Usefulness of toxicological validation of VOCs catalytic degradation by air-liquid interface exposure system

Zallouha, Margueritta Al; Landkocz, Yann; Brunet, Julien; Cousin, Renaud; Genty, Éric; Courcot, Dominique; Siffert, Stéphane; Shirali, Pirouz; Billet, Sylvain

doi:10.1016/j.envres.2016.10.027

Cited by 17 publications

(6 citation statements)

References 28 publications

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“…In exposed workers, RQ values were as high as 3.0, that is, the values obtained in BEAS‐2B cells exposed to toluene (100 ppm). In addition, a significant overexpression of the gene during in vivo or in vitro exposures to benzene, toluene, or xylenes was also measured in the literature (Al Zallouha et al, 2017; Maguin et al, 2006; Sheets, Yost, & Carlson, 2004). Our results are thus in agreement with experimental data obtained after exposure of rats to benzene or toluene (González‐Jasso et al, 2003).…”

Section: Discussionmentioning

confidence: 90%

Toxicological responses of BEAS‐2B cells to repeated exposures to benzene, toluene, m‐xylene, and mesitylene using air–liquid interface method

Meausoone

Landkocz

Cazier

et al. 2020

J of Applied Toxicology

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In order to reduce exposure to toxic chemicals, the European REACH regulation (1907/2006) recommends substituting toxic molecules with compounds that are less harmful to human health and the environment. Toluene is one of the most frequently used solvents in industries despite its toxicity. The objective of this study is to better understand and compare the toxicity of toluene and its homologues in a bronchial cell model. Thus, human bronchial BEAS‐2B cells were exposed to steams of toluene, m‐xylene, mesitylene (1,3,5‐trimethylbenzene), and benzene (20 and 100 ppm). Exposure was carried out using an air–liquid interface (ALI) system (Vitrocell) during 1 h/day for 1, 3, or 5 days. Cytotoxicity, xenobiotic metabolism enzyme gene expression, and inflammatory response were evaluated following cell exposures. BEAS‐2B cell exposure to toluene and its homologues revealed the involvement of major (CYP2E1) and minor metabolic pathways (CYP1A1). A late induction of genes (EPHX1, DHDH, ALDH2, and ALDH3B1) was measured from Day 3 and can be linked to the formation of metabolites. An increase in the secretion level of inflammatory markers (TNF‐α, IL‐6, IL‐8, MCP‐1, and GM‐CSF) was also observed. In parallel, regulation between inflammatory mediators and the expression of transmembrane glycoprotein mucin MUC1 was also studied. This in vitro approach with ALI system points out the relevance of conducting repeated exposures to detect potential late effects. The difference recorded after cell exposure to toluene and its homologues highlights the importance of substitution principle.

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Section: Discussionmentioning

confidence: 90%

Toxicological responses of BEAS‐2B cells to repeated exposures to benzene, toluene, m‐xylene, and mesitylene using air–liquid interface method

Meausoone

Landkocz

Cazier

et al. 2020

J of Applied Toxicology

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“…With the development of photocatalysis, the use of photocatalysts to decompose air pollutants has received increasing attention from researchers [375][376][377][378][379][380][381][382]. Photocatalytic material could adsorb or decompose toxic gases in the atmosphere, reducing the adverse effects of toxic gases on the environment.…”

Section: Air Purificationmentioning

confidence: 99%

Recent Advances and Applications of Semiconductor Photocatalytic Technology

et al. 2019

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Along with the development of industry and the improvement of people’s living standards, peoples’ demand on resources has greatly increased, causing energy crises and environmental pollution. In recent years, photocatalytic technology has shown great potential as a low-cost, environmentally-friendly, and sustainable technology, and it has become a hot research topic. However, current photocatalytic technology cannot meet industrial requirements. The biggest challenge in the industrialization of photocatalyst technology is the development of an ideal photocatalyst, which should possess four features, including a high photocatalytic efficiency, a large specific surface area, a full utilization of sunlight, and recyclability. In this review, starting from the photocatalytic reaction mechanism and the preparation of the photocatalyst, we review the classification of current photocatalysts and the methods for improving photocatalytic performance; we also further discuss the potential industrial usage of photocatalytic technology. This review also aims to provide basic and comprehensive information on the industrialization of photocatalysis technology.

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“…Kết quả nghiên cứu đạt được của chúng tôi có thể làm cơ sở cho những nghiên cứu tiếp theo trong việc xem xét xác định mức độ biểu hiện của gen CYP2E1 làm chỉ số giám sát sinh học cho người lao động có tiếp xúc với benzen, toluen, ethylbenzen, xylen. Kết quả này cũng phù hợp với xu hướng tăng mức độ biểu hiện ở người có tiếp xúc và đề xuất sử dụng mRNA của gen CYP2E1 làm chỉ số giám sát sinh học của Cantu [1,15] và các tác giả khác.…”

Section: Thảo Luậnunclassified

Expression of CYP2E1 gene in paint workers occupationally exposed to organic solvents

