Use of ultrasonography to determine the structural and functional state of ovaries of donor embryo cows

Abstract

Purpose: Assessment of the possibility of using ultrasonography for early prediction of the response of the functional activity of the ovaries to the introduction of some hormonal drugs at ET.Materials and methods. The study was conducted on 42 cows of various breeds (Holstein, Ayrshire, Black-and-white breed of cows) — potential donors of egg and embryos in several farms of various organizational and legal forms of ownership of the Saratov and Volgograd regions of the Russian Federation. Cow donors are selected taking into account anamnestic data. To assess the full response of the ovaries, two experiments were produced to carry out the stimulation of supersuvulation: the first study was introduced on the 10th day of the estrous cycle, 9 animals of follitropine on the developed protocol, and 7-animals — «plus» and the second study — for 11 days of the estrous cycle 8 — animals injected follitropine, and 6 cows — «plus» in the 4-day protocol. The synchronization of the estrus was carried out according to the generally accepted protocol. For morphological studies, the donor cows were extracted by ovaries and studied their physical characteristics, such as volume, weight, thickness, width, gonad length, the size of yellow bodies and follicles. For a more detailed study, ultrasonography was carried out using a Mindray DP 10 VET portable scanner with a rectilinear rectal sensor 7.5 MHz by 10-11 (before the administration of the drug) and zero (after the introduction of the follicularity of the hormonal preparation) of the estrous cycle days.Results. The average rate of ovaries before the introduction of drugs, on the 10th day of the estrous cycle, was 11.103±2.275 cm3, the volume of yellow bodies — 7,122±3.435 cm3. The average volume of yellow bodies to the ovary was 48.7±1.3%. The level of supervision reaction depends on the quality of the yellow body in the middle of the L-phase, at the time preceding the beginning of the gonadotropic stimulation from donor cows.Conclusion. Based on the data obtained, it can be concluded that the visual analysis of sleepograms is an acceptable method for predicting the suitability of donor cows prior to the beginning of animal treatment with gonadotropic drugs and can be used as the animal selection criterion into a group of potential donors. Using ultrasound echography, relevant data reflecting the morphological and functional state of cows ovaries were obtained.