthocyanins, natural pigments which have strong antioxidant properties and potential health benefits, resulting in the worldwide use the blueberry as a medicinal plant. We investigated the mutagenic potential of simple hydroalcoholic extracts of V. corymbosum acutely administrated by gavage to Swiss mice at doses of 1 g kg -1 , 1.5 g kg -1 and 2 g kg -1 . Peripheral blood cells were collected 4 h and 24 h post-gavage and assessed by the alkaline comet assay, with further blood samples being collected at 48 h and 72 h for assessment using the micronucleus (MN) assay. Our results show that the V. corymbosum extracts did not induce any statistically significant increase in the average amount of DNA damage in peripheral blood leukocytes. However, we did record a significant increase in the frequency of micronucleated polychromatic erythrocytes at the three doses tested.

show abstract

“…This assay has the advantages of requiring a small number of cells per sample, being amenable to almost all cell types whether actively dividing or quiescent, and being relatively inexpensive, since only basic equipment is required (Tice et al, 2000;Hartmann et al, 2001Hartmann et al, , 2003Brendler-Schwaab et al, 2005). In the comet assay, cells are embedded on agarose gel on a glass slide, lysed to break cell and nuclear membranes, and exposed to an alkaline solution to yield single-stranded DNA from released double-stranded DNA.…”

Section: Treatmentmentioning

confidence: 99%

Genotoxicity testing of Ambelania occidentalis (Apocynaceae) leaf extract in vivo

Castro

Perazzo²,

Maistro

2009

Genet. Mol. Res.

View full text Add to dashboard Cite

ABSTRACT. Ambelania occidentalis is routinely used in folk medicine for treating gastrointestinal disorders, even though there have been no safety trials. We evaluated the genotoxic potential of hydro-alcoholic extracts of this plant in mice; induced DNA damage was assessed in peripheral blood leukocytes and micronucleus induction was assessed in polychromatic erythrocytes from bone marrow. The extract was administered by an oral route at single doses of 1000, 1500 and 2000 mg/ kg body weight. N-nitroso-N-ethylurea was used as a positive control. The comet assay was performed on peripheral blood leukocytes at 4 and 24 h after treatment, and the micronucleus test was carried out on bone marrow cells collected at 24 and 48 h after treatment. The ratio of polychromatic/normochromatic erythrocytes was scored for cytotoxicity assessment. No increase in the number of micronucleated polychromatic erythrocytes from bone marrow or in leukocyte DNA damage was observed. The hydro-alcoholic extracts of A. occidentalis had no mutagenic or cytotoxic effects in the mouse cells.

show abstract

Use of the alkaline comet assay for industrial genotoxicity screening

Cited by 88 publications

References 21 publications

Absence of mutagenicity effects of Psidium cattleyanum Sabine (Myrtaceae) extract on peripheral blood and bone marrow cells of mice

Absence of mutagenicity effects of Psidium cattleyanum Sabine (Myrtaceae) extract on peripheral blood and bone marrow cells of mice

Evaluation of the in vivo mutagenic potential of hydroalcoholic extracts of the northern highbush blueberry (Vaccinium corymbosum L. Ericales, Ericaceae) on peripheral blood cells of Swiss mice (Mus musculus Rodentia, Muridae)

Genotoxicity testing of Ambelania occidentalis (Apocynaceae) leaf extract in vivo

Contact Info

Product

Resources

About