Hiền

Nhung²,

Hung³

et al. 2019

NST

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Benzene (B), toluene (T), ethylbenzene (E) and xylene (X) are used commonly in paint industry, so workers who are a high risk of exposure to organic solvent (VOCs). CYP2E1 gene encodes CYP2E1 which plays an important role in the metabolism and bio-activation of volatile organic compounds. When workers expose to VOCs, their body will have a mechanism to metabolize the toxic. The enhancement of mRNA expression of CYP2E1 is a very sensitive and accurate biological marker, which is the basis for the next study to propose the level of mRNA expression of CYP2E1 gene as a human biological indicator to monitor to workers occupationally exposed to VOCs. In this study, we studied the level of mRNA expression of CYP2E1 gene from 118 participants that including 73 workers of exposed group from the paint factories and 45 workers of non-exposed group from garment factories by using Realtime-PCR with SYBR Green – an asymmetrical cyanine dye used as a nucleic acid stain in molecular biology. The initial results, showed that the exposed group had a higher mRNA expression level of CYP2E1 than the non-exposed group approximately 10.47 times, and this difference was statistically significant (P value < 0.05). And the age and the duration of exposure to organic solvents do not affect the expression level of CYP2E1. Keywords CYP2E1, the metabolisms of volatile organic compounds, mRNA expression of CYP2E1. References [1] A. Mendoza-Cantu, F. Castorena-Torres, M. Bermudez De Leon et al., Occupational toluene exposure induces cytochrome P450 2E1 mRNA expression in peripheral lymphocytes, Environmental Health Perspectives, 114 (2006) 494 - 499. https://doi.org/10.1289/ehp.8192.[2] J.H. Hartman, G. Boysen and G.P. Miller, CYP2E1 metabolism of styrene involves allostery, Drug Metabolism and Disposition, 40 (2012) 1976-1983. https://doi.org/10.1124/dmd. 112.046698. [3] S.M. Zhu, X.F. Ren, J.X. Wan et al., Evaluation in vinyl chloride monomer (VCM) - exposed workers and the relationship between liver lesions and gene polymorphisms of metabolic enzymes, World Journal of Gastroenterology,11 (2005) 5821 -5827. 10.3748/wjg.v11.i37.5821.[4] J. Wan, J. Shi, L. Hui et al., Association of genetic polymorphisms in CYP2E1, MPO, NQO1, GSTM1, and GSTT1 genes with benzene poisoning, Environmental Health Perspectives, 110 (2002), 1213-1218. https://doi.org/10.1289/ ehp.021101213.[5] U. Bernauer, B. Vieth, R. Ellrich et al., CYP2E1 - dependent benzene toxicity: the role of extrahepatic benzene metabolism, Archives of Toxicology, 73 (1999) 189 -196. https://doi.org/ 10.1007/s002040050605. [6] P.L. Sheets, G.S. Yost and G.P. Carlson. Benzene metabolism in human lung cell lines BEAS‐2B and A549 and cells overexpressing CYP2F1, Journal of Biochemical and Molecular Toxicology, 18 (2004) 92-99. https://doi.org/10. 1002/jbt.20010.[7] V. Nedelcheva, I. Gut, P. Souček et al., Metabolism of benzene in human liver microsomes: individual variations in relation to CYP2E1 expression, Archives of Toxicology, 73 (1999) 33-40. https://doi.org/10.1007/s0020400 50583.[8] W. Tassaneeyakul, D.J. Birkett, J.W. Edwards et al., Human cytochrome P450 isoform specificity in the regioselective metabolism of toluene and o-, m-and p-xylene, Journal of Pharmacology and Experimental Therapeutics, 276 (1996) 101 - 108. 10.1163/2211730x96x00063[9] A.-H. Wang, S.-M. Zhu, Y.-L. Qiu et al., CYP2E1 mRNA expression, genetic polymorphisms in peripheral blood lymphocytes and liver abnormalities in Chinese VCM-exposed workers, International Journal of Occupational Medicine and Environmental Health, 21 (2008), 141 - 146. 10.2478/v10001-008-0016-x.[10] J. Zhang, Y. Lihong, G. Liang et al., Detection of CYP2E1, a genetic biomarker of susceptibility to benzene metabolism toxicity in immortal human lymphocytes derived from the Han Chinese population, Biomedical and Environmental Sciences, 24 (2011) 300-309. https://doi.org/10. 3967/0895-3988.2011.03.014[11] American Conference of Industrial Hygienists, Guide to Occupational Exposeure Values, ACGIH, Cincinnati, 2018.[12] Bộ Y Tế, Quyết định của Bộ trưởng Bộ Y tế về việc ban hành 21 tiêu chuẩn vệ sinh lao động, 05 nguyên tắc và 07 thông số vệ sinh lao động, 2002.[13] American Conference of Industrial Hygienists, Threshold Limit Value for Chemical Substances and Physical Agents and Biological Exposure Indices, ACGIH, Cincinnati, 2018.[14] Bộ Y Tế, Thông tư 28/2006/TT- BYT hướng dẫn quản lý bệnh bệnh nghề nghiệp, 2016.[15] M. Al Zallouha, Y. Landkocz, J. Brunet et al., Usefulness of toxicological validation of VOCs catalytic degradation by air-liquid interface exposure system", Environmental Research, 152 (2017) 328-335. https://doi.org/10.1016/j.envres. 2016.10.027

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Usefulness of toxicological validation of VOCs catalytic degradation by air-liquid interface exposure system

Cited by 17 publications

References 28 publications

Toxicological responses of BEAS‐2B cells to repeated exposures to benzene, toluene, m‐xylene, and mesitylene using air–liquid interface method

Toxicological responses of BEAS‐2B cells to repeated exposures to benzene, toluene, m‐xylene, and mesitylene using air–liquid interface method

Recent Advances and Applications of Semiconductor Photocatalytic Technology

Expression of CYP2E1 gene in paint workers occupationally exposed to organic solvents

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