Usefulness and validation of a coproantigen test for dog echinococcosis screening in the consolidation phase of hydatid control in Neuquén, Argentina

Pierangeli, Nora Beatriz; Soriano, Silvia Viviana; Roccia, Irene; Bergagna, H.F.J.; Lazzarini, Lorena Evelina; Celescinco, A.; Kossman, Alejandra; Saiz, M.S.; Basualdo, Juan Ángel

doi:10.1016/j.parint.2010.05.004

Cited by 26 publications

(21 citation statements)

References 27 publications

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“…In recent years, several coproELISAs have been standardized for use in existing control programmes in endemic areas of the world (Guarnera, Santillan, Botinelli, & Franco, ; Jara et al, ; Morel et al, ; Pierangel et al, ) and they have been included in the surveillance systems of the countries, such as Peru, Argentina, Uruguay, China and Kyrgyzstan (Centro Panamericano Fiebre Aftosa, ; Irabedra et al, ; van Kesteren, Mastin, Torgerson, Mytynova, & Craig, ; Larrieu & Zanini, ).…”

Section: Ce Surveillancementioning

confidence: 99%

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Control of cystic echinococcosis: Background and prospects

Larrieu

Gavidia

Lightowlers

2019

Zoonoses and Public Health

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Cystic echinococcosis (CE), caused by Echinococcus granulosus, is a chronic and debilitating zoonotic larval cestode infection in humans, which is principally transmitted between dogs and domestic livestock, particularly sheep. Human CE occurs in almost all pastoral communities and rangeland areas of the underdeveloped and developed world. Control programmes against CE have been implemented in several endemic countries to reduce or eliminate the disease. New Zealand and Tasmania are examples of some of the first programmes to be undertaken (in insular territories) and which were very successful in the elimination of CE. The advent and proven effectiveness of praziquantel, plus the experience of insular models, produced high expectations for rapid advances in a second generation of control programmes undertaken in continental areas (Argentina, Uruguay and Chile). Nevertheless, only moderate gains in CE control have been made and the impact on prevalence among humans has been slight. A major impediment to the adoption of procedures that were successful in New Zealand and Tasmania has been the requirement to administer praziquantel to dogs in rural areas eight times per year over numerous years. In addition, there have been clear technological improvements made in the diagnosis of canine echinococcosis for surveillance, the genetic characterization of parasite strains and in vaccination against CE infection in livestock. In order to establish new paradigms and appropriate combinations of control strategies, we have carried out a review and discussion of the available control tools and control models. Control strategies must be suitable and sustainable to benefit the Echinococcosis–endemic areas primarily, which at the same time are the poorest regions of the world.

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Section: Ce Surveillancementioning

confidence: 99%

“…In recent years, several coproELISAs have been standardized for use in existing control programmes in endemic areas of the world (Guarnera, Santillan, Botinelli, & Franco, 2000;Jara et al, 2019;Morel et al, 2013;Pierangel et al, 2010)…”

Section: Surveillance In Dogsmentioning

confidence: 99%

Control of cystic echinococcosis: Background and prospects

Larrieu

Gavidia

Lightowlers

2019

Zoonoses and Public Health

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“…28 The sensitivity of our test was higher than previously reported copro-ELISAs that used polyclonal antibodies. 13,[24][25][26] Two false-negative samples were observed in the present study; however, one of these had only one parasite, whereas the other was sampled at 20 days postinfection with E. granulosus. The parasite burden and the postinfection time may have affected detection due to fewer parasitic antigens being produced by a lower number of worms 29 or due to the antigen presentation of immature worms in early infection.…”

Section: Discussionmentioning

confidence: 48%

“…By contrast, other copro-ELISA tests have reported early detection before 10 days postinfection with the use of antibodies to anti-E. granulosus somatic crude proteins obtained by sonication. 13 It may detect more antigens but are less specific as compared with our anti-EGMA obtained by nonionic detergent which probably isolates outer surface proteins instead of somatic proteins.…”

