Use of waste canola oil as a low-cost substrate for rhamnolipid production using Pseudomonas aeruginosa

Abstract

Rhamnolipids are glycolipid biosurfactants that are primarily produced by Pseudomonas aeruginosa that have gained a great deal of interest for their numerous industrial applications and environmentally friendly properties. In this study, we explored the potential of waste canola oil as a low-cost and environmentally friendly substrate for the production of rhamnolipids by P. aeruginosa . Four different 2 3 full factorial designs were used to assess the effect of three independent factors on rhamnolipid production, including carbon source (canola oil and waste canola oil), nitrogen source [(NH 4 ) 2 SO 4 and NaNO 3 ] and production time (7 and 14 days). The highest observed yield was 3585.31 ± 66.24 mg/L when P. aeruginosa was cultured for 14 days with 3% v/v waste canola oil and 4 g/L of NaNO 3 . The nitrogen source proved to be a crucial factor, as the use of NaNO 3 rather than (NH 4 ) 2 SO 4 led to a 30-fold increase in production yield. The observed yield when waste canola oil was used was similar to, and even slightly higher than, that obtained using canola oil. Our results showed that waste canola oil has great potential for use as a carbon source for rhamnolipid production by P. aeruginosa , thus paving the way for the development of a low-cost, efficient, and environmentally friendly bioprocess for the production of rhamnolipids. Electronic supplementary material The online version of this article (10.1186/s13568-019-0784-7) contains supplementary material, which is available to authorized users.

Characterization and Cytotoxic Effect of Biosurfactants Obtained from Different Sources

“…For instance, canola oil, soybean oil, waste frying oil, or hydrolyzates from cellulosic or hemicellulosic fractions of vine-trimming wastes have been proposed as a less expensive option compared to traditional culture medias. 13 − 17 …”

Sustainable Production of Microbial Isoprenoid Derived Advanced Biojet Fuels Using Different Generation Feedstocks: A Review

“…Cultivation in a bubble column reactor resulted in a maximum carotene titer of 980 mg/L. The gram-negative bacterium, Pseudomonas aeruginosa, has also been cultivated on a number of waste oils for the production of biosurfactants (Pérez-Armendáriz et al, 2019). An impressive rhamnolipid titer of 3.6 g/L was achieved when the strain was grown on media containing just waste canola oil and a combination of four salts (Pérez-Armendáriz et al, 2019).…”

“…The gram-negative bacterium, Pseudomonas aeruginosa, has also been cultivated on a number of waste oils for the production of biosurfactants (Pérez-Armendáriz et al, 2019). An impressive rhamnolipid titer of 3.6 g/L was achieved when the strain was grown on media containing just waste canola oil and a combination of four salts (Pérez-Armendáriz et al, 2019). Although research into the microbial conversion of waste cooking oil into isoprenoid products is in its infancy, its low cost and high abundance renders it an attractive feedstock for the sustainable production of advanced biojet fuels.…”

Screening for efficient nitrogen sources for overproduction of the biomass of the functionally probiotic L. plantarum strain RPR42 in a cane molasses-based medium

“…However, in case of cheese whey, 12 additional duplicate experiments were performed to find the optimal formula of cane molasses + cheese whey (Additional file 1: Table S1). The measured response variable was biomass production, which was expressed as dry biomass (g/L) (Lim et al 2018;Pérez-Armendáriz et al 2019).…”