Use of the RAPD Technique for Identification of<i>Fusarium oxysporum</i>f. sp.<i>dianthi</i>from Carnation

Manulis, Shulamit; Kogan, N.; Reuven, M.; Ben‐Yephet, Y.

doi:10.1094/phyto-84-98

Cited by 84 publications

(42 citation statements)

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“…Many studies have shown that molecular markers could detect genetic variation within populations to a certain extent, including pathogenicity variation, geographical differences and host differences (Goodwin and Annis, 1991;Grajal-Martin et al, 1993;Manulis et al, 1994;Nyasse et al, 1999). In the present study, considerable genetic diversity of P. capsici was observed with ISSR markers, based on 51 isolates collected from Anhui Province.…”

Section: Discussionmentioning

confidence: 50%

Genetic diversity of Phytophthora capsici (Pythiaceae) isolates in Anhui Province of China based on ISSR-PCR markers

Li¹,

Cao²,

Dai³

et al. 2012

Genet. Mol. Res.

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ABSTRACT. Phytophthora capsici is a plant pathogenic oomycete that damages numerous crops worldwide. Consequently, interest in research on the genetic structure of this species has grown in recent decades. However, there is little information about P. capsici in eastern China. We investigated the genetic diversity of P. capsici isolates from three large regions of Anhui Province in eastern China based on ISSR-PCR technology. Thirteen random primers were screened and used to amplify DNA from 51 samples. We obtained 158 reproducible ISSR fragments, of which 90% were polymorphic, revealing a high degree of polymorphism among the isolates. Genetic similarity coefficients among all the isolates ranged from 0.56 to 0.94, with a mean of 0.84 based on the ISSR data, indicating a high level of genetic variation in these P. capsici isolates. Cluster analysis using UPGMA indicated that the Anhui isolates were divided into seven groups according to the DNA fingerprints, although there was no correlation between the ISSR group and geographic origin. Isolates from the same location showed no clustering based on the year of sampling. AMOVA partitioned variability among (13.6%) and within populations (86.4%). The gene flow among populations ranged from 2.804 to 4.937, with a mean of 3.545, indicating highly frequent gene exchange. Genetic distances and genetic differentiation were negatively correlated with geographic distances. These results lead us to suggest that this pathogen has considerable evolutionary potential, which will enable it to adapt to and overcome management strategies over time.

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Section: Discussionmentioning

confidence: 50%

Genetic diversity of Phytophthora capsici (Pythiaceae) isolates in Anhui Province of China based on ISSR-PCR markers

Li¹,

Cao²,

Dai³

et al. 2012

Genet. Mol. Res.

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“…(Crowhurst et al, 1991). Recently, RAPDs have been shown to be able to differentiate between pathogenic and non-pathogenic F. oxysporum isolated from carnation plants in Israel, and possibly to distinguish Fod race 2 from race 4 (Manulis et al, 1994).…”

Section: Fusarium Oxysporum Schlecht Causes Vascular Wiltmentioning

confidence: 99%

“…Recently, Manulis et al (1994) carried out a similar study examining the genetic diversity off oxysporum isolated from carnation plants in Israel. They demonstrated that RAPD analysis could distinguish between pathogenic and non-pathogenic isolates, and in the majority of cases between isolates of race 2 and one isolate of race 4.…”

Section: Random Amplified Polymorphic Dna (Rapd ) Analysismentioning

confidence: 99%

Characterisation ofFusarium oxysporum isolated from carnation in Australia based on pathogenicity, vegetative compatibility and random amplified polymorphic DNA (RAPD) assay

et al. 1996

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Isolates of F. oxysporum collected from symptomless carnation cuttings from Australian carnation growers properties, together with isolates from national collections, were screened for pathogenicity and grouped according to vegetative compatibility and random amplified polymorphic DNA (RAPD) patterns. The collection of 82 Australian isolates sorted into 23 different vegetative compatibility groups (VCGs). Of 69 isolates tested for pathogenicity, 24 were pathogenic to carnations, while the remaining 45 were non-pathogenic. All pathogenic isolates were within two VCGs, one of which was also compatible with an isolate obtained from an international culture collection, and which is known to represent VCG 0021 and race 2. Race status of the two pathogenic VCGs remains unknown. The RAPD assay revealed distinct DNA banding patterns which could distinguish pathogenic from non-pathogenic isolates as well as differentiate between isolates from the two pathogenic VCGs. Fusarium oxysporumSchlecht. causes vascular wilt in a broad range of host plants including carnations (Dianthus caryophyllus L.). This species of Fusarium

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“…The ability of Trichoderma to control R. solani varies considerably, and it is possible to improve its biological control efficiency by the selection of isolates with high antagonistic potential and adapted to certain ecological or geographical areas (Noronha et al 1996). The Random Amplified Polymorphic DNA (RAPD) procedure developed by Williams et al (1990) and Welsh & Mc Clelland (1990) that involves simultaneous amplification of several anonymous loci in the genome using primers of arbitrary sequence has been used for genetic, taxonomic and ecological studies of several fungi (Crowhurst et al, 1995;Fungaro et al ,1996;Manulis et al, 1993;Zinno et al, 1998;Abbasi, et al, 1999;Paavanem-Huhtala et al, 2000). Fourteen strains of Trichoderma were studied by Santos (1992), using molecular markers of RAPD among other techniques, and it was possible to verify the natural genetic variability and to divide the strains in similarity groups, as well as differentiating the original strains of different regions.…”

Section: Introductionmentioning

confidence: 99%

Randomly Amplified Polymorphic DNA of Trichoderma isolates and antagonism against Rhizoctonia solani

Góes

Costa

Freire

et al. 2002

Braz. arch. biol. technol.

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Use of the RAPD Technique for Identification ofFusarium oxysporumf. sp.dianthifrom Carnation

Cited by 84 publications

References 0 publications

Genetic diversity of Phytophthora capsici (Pythiaceae) isolates in Anhui Province of China based on ISSR-PCR markers

Genetic diversity of Phytophthora capsici (Pythiaceae) isolates in Anhui Province of China based on ISSR-PCR markers

Characterisation ofFusarium oxysporum isolated from carnation in Australia based on pathogenicity, vegetative compatibility and random amplified polymorphic DNA (RAPD) assay

Randomly Amplified Polymorphic DNA of Trichoderma isolates and antagonism against Rhizoctonia solani

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