Usefulness of &lt;i&gt;Mycobacterium tuberculosis&lt;/i&gt; Molecular Typing in a Tuberculosis Low-Endemic Agro-Industrial Setting of Brazil

Malaspina, Ana Carolina; Cavalcanti, Hebe Rodrigues; Leite, Clarice Queico Fujimaro; Machado, Silvia Maria Almeida; Viana, Brunilde Helena Jung; Silva, Rosangela Maria Gaspareto; Hage, Eduardo Ferraz; Figueiredo, Walter Machado; Marques, Elisabeth; Ferrazoli, Lucilaine; Arbex, Marcos Abdo; Lessi, Marcio; Fonseca, Leila de Souza; Rigouts, Leen; Saad, Maria Helena Féres

doi:10.7883/yoken.jjid.2008.231

Cited by 8 publications

(1 citation statement)

References 6 publications

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“…East-African-Indian lineage: Brazil and Mexico Strong phylogeographic specificities were observed in different regions of Brazil resulting from different migratory processes that occurred in each region [33,[72][73][74][75]. We highlight the significant presence of the East-African-Indian (EAI) lineage in the northern region, specifically in Pará, in almost 10% of the TB cases.…”

Section: Genotypes Associated With Other Lineages In Latin Americamentioning

confidence: 90%

Genotyping methods and their contributions to the study of tuberculosis dynamic in Latin America

Castillos de Ibrahim das Neves,

Reis,

Xavier Maio

et al. 2023

J Infect Dev Ctries

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Introduction: Mycobacterium tuberculosis genotyping has impacted evolutionary studies worldwide. Nonetheless, its application and the knowledge generated depend on the genetic marker evaluated and the detection technologies that have evolved over the years. Here we describe the timeline of main genotypic methods related to M. tuberculosis in Latin America and the main findings obtained. Methodology: Systematic searches through the PubMed database were performed from 1993 to May 2021. A total of 345 articles met the inclusion criteria and were selected. Results: Spacer oligonucleotide typing (spoligotyping) was the most widely used method in Latin America, with decreasing use in parallel with increasing use of mycobacterial interspersed repetitive unit-variable number tandem repeat (MIRU-VNTR) and whole genome sequencing (WGS). Among the countries, Brazil, Mexico, and Argentina had the most publications, and a considerable part of the articles were in collaboration with Latin American or non-Latin American institutions; a small proportion of studies needed partnerships to perform the genotypic methods. The genotypic methods allowed the identification of M. tuberculosis genotypes with greater capacity for clonal expansion and revealed the predominance of the Euro-American lineage in Latin America. There was a notable presence of the Beijing family in Peru and Colombia. Conclusions: The data obtained demonstrated the importance of expanding collaborative networks of tuberculosis (TB) research groups to countries with low productivity in this area, the commitment of the few Latin American countries to advance TB research, as well as the inestimable value of building a Latin America database, considering ease of population mobility between countries.

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Section: Genotypes Associated With Other Lineages In Latin Americamentioning

confidence: 90%

Genotyping methods and their contributions to the study of tuberculosis dynamic in Latin America

Castillos de Ibrahim das Neves,

Reis,

Xavier Maio

et al. 2023

J Infect Dev Ctries

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Mycobacterium tuberculosis population structure shift in a 5-year molecular epidemiology surveillance follow-up study in a low endemic agro-industrial setting in São Paulo, Brazil

Santos

Gaspareto

Viana

et al. 2013

International Journal of Mycobacteriology

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Starting with 257 outpatients attending the specialized health service for tuberculosis (TB) between 2002 and 2006 in Araraquara, an agro-industrial area with low tuberculosis (TB) incidence in São Paulo state, Brazil, positive mycobacterial cultures were obtained in 130 cases, of which 121 were confirmed as Mycobacterium tuberculosis complex. This report assesses the genetic diversity observed on 69.42% (n=84) of the clinical isolates, for which both spoligotyping and 12-loci MIRU typing data were fully interpretable. In order to monitor changes in the population dynamics of circulating M. tuberculosis strains over time, spoligotypes were compared from this study (n=84) with an earlier study from 1998 to 2001 (n=70 strains); and these two datasets from low-incidence Araraquara area were also compared with a 2-year cohort in the nearby higher-incidence São Paulo city area from 2006 to 2008 (n=93). The results obtained showed that with 58.3% (49/84) of the strains, the Latin-American-Mediterranean (LAM) was the predominant lineage in the present follow-up study; major patterns being SIT42/LAM9 11.9% (10/84), and SIT20/LAM1 10.7% (9/84). As compared with the 1998-2001 period when 40% (28/70) of the isolates belonged to the ill-defined T family, it was replaced by LAM strains between 2002 and 2006 with a visible shift to a population structure characteristic of the metropolitan São Paulo city. Further typing of the follow-up isolates from 2002 to 2006 using 12 loci MIRUs in conjunction with conventional epidemiology did not link this population structure shift to an increase in ongoing transmission or drug-resistance. Instead, it is most probably linked to movements of the important migrant community of Araraquara to higher TB incidence metropolitan areas such as São Paulo city. This is of particular concern owing to the increment in the global burden of LAM strains and the recent association of certain LAM sublineages with multidrug- and extensively drug-resistant TB. These observations suggest the need for further molecular monitoring of the TB population structure and the evaluation of transmission trends amongst migrant workers and other risk groups, such as persons in homeless shelters, in correctional facilities, drug users, and those with HIV infection, etc.

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Avaliação das moléculas com atividade antiTB das plantas do cerrado brasileiro

Prince

Pavan

Sato

et al. 2010

Revista Portuguesa de Pneumologia

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Usefulness of <i>Mycobacterium tuberculosis</i> Molecular Typing in a Tuberculosis Low-Endemic Agro-Industrial Setting of Brazil

Cited by 8 publications

References 6 publications

Genotyping methods and their contributions to the study of tuberculosis dynamic in Latin America

Genotyping methods and their contributions to the study of tuberculosis dynamic in Latin America

Mycobacterium tuberculosis population structure shift in a 5-year molecular epidemiology surveillance follow-up study in a low endemic agro-industrial setting in São Paulo, Brazil

Avaliação das moléculas com atividade antiTB das plantas do cerrado brasileiro

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