Use of two replacements of serum during bovine embryo culture in vitro

Abstract

This study evaluated the effect of two commercial serum replacements (Ultroser G 1 and CPSR-3 1 ) on in vitro bovine embryo culture. In Experiment 1, zygotes were cultured in SOF Ultroser G 1 (2, 4 and 6%), SOF CPSR-3 1 (2, 4 and 6%), and SOF 5% FCS (control). Blastocyst rates obtained after culturing with Ultroser G 1 were lower than those with FCS. However, blastocyst rates for CPSR-3 1 were similar to those for serum. In addition, embryos produced in SOF CPSR-3 1 had the same proportion inner cell mass number and total cell number as embryos cultured with FCS. In Experiment 2, a combination of serum replacements during different periods showed that treatment before the ®ve-to eight-cell stages had no effect on further embryo development. However, treatments up to the morula stage affected blastocyst formation. The concentration of supplement and the timing of its inclusion in culture markedly affected embryo development. The serum replacement CPSR-3 1 can supplement embryo culture with blastocyst rates and quality similar to those for serum. #

Prospectus of cultured meat—advancing meat alternatives

“…Variations among different serum-free media outline the fact that satellite cells from different species have different requirements and respond differentially to certain additives (Dodson et al 1996). Ultroser G is an example of a commercially available serum substitute containing growth factors, binding proteins, adhesin factors, vitamins, hormones, mineral trace elements and has been designed specially to replace fetal bovine serum for growth of anchorage-dependent cells in vitro (Duque et al 2003). Benjaminson et al (2002) succeeded in using a serum-free medium made from maitake mushroom extract that achieved higher rates of growth than fetal bovine serum and recently it has been shown that lipids such as sphingosine-1-phosphate can replace serum in supporting the growth and differentiation of embryonic tissue explants.…”

Macromolecular source as dependent on osmotic pressure and water source: effects on bovine in vitro embryo development and quality

“…Usually, culture media are added with protein supplements such as fetal calf serum (FCS) or bovine serum albumin (BSA) which can contribute amino acids to the medium [13]. These protein sources can be substituted by commercial replacements [14] or synthetic macromolecules such as polyvinyl-alcohol (PVA), a compound seemingly without biological activity, which allows one to obtain defined culture conditions. Although FCS and BSA can introduce viral contaminants [15], and bacterial endotoxins [16,17] and show variability among batches [17], protein has an important protective effect against heavy metals and toxic substances, which can be present in water [18] and/or oil used to cover culture medium [19,20].…”

The Use of Embryonic Stem Cell Derived Bioactive Material as a New Protein Supplement for the In Vitro Culture of Bovine Embryos

“…57: [346][347][348][349][350][351][352][353][354]2011) uccessful preimplantation embryo development in vitro requires a culture environment comparable to in vivo conditions; specifically, this entails using an effective basic culture medium, various protein supplements and a coculture system with a somatic cell feeder layer [1,2]. Almost all media used for bovine embryo development in vitro contain bovine serum albumin (BSA) and fetal bovine serum (FBS) as a source of protein, and recently, serum replacement (SR) or a synthetic serum substitute have also been used [3,4]. Serum provides energy substrates, amino acids, vitamins, growth factors and heavy metal chelators [5].…”