Use of the CDC Autocidal Gravid Ovitrap to Control and Prevent Outbreaks ofAedes aegypti(Diptera: Culicidae)

Abstract

Populations of Aedes aegypti (L.) can be managed through reductions in adult mosquito survival, number of offspring produced, or both. Direct adult mortality can be caused by the use of space sprays or residual insecticides to mosquito resting sites, and with a variety of residual insecticide-impregnated surfaces that are being tested, such as curtains, covers for water-storage vessels, bednets, and ovitraps. The fertility of Ae. aegypti populations can be reduced by the use of autocidal oviposition cups that prevent the development of mosquitoes inside the trap by mechanical means or larvicides, as well as by releasing sterile, transgenic, and para-transgenic mosquitoes. Survival and fertility can be simultaneously reduced by capturing gravid female Ae. aegypti with sticky gravid traps. We tested the effectiveness of the novel Centers for Disease Control and Prevention autocidal gravid ovitrap (CDC-AGO trap) to control natural populations of Ae. aegypti under field conditions in two isolated urban areas (reference vs. intervention areas) in southern Puerto Rico for 1 yr. There were significant reductions in the captures of female Ae. aegypti (53–70%) in the intervention area. The presence of three to four AGO control traps per home in 81% of the houses prevented outbreaks of Ae. aegypti, which would be expected after rains. Mosquito captures in BG-Sentinel and AGO traps were significantly and positively correlated, showing that AGO traps are useful and inexpensive mosquito surveillance devices. The use of AGO traps to manage Ae. aegypti populations is compatible with other control means such as source reduction, larviciding, adulticiding, sterile insect techniques, induced cytoplasmic incompatibility, and dominant lethal gene systems.

Assessment of synthetic floral-based attractants and sugar baits to capture male and female Aedes aegypti (Diptera: Culicidae)

“…aegypti . As for females, the use of simple gravid traps, such as the CDC Autocidal Gravid Trap and GAT [41, 42], and the use of BG-Sentinel traps (with and without CO 2 ) are effective means of sampling gravid and nulliparious female Ae. aegypti and Ae.…”

The State of the Art of Lethal Oviposition Trap-Based Mass Interventions for Arboviral Control

“…Encouraged by the potential impact of large-scale trapping, a series of interventions using mass deployments of large AGOs were conducted in Puerto Rico from 2011 to 2014 [56,57] to exploit the greater visual and olfactory attraction afforded by large AGOs (Figure 1e) in a mass-trapping intervention. The studies included two intervention zones, La Margarita (2011–2014) and Villodas (2013–2014), each with a paired control area.…”

“…The studies included two intervention zones, La Margarita (2011–2014) and Villodas (2013–2014), each with a paired control area. The experimental design for both studies involved placing three AGOs per house at 81% [56] and 85% [57] of the houses in each intervention area. The researchers implemented a two-month service interval to take advantage of the extended activity of the large AGOs (long-lasting stickiness of the glue panel and large water volume and closed design reduces likelihood the traps will dry) to limit the amount of staff and resources required to service the large number of traps deployed (>700 traps).…”

“…aegypti . The infusions can last for up to 2 months or longer (see Mackay [39] or Barrera [56]). This can be tasked to residents who can simply change water and add hay or other organic material provided by the control program.…”

“…Due to the limited data available, this is a “best guess” estimate based on the success of the AGO interventions (81%–85% coverage) in Puerto Rico [56,57], which resulted in a positive epidemiological outcome [58]. However, this value will likely depend on variables such as the type and size of the trap deployed and the density and type of housing in the intervention location.…”