Use of the Ussing chamber technique to study nutrient transport by epithelial tissues

He, Liuqin; Ye, Yin; Li, Tiejun; Huang, Ru‐Lin; Xie, Mingyong; Wu, Zhenlong; Wu, Guoyao

doi:10.2741/4178

Cited by 24 publications

(3 citation statements)

References 57 publications

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“…Powerful methodologies include measurements of mRNA and protein (total and phosphorylated) abundances in cells and tissues using quantitative real-time PCR and western blotting techniques. Additionally, as for other nutrients (244,245), transport of 3 H 2 O and ions by cells or tissues in the Ussing chamber system provides a functional indication of AQP abundance and/or activity (Figure 4). Future investigations of AQP in the mammalian female reproductive system require specific AQP blockers/inhibitors or animal models with defined phenotypes for AQP deficiencies.…”

Section: Discussionmentioning

confidence: 99%

Aquaporins in the female reproductive system of mammals

et al. 2015

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Water and ion accumulation is the driving force for rapid expansion of the amnion and allantois of mammalian placentae during early gestation, and, therefore, essential for embryonic/fetal growth and survival. Aquaporins (AQP) are a family of small integral plasma membrane proteins that primarily transport water across the plasma membrane. To date, thirteen AQP isoforms (AQP 0-12) have been identified in mammals. AQP 1, 2, 3, 4, 5, 6, 7, 8, 9, and 11 are expressed in the female reproductive tract. Based on their structural and functional properties, AQPs are divided into three subgroups: classical aquaporins (AQP 0, 1, 2, 4, 5, 6, and 8), aquaglyceroporins (AQP 3, 7, 9, and 10), and superaquaporins (AQP 11 and 12). Expression of AQPs in the uterus and placenta is regulated by hormones and nutrients to maintain fluid homeostasis in the conceptus. The underlying mechanisms may involve signal transduction pathways mediated by cAMP, MAPK, PKC, and PI3K/Akt/mTOR. Such new knowledge will advance basic understanding of mammalian reproductive biology to enhance embryonic/fetal survival, growth and development in women and livestock.

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Section: Discussionmentioning

confidence: 99%

Aquaporins in the female reproductive system of mammals

et al. 2015

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“…The in vivo lysine transport was tested via Ussing chamber according to our previous report [20]. Briefly, 10-cm segments were excised from the proximal jejunum and immediately placed in oxygenated HEPES-Tris buffer solution (pH 7.4) at 37 o C. Mucosa sample were made for 4 replicates respectively, aperture area was set for 0.5cm 2 as the exposed tissue surface area, and the fluid volume in each chamber was 5 ml.…”

Section: Methodsmentioning

confidence: 99%

Lysine Restriction Affects Feed Intake and Amino Acid Metabolism via Gut Microbiome in Piglets

Yin

Han

et al. 2017

Cell Physiol Biochem

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Background/Aims: Our previous reports suggested that dietary supplementation with lysine influenced intestinal absorption and metabolism of amino acids. In this study, we further investigated the effect of lysine restriction (30%) on feed intake and we also tested the hypothesis that gut microbiome contributed to the potential mechanism of lysine restriction-mediated feeding behavior. Here, we profiled gut microbial communities by sequencing 16S ribosomal ribonucleic acid (rRNA) genes from gut samples as well as growth performance, serum hormones, and intestinal lysine transport in a piglet model. Results: Piglets preferred to the lysine restricted diet when giving three diets and the feed intake was markedly higher in the lysine-restricted group than that in the control group. Altered hormones (leptin, CCK, and ghrelin) might contribute to the feeding behavior caused by lysine restriction. Meanwhile, lysine transporting ability (SLC7A1 and SLC7A2 expression, intestinal electrophysiological changes, and amino acid pool in mesenteric vein) was decreased in response to lysine restriction. Through deep sequencing of bacterial rRNA markers, we observed that bacterial diversity was enhanced in the lysine-restricted group (Shannon H, PD, and Chao1). At the phylum level, lysine restriction enhanced gut Actinobacteria, Saccharibacteria, and Synergistetes abundances. At the family level, Moraxellaceae, Halomonadaceae, Shewanellaceae, Corynebacteriaceae, Bacillaceae, Comamonadaceae, Microbacteriaceae, Caulobacteraceae, and Synergistaceae abundances were increased in response to lysine restriction. Predictive functional profiling of microbial communities by PICRUSt also confirmed that dietary lysine restriction affected gut microbiome, which might further mediate amino acid metabolism, membrane transport, and endocrine system. Conclusion: Our results indicated that lysine restriction inhibited intestinal lysine transport and promoted feed intake, which might be associated with gut microbiome.

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“…Prostaglandin E2 (PGE2) and interleukin-1beta (IL-1β) are the substances which are elevated in large intestine diseases and participate in the development of pathologies (Kamiyama et al, 2006;Sheng et al, 2006;Al-Sadi et al, 2012;Carmi et al, 2013;Voronov et al, 2013;Safdari et al, 2016;Zielinska et al, 2018). However, their effect on the barrier properties of different parts of the colon has not been studied yet.…”

Section: Introductionmentioning

confidence: 99%

Heterogeneity of the barrier properties of the colon in rat

Bekusova

Fatyykhov

Amasheh

et al. 2021

BioComm

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The incidence of colorectal cancer in different parts of the large intestine is not the same, as tumors more often appear in the distal part of the colon compared to the proximal one. The purpose of this study was to investigate heterogeneity of the barrier properties of the colon and clarify the effects of Prostaglandin E2 and interleukin-1beta on its different parts. An in-depth analysis of short circuit current, transepithelial electrical resistance and paracellular permeability for sodium fluorescein in Ussing chambers showed that the proximal part of the colon was less permeable compared to the distal one and the substances had different effects on the parameters of permeability in different parts of the colon. We suppose that heterogeneity of the barrier properties of the colon and various effects of their regulation by local molecular agents may determine different incidence of pathologies in the colon.

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Use of the Ussing chamber technique to study nutrient transport by epithelial tissues

Cited by 24 publications

References 57 publications

Aquaporins in the female reproductive system of mammals

Aquaporins in the female reproductive system of mammals

Lysine Restriction Affects Feed Intake and Amino Acid Metabolism via Gut Microbiome in Piglets

Heterogeneity of the barrier properties of the colon in rat

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