Use of urinary metabolomics to evaluate the effect of hyperuricemia on the kidney

Huang, Chi‐Chang; Lou, Bih‐Show; Hsu, Feng-Lin; Hou, Chia-Chung

doi:10.1016/j.fct.2014.08.017

Cited by 24 publications

(16 citation statements)

References 45 publications

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“…Previous studies have consistently demonstrated the dysfunctional energy metabolism in patients with hyperuricemia as well as in animal models [ 22 , 24 – 26 ]. Important tricarboxylic acid (TCA) cycle intermediate metabolites such as succinate, citrate, and fumarate were downregulated in urine of gout patients and hyperuricemic mouse [ 27 , 28 ]. Hyperuricemic rats in our study exhibited an elevated level of β -glucose in serum together with a decreased level of succinate in urine as compared with normal rats.…”

Section: Discussionmentioning

confidence: 99%

NMR-Based Metabonomic Study Reveals Intervention Effects of Polydatin on Potassium Oxonate-Induced Hyperuricemia in Rats

Han

Gong

Zhong

et al. 2020

Oxidative Medicine and Cellular Longevity

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Previous studies have disclosed the antihyperuricemic effect of polydatin, a natural precursor of resveratrol; however, the mechanisms of action still remain elusive. The present study was undertaken to evaluate the therapeutic effects and the underlying mechanisms of polydatin on potassium oxonate-induced hyperuricemia in rats through metabonomic technology from a holistic view. Nuclear magnetic resonance (NMR) spectroscopy was applied to capture the metabolic changes in sera and urine collected from rats induced by hyperuricemia and polydatin treatment. With multivariate data analysis, significant metabolic perturbations were observed in hyperuricemic rats compared with the healthy controls. A total of eleven and six metabolites were identified as differential metabolites related to hyperuricemia in serum and urine of rats, respectively. The proposed pathways primarily included branched-chain amino acid (BCAA) metabolism, glycolysis, the tricarboxylic acid cycle, synthesis and degradation of ketone bodies, purine metabolism, and intestinal microflora metabolism. Additionally, some metabolites indicated the risk of renal injury induced by hyperuricemia. Polydatin significantly lowered the levels of serum uric acid, creatinine, and blood urea nitrogen and alleviated the abnormal metabolic status in hyperuricemic rats by partially restoring the balance of the perturbed metabolic pathways. Our findings shed light on the understanding of the pathophysiological process of hyperuricemia and provided a reference for revealing the metabolic mechanism produced by polydatin in the treatment of hyperuricemia.

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Section: Discussionmentioning

confidence: 99%

NMR-Based Metabonomic Study Reveals Intervention Effects of Polydatin on Potassium Oxonate-Induced Hyperuricemia in Rats

Han

Gong

Zhong

et al. 2020

Oxidative Medicine and Cellular Longevity

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“…Utilizing a metabolomics approach to understand disease mechanisms and dissect the mechanisms of action of TCM has been a key focus of recent TCM research (Hu and Xu ; Liu et al , ). Recent studies have used various techniques, such as high‐performance liquid chromatography–diode array detection (Liu, Yu, Sun, & Di, ), liquid chromatography–mass spectroscopy (Xu et al , ) and gas chromatography–mass spectroscopy (Huang, Lou, Hsu, & Hou, ), to understand the mechanism of hyperuricemia using urine samples and a metabolomic approach. To some extent, urine metabolomics is susceptible to a dilution factor (depending upon the amount of liquid intake; Walsh, Brennan, Malthouse, Roche, & Gibney, ).…”

Section: Introductionmentioning

confidence: 99%

Metabolomic profiles delineate the effect of Sanmiao wan on hyperuricemia in rats

