Use of the fluorescence activated cell sorter to enrich dendritic cells from mouse spleen

Crowley, Mary T.; Inaba, Kayo; Witmer-Pack, Margit D.; Gezelter, Stuart; Steinman, Ralph M.

doi:10.1016/0022-1759(90)90318-p

Cited by 97 publications

(83 citation statements)

References 25 publications

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“…Initially we detected only low levels of CD4 on murine DC (11), as did Crowley et al (22). In what at that time appeared as a striking contrast, a proportion of human and rat DC stained strongly for surface CD4 (21,23,24).…”

Section: Endritic Cells (Dc) 3 Collect Transport and Then Presentsupporting

confidence: 53%

CD4 and CD8 Expression by Dendritic Cell Subtypes in Mouse Thymus and Spleen

Vremec

Pooley

Hochrein

et al. 2000

The Journal of Immunology

719

691

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The dendritic cells (DC) of mouse spleen and thymus were examined for expression of CD4 and CD8. Provided care was taken to avoid selective extraction or selective depletion of DC subpopulations, three main types of DC were detected in mouse spleen: a major new population of CD4+8− DEC-205low CD11bhigh DC, together with the previously described CD4−8− DEC-205low CD11bhigh DC and CD4−8αα+ DEC-205high CD11blow DC. The CD4 on the surface of the CD4+ splenic DC subpopulation was produced by the DC themselves, and CD4 RNA transcripts were present. Likewise, the CD8α on the surface of the splenic CD8+ DC was shown to be a product of the DC themselves, in agreement with earlier evidence. All three spleen DC types would be considered as mature, based on expression of CD80, CD86, and CD40 as well as on T cell stimulating function. Mouse thymuses appeared to contain two DC types; both were DEC-205highCD11blow, but they differed in the level of CD8αα expression. However, as well as this authenticated marker expression, immunofluorescent staining was also found to reflect a series of artifacts, due to the autofluorescence of contaminating cells and due to pickup of CD4 and CD8αβ. By constructing mice chimeric for the hemopoietic lineages using mixtures of wild-type bone marrow with CD4null or CD8αnull bone marrow, a marked pickup by thymic DC of Ags derived from thymocytes was demonstrated.

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Section: Endritic Cells (Dc) 3 Collect Transport and Then Presentsupporting

confidence: 53%

CD4 and CD8 Expression by Dendritic Cell Subtypes in Mouse Thymus and Spleen

Vremec

Pooley

Hochrein

et al. 2000

The Journal of Immunology

719

691

View full text Add to dashboard Cite

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“…Two-color staining was used to identify differences in marker expression between cells of the small and large subsets. CD11c was used as the main DC marker, because it is strongly expressed by DC and particularly by splenic DC (16,17). A majority of large LTC-DC express CD11c on their surface (70-80%) (Fig.…”

Section: Nonadherent Cells Produced In Ltc Represent Two Major Subsetsmentioning

confidence: 99%

Identification of progenitor cells in long-term spleen stromal cultures that produce immature dendritic cells

Wilson

O’Neill

2000

Proc. Natl. Acad. Sci. U.S.A.

101

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“…Instead, we and others used the distinct morphology of DC to identify, enrich and begin to characterize these cells in several tissues and species, and to prepare the first monoclonals, i.e. 33D1, now anti-DCIR2 [16][17][18] and N418 anti-CD11c [19,20]. The distinct morphology that we used to guide our preparation of DC has now been observed in living lymph nodes, where each DC continually probes its environment by extending and retracting processes [21].…”

Section: Introductionmentioning

confidence: 99%

Dendritic cells: Understanding immunogenicity

2007

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The impetus for the discovery of dendritic cells in 1972 was to understand immunogenicity, the capacity of an antigenic substance to provoke immunity. During experiments to characterize "accessory" cells that enhanced immunity, we spotted unusual stellate cells in mouse spleen. They had a distinct capacity to form and retract processes or dendrites and were named dendritic cells (DC). DC proved to be different from other cell types and to be peculiarly immunogenic when loaded with antigens. When Langerhans cells were studied, immunogenicity was found to involve two steps: antigen presentation by immature DC and maturation to elicit immunity. Antigenbearing DC were also immunogenic in vivo and were therefore termed "nature's adjuvants". Several labs then learned to generate large numbers of DC from progenitors, which accelerated DC research. Tolerogenicity via DC, including the control of foxp3 + suppressor T cells, was recently discovered. Two areas of current research that I find intriguing are to identify mechanisms for antigen uptake and processing, and for the control of different types of immunity and tolerance. These subjects should be studied in vivo with clinically relevant antigens, so that the activities of DC can be better integrated into the prevention and treatment of disease in patients.

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Use of the fluorescence activated cell sorter to enrich dendritic cells from mouse spleen

Cited by 97 publications

References 25 publications

CD4 and CD8 Expression by Dendritic Cell Subtypes in Mouse Thymus and Spleen

CD4 and CD8 Expression by Dendritic Cell Subtypes in Mouse Thymus and Spleen

Identification of progenitor cells in long-term spleen stromal cultures that produce immature dendritic cells

Dendritic cells: Understanding immunogenicity

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