Usefulness of Toxoplasma gondii recombinant antigens (GRA1, GRA7 and SAG1) in an immunoglobulin G avidity test for the serodiagnosis of toxoplasmosis

Pietkiewicz, Halina; Hiszczyńska-Sawicka, Elżbieta; Kur, Józef; Petersen, Eskild; Nielsen, Henrik Vedel; Paul, Margaret M.; Stankiewicz, M.; Myjak, P

doi:10.1007/s00436-006-0265-1

Cited by 53 publications

(54 citation statements)

References 26 publications

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“…The rROP2 antigen was of good quality as evaluated by immunoblot. rROP2 was further used as an antigen for the diagnosis of toxoplasmosis, although the results were disappointing ( VAN GELDER et al, 1993, MARTIN et al, 1998, AUBERT et al, 2000, NIGRO et al, 2003, ALTCHEH et al, 2006, PIETKIEWICZ et al, 2007. Our data was similar to those reported by VAN GELDER et al (1993) and those obtained with the same rROP2 source (MARTIN et al,.…”

Section: Discussionsupporting

confidence: 84%

“…Several other recombinant antigens give similar results, but they were not included in the commercial assays. As the results were similar but weaker than for whole extract ELISA, attempts have been made to introduce a mixture of several recombinant antigens or chimeric proteins composed of several epitopes of diverse T. gondii proteins (BUFFOLANO et al, 2005;BEGHETTO et al, 2006;PIETKIEWICZ et al, 2007). However, all attempts have given similar results without improving upon the results of the whole extract ELISA.…”

Section: Discussionmentioning

confidence: 99%

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Serology using rROP2 antigen in the diagnostic of toxoplasmosis in pregnant women

Macre¹,

Pires²,

Meireles³

et al. 2009

Rev. Inst. Med. trop. S. Paulo

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SUMMARYToxoplasma gondii causes severe fetal disease during acute infection in pregnant women, thus demanding early diagnosis for effective treatment and fetus preservation. Fetal tests are inefficient and risky, and diagnosis is based on maternal IgM serology, which had weak screening ability due to increased sensitivity, with alternative IgG avidity tests. Here, we performed ELISA and avidity assays using a recombinant T. gondii antigen, rROP2, in samples from 160 pregnant women screened from a large public hospital who were referred due to positive IgM assays. IgG serology and avidity assays were compared using whole T. gondii extract or rROP2. ELISA IgG detection with rROP2 showed good agreement with assays performed with T. gondii extract, but rROP2 IgG avidity assays were unrelated to whole extract antigen IgG avidity, regardless of the chaotrope used. These data show that avidity maturation is specific to individual antigen prevalence and immune response during infection. ELISA rROP2 IgG assays may be an alternative serological test for the diagnosis of toxoplasmosis during pregnancy, although our data do not support their use in avidity assays.

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Section: Discussionsupporting

confidence: 84%

Section: Discussionmentioning

confidence: 99%

Serology using rROP2 antigen in the diagnostic of toxoplasmosis in pregnant women

Macre¹,

Pires²,

Meireles³

et al. 2009

Rev. Inst. Med. trop. S. Paulo

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“…Recently, an indirect ELISA test with purified tachyzoite surface antigen SAG1 has been proposed (Hosseininejad et al, 2009). SAG1 antigen is stage-specific and only detectable in the tachyzoite stage (Pietkiewicz et al, 2007).…”

Section: Modified Agglutination Test Immunofluorescence Antibody Tesmentioning

confidence: 97%

Toxoplasma in Animals, Food, and Humans: An Old Parasite of New Concern

Cenci‐Goga

Rossitto

Sechi

et al. 2011

Foodborne Pathogens and Disease

142

106

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All hosts, including humans, can be infected by any one of the three forms of the parasite Toxoplasma gondii that correspond to three morphological stages: tachyzoite, bradyzoite, and sporozoite form. Felids are definitive hosts for T. gondii, which is an intracellular pathogen that infects a wide range of warm-blooded intermediate hosts. Toxoplasmosis is a disease where the interest of the diverse medical and veterinary specialties converge. Awareness needs to be increased that toxoplasmosis can induce clinical disease not only in immunocompromised patients or through congenital infections, but also in healthy patients. This is a review article that aims at illustrating why toxoplasmosis should be regarded a veterinary public health issue and how veterinary practitioners can contribute in controlling the infection.

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“…Therefore, detection of GRA7 antibodies would be expected to be a good candidate to use for serodiagnosis. Several rGRA antigens such as rGRA2 (Golkar et al, 2007;HolecGąsior et al, 2009), rGRA6 (Aubert et al, 2000Golkar et al, 2008), rGRA7 (Aubert et al, 2000Pietkiewicz et al, 2004;Pfrepper et al, 2005;Pietkiewicz et al, 2007), and rGRA8 (Pfrepper et al, 2005;Gatkowska et al, 2006;Babaie et al, 2009) have been proposed as markers to indicate acute infections. rGRA4 and rGRA7, but not rROP2, have been shown to be valuable in differentiating acute and chronically infected individuals for both adult and congenital toxoplasmosis (Nigro et al, 2003;Altcheh et al, 2006).…”

Section: Dense Granule Antigens (Gra)mentioning

confidence: 99%

“…The combinations of recombinant antigens that could be used to distinguish between acute and chronic infections are summarized in Table 2. Li et al, 2000 rGRA8, rSAG2, rGRA2, andrGRA7 Beghetto et al, 2003 rGRA3, rGRA7, rMIC3, and rSAG1 Pietkiewicz et al, 2007 rGRA1, rGRA7 and rSAG1 Table 2. Selected combination of recombinant antigens that use to distinguish between acute and chronic infections.…”

Section: Combinations Of Recombinant Antigensmentioning

confidence: 99%