Use of two formulations of Colilert and QuantiTray™ for assessment of the bacteriological quality of water

Fricker, E.J.; Illingworth, K.S.; Fricker, C. R.

doi:10.1016/s0043-1354(96)00342-9

Cited by 57 publications

(42 citation statements)

References 7 publications

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“…Similar results were obtained by other authors studying natural and drinking waters (Fricker et al 1997;Eckner 1998;Schets et al 2002;Ribas et al 2005), or wastewater samples ( as false-positive coliforms (Davies et al 1995;Landre et al 1998). In addition, b-galactosidase has been found to be present in non-coliform strains, which makes the level of enzymatic activity a result of the proportion of target and non-target bacteria in rapid enzymatic assays without a cultivation step (Tryland & Fiksdal 1998 (Fricker et al 1997;Eckner 1998;Yakub et al 2002;Buckalew et al 2006). However, since the Colilert w system requires short incubation periods and is easy to apply, it can be useful for enumerating coliforms from natural waters and wastewaters (Grasso et al 2000;Kramer & Liu 2002;Eccles et al 2004).…”

Section: Discussionsupporting

confidence: 89%

Are the defined substrate-based methods adequate to determine the microbiological quality of natural recreational waters?

Valente

Pedro

Alonso

et al. 2009

Journal of Water and Health

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Monitoring the microbiological quality of water used for recreational activities is very important to human public health. Although the sanitary quality of recreational marine waters could be evaluated by standard methods, they are time-consuming and need confirmation. For these reasons, faster and more sensitive methods, such as the defined substrate-based technology, have been developed. In the present work, we have compared the standard method of membrane filtration using Tergitol-TTC agar for total coliforms and Escherichia coli, and Slanetz and Bartley agar for enterococci, and the IDEXX defined substrate technology for these faecal pollution indicators to determine the microbiological quality of natural recreational waters.

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Section: Discussionsupporting

confidence: 89%

Are the defined substrate-based methods adequate to determine the microbiological quality of natural recreational waters?

Valente

Pedro

Alonso

et al. 2009

Journal of Water and Health

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“…Water samples (10 mL) were diluted 10-fold with sterile distilled water and were subjected to most probable number analysis using a commercial test kit (Colilert 18/QuantiTray TM , IDEXX Laboratories, Tokyo, Japan) (Fricker et al 1997). The samples were incubated at 37°C for 18 h, in accordance with the manufacturer's instructions.…”

Section: Escherichia Colimentioning

confidence: 99%

Anthropogenic impacts on water quality of the lagoonal coast of Fongafale Islet, Funafuti Atoll, Tuvalu

et al. 2013

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Water pollution, evident by negative values of redox potential in waters, occurs at the lagoonal coast located near the densely populated area of Fongafale Islet on Funafuti Atoll, Tuvalu, Central Pacific. Sediment microbial quinone analysis revealed that these coastal sediments exhibit 2.7-10.4 times more microbial biomass, significantly different microbial community structure and low microbial diversity, when compared to an undisturbed natural coastal sediment. Thus, the pollution is chronic. By considering the total land use/coverage on the islet, the situation of septic tank installations, temporal changes in water redox potential and Escherichia coli numbers in the coastal waters and the spatial distribution of acid volatile sulfide in the sediments, we conclude that domestic wastewater is the primary source of pollution. This pollution is proposed to occur via the following mechanism: during ebb tides, domestic wastewater leaking from bottomless septic tanks and pit toilets run off into the lagoonal coast. Tide changes control the pollution load of domestic wastewater.

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“…While this test is not completely specific for E. coli (Rice et al 1991;Fricker & Fricker 1996a) other studies (Fricker, unpublished) have suggested that this test is a more useful confirmatory test than production of indole from tryptophan at 448C. Furthermore, these studies have shown that in excess of 99.5% of strains of E. coli recovered Numerous studies have demonstrated that confirmation of positive results is not required (Cowburn et al 1994;Fricker et al 1997;Niemela et al 2003). However, in this study we chose to confirm the identity of all presumptive positive results using tests for cytochrome oxidase, production of b-D-glucuronidase and production of indole from tryptophan at 448C.…”

Section: Introductionmentioning

confidence: 99%

Use of the ISO 9308-1 procedure for the detection of E. coli in water utilizing two incubation temperatures and two confirmation procedures and comparison with defined substrate technology

Fricker¹,

Bullock

Murrin

et al. 2008

Journal of Water and Health

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Disinfected and non-disinfected samples have been used to determine the accuracy of the ISO procedure (ISO 9308-1) for detection of E. coli in drinking water. Samples were analysed using the ISO procedure at both 36 and 448C and using the defined substrate technology method -18). Utilizing the confirmation procedure described in ISO 9308-1, large numbers of false positive E. coli results were obtained using the ISO primary isolation procedure at 368C. However, when glucuronidase production was used as the confirmation procedure, the 'confirmed' count of E. coli was lowest with ISO 9308-1 performed at 368C. When TTC medium was incubated at 368C confirmation using production of indole at 448C resulted in 29% more 'E. coli' being recovered than when confirmation was performed using production of glucuronidase. When 448C was used as the primary isolation temperature the difference between the number of 'confirmed' E. coli identified using the two confirmation procedures was less than 1% and was not significant. Identification of isolates which 'confirmed' when using production of indole at 448C as the test method but 8which failed to produce b-D-glucuronidase, showed that the majority of these isolates were Klebsiella oxytoca.

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Use of two formulations of Colilert and QuantiTray™ for assessment of the bacteriological quality of water

Cited by 57 publications

References 7 publications

Are the defined substrate-based methods adequate to determine the microbiological quality of natural recreational waters?

Are the defined substrate-based methods adequate to determine the microbiological quality of natural recreational waters?

Anthropogenic impacts on water quality of the lagoonal coast of Fongafale Islet, Funafuti Atoll, Tuvalu

Use of the ISO 9308-1 procedure for the detection of E. coli in water utilizing two incubation temperatures and two confirmation procedures and comparison with defined substrate technology

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