Usefulness of HPLC Assay for Early Detection of Microalbuminuria in Chronic Kidney Disease

Horikoshi, Satoshi; Okuda, Masumi; Nishimura, Emi K.; Ohsawa, Isao; Suzuki, Yusuke; Shimizu, Yoshio; Hamada, Chikuma; Tomino, Yasuhiko

doi:10.1002/jcla.21608

Cited by 5 publications

(5 citation statements)

References 25 publications

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“…From a clinical point of view, it has been suggested that elevated UAE, determined by HPLC, can precede the development of microalbuminuria measured by conventional methods (IRAE), and therefore it could be used for early detection [18,37]. Moreover, some studies have suggested that it could help identify more people at risk of death or CV disease than conventional methods [33,38].…”

Section: Discussionmentioning

confidence: 99%

Immune-unreactive urinary albumin as a predictor of cardiovascular events: the Hortega Study

Martínez

Pichler

Ruiz

et al. 2018

Nephrology Dialysis Transplantation

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Section: Discussionmentioning

confidence: 99%

Immune-unreactive urinary albumin as a predictor of cardiovascular events: the Hortega Study

Martínez

Pichler

Ruiz

et al. 2018

Nephrology Dialysis Transplantation

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“…19), which shows that it has the highest sensitivity because of being strongest catalytic amplification, so it was selected to measure mAlb. Compared with the reported mAlb analysis method (Table S3), [31][32][33][34][35][37][38][39] this method is highly sensitive and easy to operate, and it is one of the best methods for detecting mAlb.…”

Section: Working Curvementioning

confidence: 99%

“…30 radioimmunoassay, 32 ELISA, 33 turbidity analysis, 34 highperformance liquid chromatography, and so on. 35 In this paper, a new gold nanoplasmon molecular spectral platform was established for rapid and selective quantitative detection of trace mAlb based on the immunoreaction-regulation of the GONR catalytic AuNP reaction.…”

Section: Introductionmentioning

confidence: 99%

A new SERS strategy for quantitative analysis of trace microalbuminuria based on immunorecognition and graphene oxide nanoribbon catalysis

Wen

Liang

et al. 2018

IJN

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BackgroundMicroalbuminuria (mAlb) detection is essential for the diagnosis and prognosis of nephrotic patients and hypoproteinemia. In this article, we develop a new surface-enhanced Raman scattering (SERS) quantitative analysis method to detect mAlb in urine.MethodsCombined the mAlb immunoreaction with gold nanoreaction of graphene oxide nanoribbons (GONR)-HAuCl4-H2O2, and used Victoria blue B (VBB) as molecular probe with a SERS peak at 1,615 cm−1, a new SERS strategy for quantitative analysis of trace mAlb in urine was established.ResultsThe linear range of SERS quantitative analysis method is from 0.065 to 2.62 ng/mL, with a detection limit of 0.02 ng/mL. The SERS method was applied to analysis of mAlb in urine with good accuracy and reliability, the relative standard deviation is 0.49%–2.28% and the recovery is 96.9%–109.8%.ConclusionThis study demonstrated that the new SERS quantitative analysis method is of high sensitivity, good selectivity and simplicity. It has been applied to analysis of mAlb in urine, with satisfactory results.

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“…This sensitive method to measure urinary protein will be useful for the screening of microalbuminuria because proteinuria in early stages of kidney diseases mostly consist of albumin. A high-performance liquid chromatography (HPLC) assay, which we reported as a sensitive method to detect microalbuminuria 4) , is also available but costs the same amount as TIA. The erythrosin B method has a 70% cost savings compared with TIA 3) .…”

Section: New Biomarkers Using Urine and Serum Samplesmentioning

confidence: 99%

New Biomarkers for Diagnosis in Patients with Chronic Kidney Disease (CKD)

Tomino

2015

Juntendo Medical Journal

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In Japan, major causes of end-stage kidney disease (ESKD) are type 2 diabetic nephropathy, IgA nephropathy, and nephrosclerosis. Renal replacement therapies are hemodialysis (HD) and peritoneal dialysis (PD). Since PD patients have shown some complications, such as peritonitis, infections of the exit sites of catheters, and encapsulated peritoneal sclerosis (EPS), it is necessary to determine infections at an early stage of PD. Although a definite diagnosis in patients with chronic kidney disease (CKD) is made through the biopsy of renal tissue and peritoneum, we cannot always perform such diagnostic procedures. Thus, it is necessary to develop non-invasive diagnostic biomarkers prior to biopsies in CKD patients. The objective of this review is to explain the efficacy of new biomarkers in such patients.

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Usefulness of HPLC Assay for Early Detection of Microalbuminuria in Chronic Kidney Disease

Abstract: These results suggest that ACR measurements by HPLC assay are better than TIA for early detection and monitoring of microalbuminuria in patients with diabetes and hypertension.

Cited by 5 publications

References 25 publications

Immune-unreactive urinary albumin as a predictor of cardiovascular events: the Hortega Study

Immune-unreactive urinary albumin as a predictor of cardiovascular events: the Hortega Study

A new SERS strategy for quantitative analysis of trace microalbuminuria based on immunorecognition and graphene oxide nanoribbon catalysis

New Biomarkers for Diagnosis in Patients with Chronic Kidney Disease (CKD)

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