Use of the &lt;i&gt;psbA-trnH&lt;/i&gt; Region to Authenticate Medicinal Species of Fabaceae

Gao, Ting; Ma, Xianlei; Zhu, Xinghua

doi:10.1248/bpb.b13-00611

Cited by 12 publications

(12 citation statements)

References 23 publications

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“…Many researchers have reported that the intra-specific variation of psbA-trnH region is very low while the inter-specific divergence is very large in a wide variety of plants (Gao et al 2013;Ma et al 2010;Yang et al 2011;Yao et al 2009). Similar results were found in our study.…”

Section: Discussionsupporting

confidence: 92%

“…However, their identification based on morphological characteristics, which are similar, is extremely difficult. In fact, the psbA-trnH region has been used as a DNA barcode to authenticate various plants with similar morphological traits (Chen et al 2010;Gao et al 2013;Ma et al 2010;Yang et al 2011;Yao et al 2009). In the present work, we used for the first time the chloroplast psbA-trnH region for barcoding Physalis species.…”

Section: Discussionmentioning

confidence: 99%

“…cpDNA sequences (atpF-atpH, psbA-trnH, matK, rbcL) have been widely used as a tool in studies on plant phylogenetics and species identification. In particular, the psbA-trnH intergenic region has been found to be ubiquitous in plants; it has thus emerged as one of the popular DNA barcodes (Cbol Plant Working Group 2009;Gao et al 2013;Kress and Erickson 2007;Kress et al 2005;Yao et al 2009). However, few studies have reported the use of the psbA-trnH intergenic region in the barcoding of Physalis species.…”

Section: Because Of Their Highly Similar Morphological Characteristicmentioning

confidence: 99%

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Molecular identification of species of Physalis (Solanaceae) using a candidate DNA barcode: the chloroplast psbA–trnH intergenic region

Feng

Jiao

Zhu

et al. 2018

Genome

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Physalis L., an important genus of the family Solanaceae, includes many commercially important edible and medicinal species. Traditionally, species identification is based on morphological traits; however, the highly similar morphological traits among species of Physalis make this approach difficult. In this study, we evaluated the feasibility of using a popular DNA barcode, the chloroplast psbA-trnH intergenic region, in the identification of species of Physalis. Thirty-six psbA-trnH regions of species of Physalis and of the closely related plant Nicandra physalodes were analyzed. The success rates of PCR amplification and sequencing of the psbA-trnH region were 100%. MEGA V6.0 was utilized to align the psbA-trnH sequences and to compute genetic distances. The results show an apparent barcoding gap between intra- and interspecific variations. Results of both BLAST1 and nearest-distance methods prove that the psbA-trnH regions can be used to identify all species examined in the present study. In addition, phylogenetic analysis using psbA-trnH data revealed a distinct boundary between species. It also confirmed the relationship between species of Physalis and closely related species, as established by previous studies. In conclusion, the psbA-trnH intergenic region can be used as an efficient DNA barcode for the identification of species of Physalis.

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Section: Discussionsupporting

confidence: 92%

Section: Discussionmentioning

confidence: 99%

Section: Because Of Their Highly Similar Morphological Characteristicmentioning

confidence: 99%

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Molecular identification of species of Physalis (Solanaceae) using a candidate DNA barcode: the chloroplast psbA–trnH intergenic region

Feng

Jiao

Zhu

et al. 2018

Genome

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“…ITS2 region was used to identified dicotyledons, monocotyledons, gymnosperms and ferns, the success rates was different [ 13 ]. Fabaceae is a huge family, among them 91.3% of 104 Fabaceae medicinal species was identified successfully by using of trn H -psb A sequences [ 14 ]. Data from trn H -psb A region were analysed to illuminate molecular evolution of Maghrebian Medicago species and reveal high interspecific diversity and low intraspecific variation [ 15 ].…”

Section: Introductionmentioning

confidence: 99%

Potential DNA barcodes for Melilotus species based on five single loci and their combinations

Meng³

et al. 2017

PLoS ONE

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Melilotus, an annual or biennial herb, belongs to the tribe Trifolieae (Leguminosae) and consists of 19 species. As an important green manure crop, diverse Melilotus species have different values as feed and medicine. To identify different Melilotus species, we examined the efficiency of five candidate regions as barcodes, including the internal transcribed spacer (ITS) and two chloroplast loci, rbcL and matK, and two non-coding loci, trnH-psbA and trnL-F. In total, 198 individuals from 98 accessions representing 18 Melilotus species were sequenced for these five potential barcodes. Based on inter-specific divergence, we analysed sequences and confirmed that each candidate barcode was able to identify some of the 18 species. The resolution of a single barcode and its combinations ranged from 33.33% to 88.89%. Analysis of pairwise distances showed that matK+rbcL+trnL-F+trnH-psbA+ITS (MRTPI) had the greatest value and rbcL the least. Barcode gap values and similarity value analyses confirmed these trends. The results indicated that an ITS region, successfully identifying 13 of 18 species, was the most appropriate single barcode and that the combination of all five potential barcodes identified 16 of the 18 species. We conclude that MRTPI is the most effective tool for Melilotus species identification. Taking full advantage of the barcode system, a clear taxonomic relationship can be applied to identify Melilotus species and enhance their practical production.

