Use of triphenyl tetrazolium in Motility Test Medium

Kelly, Ann T.; Fulton, MacDonald

doi:10.1093/ajcp/23.5_ts.512

Cited by 22 publications

(14 citation statements)

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“…Each antigen was tested in the viable as well as heat-killed state (100 C, 1 hr); incubation was at 37 C for 18 hr. All challenge suspensions also were inoculated into a semisolid motility test medium containing 0.005% triphenyl tetrazolium chloride (Kelly and Fulton, 1953) and reactions noted after 18 hr at 37 C. Mortality in inoculated mice was recorded for 28 days and the LD50 calculated by the method of Reed and Muench (1938).…”

Section: Methodsmentioning

confidence: 99%

H ANTIGEN OFSALMONELLA TYPHOSA

Tully

Gaines

1961

J Bacteriol

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Section: Methodsmentioning

confidence: 99%

H ANTIGEN OFSALMONELLA TYPHOSA

Tully

Gaines

1961

J Bacteriol

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“…6). Motility was demonstrated in semisolid medium using triphenyl tetrazolium [43]. The colonies of the strains were white-yellowish, convex and circular with a mean diameter of 4.6 mm and regular margins after 48 hrs.…”

Section: Resultsmentioning

confidence: 99%

“…The Schaeffer and Fulton [42] method was used to determine whether the strains were spore-forming. Strain motility was demonstrated using triphenyl tetrazolium [43] in semisolid medium (per liter: 3.0 g beef extract, 10.0 g pancreatic digest of casein, 5.0 g NaCl, 4.0 g agar).…”

Section: Methodsmentioning

confidence: 99%

Diversity of bacteria associated with corn roots inoculated with Canadian woodland soils, and description of Pseudomonas aylmerense sp. nov.

Tchagang

Overy

et al. 2018

Heliyon

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Bacteria associated with corn roots inoculated with soils collected from the Canadian woodlands were isolated and characterized. Genus-level identification based on 16S rRNA sequence analysis classified the 161 isolates in 19 genera. The majority (64%) of the isolates were affiliated with the genus Pseudomonas. Further analysis of the Pseudomonas isolates based on BLASTn and rpoD-rpoB-gyrB concatenated gene phylogeny revealed three unique clusters that could not be assigned to known species. This study reports the taxonomic description of one of the distinct lineages represented by two strains (S1E40T and S1E44) with P. lurida LMG 21995T, P. costantinii LMG 22119T, P. palleroniana LMG 23076T, P. simiae CCUG 50988T and P. extremorientalis LMG 19695T as the closest taxa. Both strains showed low ANIm (<90%) and genome-based DNA-DNA hybridization (<50%) values, which unequivocally delineated the new strains from the closest relatives. These findings were supported by multilocus sequence analysis (MLSA) and DNA fingerprinting. In addition, growth characteristics and biochemical tests revealed patterns that differed from the related species. Strains S1E40T and S1E44 are Gram-negative, aerobic, rod-shaped and motile by at least one flagellum; and grew optimally at 30 °C. The predominant polar lipid is phosphatidylethanolamine while the major respiratory quinone is ubiquinone-9. Based on phenotypic and genotypic data presented here, strains S1E40T and S1E44 represent a novel species for which the name Pseudomonas aylmerense sp. nov. is proposed. The type strain is S1E40T (= LMG 30784T = DOAB 703T = HAMI 3696T) with a G + C content of 61.6%.

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“…Growth occurred both in aerobic and anaerobic conditions but profuse growth was seen under anaerobic condition. Growth did not occur at 15 0 C and 25 0 C. But growth was observed at 35 0 C. The bacterium did not show growth above 45 0 C. The bacterium did not form spores 54 . The biochemical studies (Table 4) of the bacterium showed no liquefaction of gelatin, citrate was utilized as a source of carbon, while urea was un-affected 55 .…”

Section: Lactobacillus Acidophilusmentioning

confidence: 91%

“…It had a mean diameter of 1.05 um. The bacterium was non motile 54 . Growth occurred both in aerobic and anaerobic conditions but profuse growth was seen under anaerobic condition.…”

Section: Lactobacillus Acidophilusmentioning

confidence: 99%

Isolation and Freeze Drying of Certain Probiotic Strains

Khan¹,

Jaffery²

2013

Int. Res. J. Pharm.

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The aim of the present study was to evaluate few probiotics strains available commercially in Lahore city and isolation and identification of strains from these commercial probiotics. The identified and isolated probiotic strains were freeze dried. These strains included Lactobacillus acidophilus, Bifidumbacterium bifidus and Saccharomyces boulradii. Both the commercial and laboratory freeze dried probiotic micro-organisms were subjected to microbial studies. The isolation of the microbes was done by using different types of growth media and identification of microbes was based on fermentation of different carbohydrates, gas production from glucose, growth at various temperature and tolerance to sodium chloride, colony characteristics and staining. The actual microbial count in laboratory of commercial probiotics indicated that there was a discrepancy in the viable count stated on the label of Lactobacillus acidophilus, Bifidumbacterium bifidus i.e., the actual viable count is less than the stated viable count on the label. This laboratory evidence suggest that bacteria cannot survive longer time on shelves which is probably the main reason that probiotics inspite of their in vivo effectiveness have not found a good way in physician prescription pattern. However, such discrepancy was not observed in case of yeast. The microbiological studies suggested that it is feasible to isolate, identify and freeze dry the microbes in the laboratory and pilot plant scale. The microbial count after freeze drying of laboratory prepared probiotics had shown that anaerobic bacteria (Bifidumbacterium bifidus) survived more during the freeze drying as compared to Lactobacillus acidophilus and yeast survive more than the both bacterial strains. Cite this article as:Fasiha shah, Kahlid Idrees Khan and Kausar Tasneem Jaffery. Isolation and freeze drying of certain probiotic strains. Int. Res. J. Pharm. 2013; 4(11):40-45 http://dx.

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Use of triphenyl tetrazolium in Motility Test Medium

Cited by 22 publications

References 0 publications

H ANTIGEN OFSALMONELLA TYPHOSA

H ANTIGEN OFSALMONELLA TYPHOSA

Diversity of bacteria associated with corn roots inoculated with Canadian woodland soils, and description of Pseudomonas aylmerense sp. nov.

Isolation and Freeze Drying of Certain Probiotic Strains

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