Jiang¹,

Qian²,

Ding

et al. 2016

Biomedical Chromatography

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A serum metabolomic method based on ultra-high-performance liquid chromatography coupled with mass spectrometry was developed to characterize hyperuricemia-related metabolic profiles and delineate the mechanism of Sanmiao wan (SMW), a traditional Chinese medicine (TCM), in treating hyperuricemic rats. With partial least-squares discriminant analysis for classification and selection of biomarkers, 13 potential biomarkers in mouse serum were identified in the screen, primarily involved in purine metabolism, arginine and proline metabolism, citrate cycle, phenylalanine metabolism, tryptophan metabolism and glycerophospholipid metabolism. Taking these potential biomarkers as screening indexes, SMW could reverse the pathological process of hyperuricemia through partially regulating the perturbed metabolic pathway except for glycerophospholipid metabolism. Our results showed that a metabolomic approach offers a useful tool to identify hyperuricemia-related biomarkers and provides a new methodological cue for systematically dissecting the underlying efficacies and mechanisms of TCM in treating hyperuricemia.

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“…However, current metabonomic studies associated with HUA are mainly pay attention to the total relevant metabolites rather than lipids, and the lipid biomarkers should be subjected to further study [10,11]. Lipidomics emphasis on the the determination of lipid molecular composition in cells, biofluids, tissue, or whole organism and could be a puissant tool to provide a new insight into the change of lipid metabolism in HUA and the anti-HUA mechanism of Plantaginis semen [12].…”

Section: Resultsmentioning

confidence: 99%

Lipidomics Study of the Therapeutic Mechanism of Plantaginis Semen in Potassium Oxonate-Induced Hyperuricemia Rat

Shi

Yang

Wang

et al. 2020

Preprint

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BackgroundPlantaginis semen has been widely used as folk medicine and health care food against hyperuricemia (HUA) and gout, but little was known about its pharmacological mechanism. MethodsThe model was established by potassium oxonate intragastric administration. 42 Sprague-Dawley (SD) male rats were randomly divided into the control group, model group, benzbromarone group (10 mg/kg) and three Plantaginis semen groups (n = 7). The Plantaginis semen groups were treated orally with Plantaginis semen at 0.9375, 1.875 and 3.75 g/kg for 28 days. The levels of serum uric acid (UA), creatinine (Cr), triacylglycerol (TG) and tumor necrosis factor-α (TNF-α) were detected using enzyme-linked immunosorbent assay kits. Ultra performance liquid chromatography quadrupole time of flight mass spectrometry (UPLC-Q-TOF/MS) was used as the basis for serum lipidomics analysis, and orthogonal partial least squares discriminant analysis (OPLS-DA) was carried out for the pattern recognition and characteristic metabolites identification. The relative levels of critical regulatory factors of urate anion transporter 1(URAT1) and phosphatidylinositol 3-kinase/ protein kinases B (PI3K/Akt) were determined by quantitative real-time polymerase chain reaction (RT-qPCR). ResultsCompared with the model group, the levels of serum UA, Cr, and TG were significantly (p<0.01) decreased in benzbromarone and three Plantaginis semen groups and the level of serum TNF-α was significantly (p<0.05) decreased in benzbromarone and low dose of Plantaginis semen group. With lipidomics analysis, significant lipid metabolic perturbations were observed in HUA rats, 13 metabolites were identified as potential biomarkers and glycerophospholipid metabolism pathway was mostly affected. These perturbations can be partially restored via treatment of benzbromarone and Plantaginis semen. Additionally, the URAT1 and PI3K/Akt mRNA expression levels were significantly decreased (p<0.05) after treatment with benzbromarone and high dose of Plantaginis semen. ConclusionsPlantaginis semen had significant anti-HUA, anti-inflammatory and renal protection effects and could attenuate potassium oxonate-induced HUA through regulation of lipid metabolism disorder. Trial registrationNot applicable

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Use of urinary metabolomics to evaluate the effect of hyperuricemia on the kidney

Cited by 24 publications

References 45 publications

NMR-Based Metabonomic Study Reveals Intervention Effects of Polydatin on Potassium Oxonate-Induced Hyperuricemia in Rats

NMR-Based Metabonomic Study Reveals Intervention Effects of Polydatin on Potassium Oxonate-Induced Hyperuricemia in Rats

Metabolomic profiles delineate the effect of Sanmiao wan on hyperuricemia in rats

Lipidomics Study of the Therapeutic Mechanism of Plantaginis Semen in Potassium Oxonate-Induced Hyperuricemia Rat

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