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“…15) DNA barcoding has since been successfully applied towards the traceability of many herb medicines. [16][17][18][19][20][21][22] Additional candidate DNA barcoding genes in seed plants, which are used for identification of Chinese medicines and to confirm the identities of the original plants, are currently providing a basis for identifying Phellodendri Cortex.…”

mentioning

confidence: 99%

Comparative Analysis of DNA Barcoding and HPLC Fingerprint to Trace Species of Phellodendri Cortex, an Important Traditional Chinese Medicine from Multiple Sources

Zhang

et al. 2016

Biological & Pharmaceutical Bulletin

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Phellodendri Cortex is derived from the dried barks of Phellodendron genus species, has been extensively used in traditional Chinese medicine. The cortex is divided into two odorless crude drugs Guanhuangbo and Huangbo. Historically, it has been difficult to distinguish their identities due to a lack of identification methods. This study was executed to confirm the identity and to ensure the species traceability of Phellodendri Cortex. In the current study, analysis is based on the internal transcribed spacer (ITS) and psbA-trnH intergenic spacer (psbA-trnH) barcodes and HPLC fingerprint was carried out to guarantee the species traceability of Guanhuangbo and Huangbo. DNA barcoding data successfully identified the three plants of the Phellodendron genus species by ITS psbA-trnH, with the ability to distinguish the species origin of Huangbo. Moreover, the psbA-trnH data distinguished Guanhuangbo and Huangbo except to trace species. The HPLC fingerprint data showed that Guanhuangbo was clearly different from Huangbo, but there was no difference between the two origins of Huangbo. Additionally, the result of hierarchical clustering analysis, based on chlorogenic acid, phellodendrine, magnoflorine, jatrorrhizine, palmatine and berberine, was consistent with the HPLC fingerprint analysis. These results show that DNA barcoding and HPLC fingerprint can discriminate Guanhuangbo and Huangbo. However, DNA barcoding is more powerful than HPLC fingerprint for species traceability in the identification of related species that are genetically similar. DNA barcoding is a useful scientific tool to accurately confirm the identities of medicinal materials from multiple sources. Key words Phellodendri Cortex; DNA barcoding; HPLC fingerprint; species traceabilityPhellodendri Cortex is derived from the dried barks of Phellodendron amurense RUPR., Phellodendron chinense SCHNEID. and the variant Phellodendron chinense var. glabriusculum SCHNEID. (Rutaceae).1) It is commonly used in traditional oriental medicine, and it has demonstrated a wide range of biological and pharmacological activities-antimicrobial, anti-inflammatory and anticancer-in numerous studies.2,3) In the Chinese Pharmacopoeia (2000 version), P. amurense and P. chinense are listed as the original plants of Phellodendri Cortex.4) However, P. amurense is listed as an endangered species and is strictly protected by law because this resource has decreased dramatically. 5) Additionally, there are differences between P. amurense and P. chinense regarding plant morphology and chemical composition. Thus, to control the quality of the herb and to protect the wild resource of P. amurense, Phellodendri Cortex is officially listed in the Chinese Pharmacopoeia (2005 version) as two different crude drugs, Guanhuangbo and Huangbo. 6) Guanhuangbo is derived from the P. amurense bark, while Huangbo is derived from the P. chinense bark. Since the wild resource of P. chinense is insufficient, the cultivated herb of P. chinense var. glabriusculum is also utilized in the production of crud...

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Use of the <i>psbA-trnH</i> Region to Authenticate Medicinal Species of Fabaceae

Cited by 12 publications

References 23 publications

Molecular identification of species of Physalis (Solanaceae) using a candidate DNA barcode: the chloroplast psbA–trnH intergenic region

Molecular identification of species of Physalis (Solanaceae) using a candidate DNA barcode: the chloroplast psbA–trnH intergenic region

Potential DNA barcodes for Melilotus species based on five single loci and their combinations

Comparative Analysis of DNA Barcoding and HPLC Fingerprint to Trace Species of Phellodendri Cortex, an Important Traditional Chinese Medicine from Multiple Sources

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