User‐friendly cell patterning methods using a polydimethylsiloxane mold with microchannels

Funano, Shun‐ichi; Tanaka, Nobuyuki; Tanaka, Yo

doi:10.1111/dgd.12637

Cited by 12 publications

(7 citation statements)

References 56 publications

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“…Lastly, several popular methods of microfluidic chip fabrication are available to produce the Rotation-Chip, including polydimethylsiloxane (PDMS) molding, 29 glass etching, 30 CNC machining, 31 and injection molding. 32 Though conventional and inexpensive, PDMS molding has properties that were unsuitable for the shear strength and overall rigidity requirements of our application.…”

Section: ■ Results and Discussionmentioning

confidence: 99%

Superhydrophobic Rotation-Chip for Computer-Vision Identification of Drug-Resistant Bacteria

Peng

Yuqing

et al. 2023

ACS Appl. Mater. Interfaces

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The transport, distribution, and mixing of microfluidics often require additional instruments, such as pumps and valves, which are not feasible when operated in point-of-care (POC) settings. Here, we present a simple microfluidic pathogen detection system known as Rotation-Chip that transfers the reagents between wells by manually rotating two concentric layers without using external instruments. The Rotation-Chip is fabricated by a simple computer numerical control (CNC) machining process and is capable of carrying out 60 multiplexed reactions with a simple 30 or 60° rotation. Leveraging superhydrophobic coating, a high fluid transport efficiency of 92.78% is achieved without observable leaking. Integrated with an intracellular fluorescence assay, an on-chip detection limit of 1.8 × 106 CFU/mL is achieved for ampicillin-resistant Escherichia coli (E. coli), which is similar to our off-chip results. We also develop a computer vision method to automatically distinguish positive and negative samples on the chip, showing 100% accuracy. Our Rotation-Chip is simple, low-cost, high-throughput, and can display test results with a single chip image, making it ideal for various multiplexing POC applications in resource-limited settings.

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Section: ■ Results and Discussionmentioning

confidence: 99%

Superhydrophobic Rotation-Chip for Computer-Vision Identification of Drug-Resistant Bacteria

Peng

Yuqing

et al. 2023

ACS Appl. Mater. Interfaces

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show abstract

“…Lastly, several popular methods of microfluidic chip fabrication are available to produce the Rotation-Chip, including polydimethylsiloxane (PDMS) molding 30 , glass etching 31 , CNC machining 32 , and injection molding 33 . Though conventional and inexpensive, PDMS molding has properties that were unsuitable for the shear strength and overall rigidity requirements of our application.…”

Section: Resultsmentioning

confidence: 99%

Superhydrophobic Rotation-Chip for Computer-Vision Identification of Drug-Resistant Bacteria

Peng

Yuqing³

et al. 2023

Preprint

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The transport, distribution, and mixing of microfluidics often require additional instruments, such as pumps and valves, which are not feasible when operated in point-of-care (POC) settings. Here, we present a simple microfluidic pathogen detection system known as Rotation-Chip that transfers the reagents between wells by manually rotating two concentric layers without using external instruments. The Rotation-Chip is fabricated by a simple computer numerical control (CNC) machining process and is capable of carrying out 60 multiplexed reactions with a simple 30-degree or 60-degree rotation. Leveraging superhydrophobic coating, a high fluid transport efficiency of 92.78% is achieved without observable leaking. Integrated with an intracellular fluorescent assay, an on-chip detection limit of 1.8×106CFU/mL is achieved for ampicillin-resistantEscherichia coli (E. coli), which is similar to our off-chip results. We also develop a computer vision method to automatically distinguish positive and negative samples on the chip, showing 100% accuracy. Our Rotation-Chip is simple, low-cost, high-throughput, and can display test results with a single chip image, ideal for various multiplexing POC applications in resource-limited settings.

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“…This particular indirect cell patterning approach eliminates the need for a specific photo-mask and PDMS stamp in the conventional stamping technique. 70 Furthermore, the 3D bioprinting technique does not require a multi-step of chemical bonding such as plasma treatment or protein immobilization. 71 Hence, the material jetting bioprinting technique is a facile approach for fundamental cell-cell and cell-material interaction studies.…”

Section: Cell Printingmentioning

confidence: 99%

Improving printability of hydrogel-based bio-inks for thermal inkjet bioprinting applicationsviasaponification and heat treatment processes

Suntornnond

Huang

et al. 2022

J. Mater. Chem. B

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User‐friendly cell patterning methods using a polydimethylsiloxane mold with microchannels

Cited by 12 publications

References 56 publications

Superhydrophobic Rotation-Chip for Computer-Vision Identification of Drug-Resistant Bacteria

Superhydrophobic Rotation-Chip for Computer-Vision Identification of Drug-Resistant Bacteria

Superhydrophobic Rotation-Chip for Computer-Vision Identification of Drug-Resistant Bacteria

Improving printability of hydrogel-based bio-inks for thermal inkjet bioprinting applicationsviasaponification and heat treatment processes

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