Section: Discussionmentioning

confidence: 89%

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Development and Validation of a Copro-Enzyme–Linked Immunosorbent Assay Sandwich for Detection of Echinococcus granulosus–Soluble Membrane Antigens in Dogs

Jara

Rodríguez

Altamirano

et al. 2019

The American Journal of Tropical Medicine and Hygiene

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Cystic echinococcosis (CE) is a parasitic zoonosis caused by the larval stage of the tapeworm Echinococcus granulosus. Detection of the adult stage in the canine definitive host is essential for estimating infection rates, surveillance and monitoring of CE control programs. This study sought to develop and validate a coproantigen sandwich enzyme-linked immunosorbent assay (copro-ELISA), based on antibodies against E. granulosus-soluble membrane antigens (EGMA), that is capable of distinguishing infected and noninfected dogs. Anti-E. granulosus polyclonal immunoglobulin G antibodies were obtained from rabbit antiserum against EGMA. Optimization of the test was performed with 51 positive and 56 negative stool samples of canine echinococcosis. Specificity, sensitivity, cross-reactivity, intra-and inter-assay precision, and over time detection were evaluated. According to the receiver operating characteristic analysis, the diagnostic sensitivity and specificity were 96.1% (CI: 85.9-99.6) and 98.2% (CI: 89.5-100), respectively. Negative and positive predictive values were 96.5% (CI: 91.7-100) and 98% (CI: 94.1-100), respectively. No cross-reactivity with Taenia hydatigena, Dipylidium caninum, or Toxocara canis was observed. Intra-and inter-assay repeatability showed values of less than 15% of the variation coefficient. The over time detection was from 20 to 27 days postinfection with E. granulosus. The copro-ELISA based on EGMA detection offers a simplified in-house development of diagnostic testing. This assay showed high specificity and sensitivity and had no cross-reactivity with other parasites. Further studies and development of this test in a kit format may be useful for the detection of active infection in dogs living in CE endemic regions.

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Epidemiological studies on Echinococcus in Pampas fox (Lycalopex gymnocercus) and European hare (Lepus europaeus) in Buenos Aires province, Argentina

et al. 2013

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In Argentina, hydatid disease caused by Echinococcus granulosus is widespread. The south of Buenos Aires province, Argentina, is one of the three regions where hydatidosis is endemic. Although domestic dogs and sheep are considered to be the main hosts for E. granulosus, the potential role of wildlife in the local transmission of E. granulosus has not been investigated. The aim of this study was to estimate the hydatidosis/echinococcosis prevalence in European hare (Lepus europaeus) and Pampas fox (Lycalopex gymnocercus), two abundant species with a strong predator-prey relationship in rural areas of Buenos Aires province using different diagnostic tests. A total of 61 fox intestines were examined, finding that 52 (85.2%) harbored at least one helminth species. However, no adult or immature form of Echinococcus sp. was found in the intestinal contents. Coproparasitological analysis and Copro-ELISA followed by Copro-PCR were used as supplementary diagnostic tests. Only one (1.7%) of 59 fecal samples was positive to Taeniidae eggs by coproparasitological analysis, but this same sample was negative by the Copro-ELISA test. The analysis by Copro-ELISA showed 6 of 57 (10.6%) positive samples, but the Copro-PCR tests carried out on these samples were negative to E. granulosus. A total of 6,808 lungs, 3,576 livers, and 3,542 hearts of hunted hares were examined and palpated, but no structure resembling hydatid cysts were detected. Our results suggest that hares and Pampas foxes are not currently important wild reservoirs of E. granulosus in the studied area.

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Usefulness and validation of a coproantigen test for dog echinococcosis screening in the consolidation phase of hydatid control in Neuquén, Argentina

Cited by 26 publications

References 27 publications

Control of cystic echinococcosis: Background and prospects

Control of cystic echinococcosis: Background and prospects

Development and Validation of a Copro-Enzyme–Linked Immunosorbent Assay Sandwich for Detection of Echinococcus granulosus–Soluble Membrane Antigens in Dogs

Epidemiological studies on Echinococcus in Pampas fox (Lycalopex gymnocercus) and European hare (Lepus europaeus) in Buenos Aires province, Argentina

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