Use of the Japanese Medaka ( Oryzias latipes ) and Guppy ( Poecilia reticulata ) in Carcinogenesis Testing Under National Toxicology Program Protocols

Hawkins, William E.; Walker, William W.; Fournie, John W.; Manning, C. Steve; Krol, Rena M.

doi:10.1080/01926230309771

Cited by 11 publications

(13 citation statements)

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“…Zebrafish was the first small fish that was used for carcinogenesis studies (Stanton, 1965); however, medaka was soon adopted for similar analyses (Ishikawa et al, 1975;Matsushima and Sugimura, 1976). By the 1980s, medaka had been used in a number of studies as a test system for spontaneous as well as induced carcinogenesis (Ishikawa et al, 1984;Masahito et al, 1989), an application that has come into vogue again (Boorman et al, 1997;Walter, 2001;Winn, 2001;Carlson et al, 2002;Hawkins et al, 2003;Kiparissis et al, 2003).…”

Section: Early Accomplishments Compiled By Yamamotomentioning

confidence: 97%

Medaka as a research organism: past, present and future

Shima

Mikami

2004

Mechanisms of Development

172

112

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Section: Early Accomplishments Compiled By Yamamotomentioning

confidence: 97%

Medaka as a research organism: past, present and future

Shima

Mikami

2004

Mechanisms of Development

172

112

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“…In comparison to many rodent species (Ward 1981), advantages of zebrafish include heightened sensitivity to carcinogens with a very low background tumor rate (Reddy et al 1999a). Due to their short generation time, studies can be conducted near the life span of the individuals in about 12 months (Hawkins et al 2003), an invaluable advantage in longterm studies that involve environmentally realistic exposures. The potential of zebrafish as a model for cancer research has been emphasized by the demonstrated similarities of transcriptome profiles between zebrafish carcinogen-induced tumor and human cancer (Lam et al 2006).…”

Section: Introductionmentioning

confidence: 99%

Chronic dietary exposure of zebrafish to PAH mixtures results in carcinogenic but not genotoxic effects

Larcher

Perrichon

Vignet

et al. 2014

Environ Sci Pollut Res

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Polycyclic aromatic hydrocarbons (PAHs) are ubiquitous contaminants that can be present at high levels as mixtures in polluted aquatic environments. Many PAHs are potent mutagens and several are well-known carcinogens. Despite numerous studies on individual compounds, little is known about the toxicity of PAHs mixtures that are encountered in environmental situations. In the present work, zebrafish were continuously fed from 5 days post-fertilisation to 14 months post-fertilisation (mpf) with a diet spiked with fractions of either pyrolytic (PY), petrogenic light oil (LO), or petrogenic heavy oil (HO) origin at three concentrations. A decrease in survival was identified after 3 mpf in fish fed with the highest concentration of HO or LO, but not for PY. All PAH fractions caused preneoplastic and neoplastic disorders in long-term-exposed animals. Target tissues were almost exclusively of epithelial origin, with the bile duct epithelium being the most susceptible to chronic exposure to all PAH fractions, and with germ cells being the second most responsive cells. Significantly higher incidences of neoplasms were observed with increasing PAH concentration and exposure duration. The most severe carcinogenic effects were induced by dietary exposure to HO compared to exposure to LO or PY (45, 30 and 7 %, respectively, after 9 to 10 months of exposure to an intermediate concentration of PAHs). In contrast, earliest carcinogenic effects were detected as soon as 3 mpf after exposure to LO, including the lowest concentration, or to PY. PAH bioactivation and genotoxicity in blood was assessed by ethoxyresorufin-O-deethylase activity quantification and comet and micronuclei assays, respectively, but none of these were positive. Chronic dietary exposure of zebrafish to PAH mixtures results in carcinogenotoxic events that impair survival and physiology of exposed fish.

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“…Over the past decade, small fish species have been proven useful both as environmental sentinels and as versatile animals in toxicity and carcinogenicity bioassays [13][14][15]. The use of small fish models in cancer research has the obvious advantages of economy, rapid response, low background incidence of tumours and the opportunity to work large numbers of specimens when compared with the more traditional rodent models [16].…”

Section: Introductionmentioning

confidence: 99%

Simultaneous determination of N7-alkylguanines in DNA by isotope-dilution LC-tandem MS coupled with automated solid-phase extraction and its application to a small fish model

Chao

Wang

Yen

et al. 2007

Biochemical Journal

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In the present study, we report the development of a sensitive and selective assay based on LC (liquid chromatography)-MS/MS (tandem MS) to simultaneously measure N7-MeG (N7-methylguanine) and N7-EtG (N7-ethylguanine) in DNA hydrolysates. With the use of isotope internal standards (15N5-N7-MeG and 15N5-N7-EtG) and on-line SPE (solid-phase extraction), the detection limit of this method was estimated as 0.42 fmol and 0.17 fmol for N7-MeG and N7-EtG respectively. The high sensitivity achieved here makes this method applicable to small experimental animals. This method was applied to measure N7-alkylguanines in liver DNA from mosquito fish (Gambusia affinis) that were exposed to NDMA (N-nitrosodimethylamine) and NDEA (N-nitrosodiethylamine) alone or their combination over a wide range of concentrations (1-100 mg/l). Results showed that the background level of N7-MeG in liver of control fish was 7.89+/-1.38 mmol/mol of guanine, while N7-EtG was detectable in most of the control fish with a range of 0.05-0.19 mmol/mol of guanine. N7-MeG and N7-EtG were significantly induced by NDMA and NDEA respectively, at a concentration as low as 1 mg/l and increased in a dose-dependent manner. Taken together, this LC-MS/MS assay provides the sensitivity and high throughput required to evaluate the extent of alkylated DNA lesions in small animal models of cancer induced by alkylating agents.

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Use of the Japanese Medaka ( Oryzias latipes ) and Guppy ( Poecilia reticulata ) in Carcinogenesis Testing Under National Toxicology Program Protocols

Cited by 11 publications

References 0 publications

Medaka as a research organism: past, present and future

Medaka as a research organism: past, present and future

Chronic dietary exposure of zebrafish to PAH mixtures results in carcinogenic but not genotoxic effects

Simultaneous determination of N7-alkylguanines in DNA by isotope-dilution LC-tandem MS coupled with automated solid-phase extraction and its application to a small fish model

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